IM32 Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
B, H, R M Monoclonal Antibody

Pricing & Availability

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IM32-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~28.5 kDa TIMP-1 protein. Also recognizes MMP/TIMP-1 complexes.
      Catalogue NumberIM32
      Brand Family Calbiochem®
      SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
      Application Data
      Detection of TIMP-1 by immunoblotting. Sample: Whole cell lysate from CHO cells. Primary antibody: Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) (Cat. No. IM32) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesFujimoto, N. and Iwata, K., 1994. Connective Tissue 26, 237.
      Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J. F., 1991. FASEB J. 5, 2145.
      Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.
      Product Information
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
      Positive controlrTIMP-1 Protein (Cat. Nos. PF019 or PF020)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Kodama, S., et al. 1990. J. Immunol. Meth. 127, 103. Imunohistochemistry Okada, Y., et al. 1990. Virchows Arch. B Cell Pathol. 59, 305. Paraffin Sections Romanic, A.M., et al. 1998. Stroke 29, 1020. Immunocytochemistry Zhao, W.Q., et al. 1998. J. Cell Sci. 111, 1147. Original Clone Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml, see application references)
      Immunocytochemistry (see application references)
      Paraffin Sections (25 µg/ml, see application references, see comments)
      Application CommentsAlso recognizes TIMP-1/MMP complexes.This antibody has been used to stain paraffin sections fixed with PLP (perioddate-lysine-4% paraformaldehyde) and 10% formaldehyde (using 25 µg/ml antibody). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified, bovine dental pulp TIMP-1
      ImmunogenBovine
      Clone7-6C1
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Bovine
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) Certificates of Analysis

      TitleLot Number
      IM32

      References

      Reference overview
      Fujimoto, N. and Iwata, K., 1994. Connective Tissue 26, 237.
      Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J. F., 1991. FASEB J. 5, 2145.
      Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
      ApplicationImmunoblotting (1 µg/ml, see application references)
      Immunocytochemistry (see application references)
      Paraffin Sections (25 µg/ml, see application references, see comments)
      Application Data
      Detection of TIMP-1 by immunoblotting. Sample: Whole cell lysate from CHO cells. Primary antibody: Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) (Cat. No. IM32) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody (see application references). Recognizes the ~28 kDa TIMP-1 protein.
      BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus and their activity is regulated by endogenous inhibitors. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 (also called EPA, Fibroblast collagenase inhibitor or collagenase inhibitor) is a 28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.
      HostMouse
      Immunogen speciesBovine
      Immunogenpurified, bovine dental pulp TIMP-1
      Clone7-6C1
      IsotypeIgG₁
      Speciesbovine, human, not mouse, rat
      Positive controlrTIMP-1 Protein (Cat. Nos. PF019 or PF020)
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAlso recognizes TIMP-1/MMP complexes.This antibody has been used to stain paraffin sections fixed with PLP (perioddate-lysine-4% paraformaldehyde) and 10% formaldehyde (using 25 µg/ml antibody). Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesFujimoto, N. and Iwata, K., 1994. Connective Tissue 26, 237.
      Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J. F., 1991. FASEB J. 5, 2145.
      Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.
      Application referencesImmunoblotting Kodama, S., et al. 1990. J. Immunol. Meth. 127, 103. Imunohistochemistry Okada, Y., et al. 1990. Virchows Arch. B Cell Pathol. 59, 305. Paraffin Sections Romanic, A.M., et al. 1998. Stroke 29, 1020. Immunocytochemistry Zhao, W.Q., et al. 1998. J. Cell Sci. 111, 1147. Original Clone Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.