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MAB5258-20UG Anti-Synaptophysin Antibody, clone SY38

MAB5258-20UG
20 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, B, F, H, M, R FC, ICC, IHC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB5258-20UG
      Replaces04-1019
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Synaptophysin Antibody, clone SY38
      Alternate Names
      • synaptophysin
      • Synaptophysin
      Background InformationSynaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non chromaffin paragangliomas.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control: Pancreas tissue
        Negative Control: Normal mouse serum
        Rat hippocampus tissue, mouse brain lysate.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 20 mM sodium phosphate buffer, 0.25 M NaCl, containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Synaptophysin using this Anti-Synaptophysin Antibody, clone SY38 validated for use in FC, IC, IH & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesFlow Cytometry (FACS):
      A previous lot of this antibody was used in Flow Cytometry.

      Western Blot:
      (Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein

      Immunohistochemistry:
      For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used.
      It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution.
      Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenVesicular fraction of bovine brain. The SY38 epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus & Betz 1990).
      CloneSY38
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12.
      IsotypeIgG1κ
      Species Reactivity
      • Avian
      • Bovine
      • Fish
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySynaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]
      Gene Symbol
      • SYP
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08247 # Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.
      SIZE: 313 amino acids; 33845 Da
      SUBUNIT: Homohexamer or homotetramer.
      SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
      DOMAIN: SwissProt: P08247 The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
      PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity).
      SIMILARITY: Belongs to the synaptophysin/synaptobrevin family. & Contains 1 MARVEL domain.
      Molecular Weight38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC from date of receipt. DO NOT FREEZE.
      Packaging Information
      Material Size20 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Synaptophysin Antibody, clone SY38 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Synaptophysin Antibody, clone SY38 Certificates of Analysis

      TitleLot Number
      Anti-Synaptophysin, clone SY38 - 21159892115989
      Anti-Synaptophysin, clone SY38 - 21217462121746
      Anti-Synaptophysin, clone SY38 - 23875322387532
      Anti-Synaptophysin, clone SY38 - 20350662035066
      Anti-Synaptophysin, clone SY38 - 21863342186334
      Anti-Synaptophysin, clone SY38 - 32139243213924
      Anti-Synaptophysin, clone SY38 - 34385623438562
      Anti-Synaptophysin, clone SY38 - 35414263541426
      Anti-Synaptophysin, clone SY38 - LV1424896LV1424896
      Anti-Synaptophysin, clone SY38 - LV1647005LV1647005

      References

      Reference overviewApplicationPub Med ID
      Synaptic dysregulation in a human iPS cell model of mental disorders.
      Wen, Z; Nguyen, HN; Guo, Z; Lalli, MA; Wang, X; Su, Y; Kim, NS; Yoon, KJ; Shin, J; Zhang, C; Makri, G; Nauen, D; Yu, H; Guzman, E; Chiang, CH; Yoritomo, N; Kaibuchi, K; Zou, J; Christian, KM; Cheng, L; Ross, CA; Margolis, RL; Chen, G; Kosik, KS; Song, H; Ming, GL
      Nature  2014

      Show Abstract
      25132547 25132547
      PDE5 inhibition improves object memory in standard housed rats but not in rats housed in an enriched environment: implications for memory models?
      Akkerman, S; Prickaerts, J; Bruder, AK; Wolfs, KH; De Vry, J; Vanmierlo, T; Blokland, A
      PloS one  9  e111692  2014

      Show Abstract
      25372140 25372140
      Quantitative proteomic and genetic analyses of the schizophrenia susceptibility factor dysbindin identify novel roles of the biogenesis of lysosome-related organelles complex 1.
      Gokhale, A; Larimore, J; Werner, E; So, L; Moreno-De-Luca, A; Lese-Martin, C; Lupashin, VV; Smith, Y; Faundez, V
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  3697-711  2012

      Show Abstract
      Immunofluorescence22423091 22423091
      Mutant SOD1 impairs axonal transport of choline acetyltransferase and acetylcholine release by sequestering KAP3.
      Tateno, M; Kato, S; Sakurai, T; Nukina, N; Takahashi, R; Araki, T
      Human molecular genetics  18  942-55  2009

      Show Abstract
      Western Blotting19088126 19088126
      ABCG1 and ABCG4 are coexpressed in neurons and astrocytes of the CNS and regulate cholesterol homeostasis through SREBP-2.
      Tarr, Paul T and Edwards, Peter A
      J. Lipid Res., 49: 169-82 (2008)  2008

      Show Abstract
      17916878 17916878
      Phosphatidylinositol-4-kinase type II alpha is a component of adaptor protein-3-derived vesicles
      Salazar, Gloria, et al
      Mol Biol Cell, 16:3692-704 (2005)  2005

      15944223 15944223
      Differentiation of human embryonic stem cells to dopaminergic neurons in serum-free suspension culture.
      Thomas C Schulz, Scott A Noggle, Gail M Palmarini, Deb A Weiler, Ian G Lyons, Kate A Pensa, Adrian C B Meedeniya, Bruce P Davidson, Nevin A Lambert, Brian G Condie
      Stem cells (Dayton, Ohio)  22  1218-38  2004

      Show Abstract
      15579641 15579641
      beta-amyloid peptides enhance alpha-synuclein accumulation and neuronal deficits in a transgenic mouse model linking Alzheimer's disease and Parkinson's disease.
      Masliah, E; Rockenstein, E; Veinbergs, I; Sagara, Y; Mallory, M; Hashimoto, M; Mucke, L
      Proceedings of the National Academy of Sciences of the United States of America  98  12245-50  2001

      Show Abstract
      11572944 11572944

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies