AP1180 Anti-TLR5 Mouse mAb (4F7)

This Anti-TLR5 Mouse mAb (4F7) is validated for use in ELISA, Immunoblotting for the detection of TLR5.

Anti-TLR5 Mouse mAb (4F7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Toll-like Receptor 5

AP1180
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      AP1180-100UG
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          		 100 μg
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          Description
          OverviewRecognizes the TLR5 protein.
          Catalogue NumberAP1180
          Brand Family Calbiochem®
          SynonymsAnti-Toll-like Receptor 5
          Application Data
          Detection of human TLR5 by immunoblotting. Sample: Partial recombinant TLR5. Primary antibody: Anti-TLR5 Mouse mAb (4F7) (Cat. No. AP1180) (1-5 µg/ml). Detection: chemiluminescence.
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids of 517-635 human TLR5, expressed as a GST fusion protein
          Clone4F7
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-TLR5 Mouse mAb (4F7) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-October-2010 RFH
          SynonymsAnti-Toll-like Receptor 5
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Application Data
          Detection of human TLR5 by immunoblotting. Sample: Partial recombinant TLR5. Primary antibody: Anti-TLR5 Mouse mAb (4F7) (Cat. No. AP1180) (1-5 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes the TLR5 protein.
          BackgroundToll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.
          HostMouse
          ImmunogenA recombinant polypeptide corresponding to amino acids of 517-635 human TLR5, expressed as a GST fusion protein
          Clone4F7
          IsotypeIgG2b
          Specieshuman
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review