DR1041 Anti-TIP60 Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H Rb Polyclonal Antibody

Pricing & Availability

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DR1041-50UL
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      Description
      OverviewRecognizes the ~60 kDa TIP60 protein in Saos-2 cells.
      Catalogue NumberDR1041
      Brand Family Calbiochem®
      SynonymsAnti-HIV-1 Tat interactive protein, Anti-cPLA2-interaction protein
      Application Data
      Lane 1: Detection of human TIP60 by immunoblotting. Sample: Whole cell lysate (25 µg) from Saos-2 cells. Primary antibody: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:3000). Detection: chemiluminescence. Lanes 2 and 3: Detection of TIP60 by immunoprecipitation. Samples: Whole cell lysate (500 µg) from Saos-2 cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:100). Conditions for immunoblotting: same as lane 1.
      References
      ReferencesMurr, R., et al. 2006. Nat. Cell Biol. 8, 91.
      Sykes, S.M., et al. 2006. Mol. Cell 24, 841.
      Tang, Y., et al. 2006. Mol. Cell 24, 827.
      Frank, S.R., et al. 2003. EMBO Rep. 6, 575.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlSaos-2 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:3000)
      Immunoprecipitation (1:100)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human TIP60 protein
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-TIP60 Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TIP60 Rabbit pAb Certificates of Analysis

      TitleLot Number
      DR1041

      References

      Reference overview
      Murr, R., et al. 2006. Nat. Cell Biol. 8, 91.
      Sykes, S.M., et al. 2006. Mol. Cell 24, 841.
      Tang, Y., et al. 2006. Mol. Cell 24, 827.
      Frank, S.R., et al. 2003. EMBO Rep. 6, 575.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2007 RFH
      SynonymsAnti-HIV-1 Tat interactive protein, Anti-cPLA2-interaction protein
      ApplicationImmunoblotting (1:3000)
      Immunoprecipitation (1:100)
      Application Data
      Lane 1: Detection of human TIP60 by immunoblotting. Sample: Whole cell lysate (25 µg) from Saos-2 cells. Primary antibody: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:3000). Detection: chemiluminescence. Lanes 2 and 3: Detection of TIP60 by immunoprecipitation. Samples: Whole cell lysate (500 µg) from Saos-2 cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:100). Conditions for immunoblotting: same as lane 1.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~60 kDa TIP60 protein.
      BackgroundTIP60 (accession no. Q92993) is a 60 kDa protein that is a member of the MYST family, which is a distinct class of histone acetyl-transferases (HAT). TIP60 was initially identified as a protein that interacts with the HIV protein, Tat, and shares no homology with the HATs, CBP/p300, or PCAF. TIP60 was recently shown to modulate DNA repair and p53 function. TIP60 also directly acetylates γ-H2AX and, together with the cofactor, TRRAP, binds to the chromatin surrounding sites of DNA double-stranded breaks in vivo. Furthermore, TIP60 associates with MYC and recruits it to the chromatin, which results in MYC-induced actetylation of histone H4. TIP60 directly associates with p53 and specifically acetylates it at K120, modulating the decision between cell cycle arrest and apoptosis. TIP60 acts as a transcription coactivator of p53 that enhances the activation of p21 and puma, leading to cell growth arrest and apoptosis. K120 actetylation is essential for p53-mediated puma activation and apoptosis, but is dispensable for both p53-mediated growth arrest, through p21 activation, and p53/MDM2 interaction. K120 is not acetylated by p300 and its actetylation by TIP60 does not affect the stability or DNA binding activity of p53.
      HostRabbit
      Immunogenrecombinant, human TIP60 protein
      IsotypeIgG
      Specieshuman
      Positive controlSaos-2 cells
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMurr, R., et al. 2006. Nat. Cell Biol. 8, 91.
      Sykes, S.M., et al. 2006. Mol. Cell 24, 841.
      Tang, Y., et al. 2006. Mol. Cell 24, 827.
      Frank, S.R., et al. 2003. EMBO Rep. 6, 575.