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MAB329-C Anti-Synaptophysin Antibody, clone SP15 (Ascites Free)

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MAB329-C
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Mk, M, Fe WB, IHC, ICC, ELISA M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB329-C
      DescriptionAnti-Synaptophysin Antibody, clone SP15 (Ascites Free)
      Alternate Names
      • Major synaptic vesicle protein p38
      • Synaptophysin
      Background InformationSynaptophysin (UniProt P08247; also known as Major synaptic vesicle protein p38) is encoded by the SYP (also known as MRX96, MRXSYP) gene (Gene ID 6855) in human. Synaptophysin is a 38 kDa four-transmembrane glycoprotein on synaptic vesicle (SV) with both its N- and C-termini exposed cytoplasmically. Synaptophysin forms homomultimers in the SV membrane and is heavily phosphorylated on tyrosine residues in its C-terminal pentapeptide repeats. Synaptophysin is also known to interact with SV v-SNARE protein synaptobrevin II (sybII) when sybII is not engaged with other SNARE proteins, suggesting a role of synaptophysin in sybII retrieval during SV endocytosis. Consistently, synaptophysin-knockout neurons display reduced levels of sybII at their nerve terminals.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Synaptophysin Antibody, clone SP15 (Ascites Free) is validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, ELISA for the detection of Synaptophysin.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      • ELISA
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected Synaptophysin in 10 µg of human brain tissue lysate.
      Western Blotting Analysis: A representative lot detected synaptophysin in rat hippocampal tissue extracts (Lin, D., et al. (2012). Behav. Brain Res. 228(2):319-327).
      Western Blotting Analysis: Representative lots detected synaptophysin in mouse brain synaptosomes preparations (Shim, S.Y., et al. (2008). J. Neurosci. 28(14):3604-3614).
      Western Blotting Analysis: A representative lot detected synaptophysin in the large dense-core vesicles-containing fractions obtained by sucrose gradient centrifugation of rat brain median eminence (ME) neuroterminals preparations (Yin, W., et al. (2007). Exp. Biol. Med. (Maywood). 232(5):662-673).
      Immunohistochemistry Analysis: A representative lot detected a stronger synaptophysin immunoreactivity in the inner molecular layer of frozen dentate gyrus sections from a 3-week old monkey, while a stronger immunoreactivity was seen in the outer molecular layer of dentate gyrus sections from a 13-year old monkey (Lavenex, P., et al. (2007). Dev. Neurosci. 29(1-2):179–192).
      Immunohistochemistry Analysis: A representative lot immunostained nerve terminals at palisade ending using cat extraocular muscle (EOM) whole mount sections (Konakci, K.Z., et al. (2005). Invest. Ophthalmol. Vis. Sci. 46(1):155-165).
      Immunohistochemistry Analysis: A representative lot detected synaptophysin immunoreactivity in human brain tissue sections (Honer, W.G., et al. (1993). Brain Res. 609(1-2):9-20).
      Immunocytochemistry Analysis: A representative lot detected punctate synatophysin immunoreactivity among cultured primary cerebrocortical neurons from rat embryos (Bragina, L., et al. (2006). J. Neurochem. 99(1):134-141.).
      ELISA Analysis: Representative lots were employed as the capture antibody for the detection of synaptophysin in human temporal cortex, frontal cortex, and cerebellar cortex extracts by sandwich ELISA (Klucken, J., et al. (2006). Acta Neuropathol. 111(2):101-108; Fukumoto, H., et al. (2002). Arch. Neurol. 59(9):1381-1389).
      Biological Information
      ImmunogenHuman brain proteins immunoprecipitated with EP10 (Cat. No. MAB332).
      CloneSP15
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgMκ
      Species Reactivity
      • Human
      • Rat
      • Monkey
      • Mouse
      • Feline
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SYP
      Purification MethodPurified by Ion-Exchange Chromatography
      UniProt Number
      Molecular Weight~34 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptophysin in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Synaptophysin Antibody, clone SP15 (Ascites Free) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Synaptophysin Antibody, clone SP15 (Ascites Free) Certificates of Analysis

      TitleLot Number
      Anti-Synaptophysin, clone SP15 (Ascites Free) - 32672853267285
      Anti-Synaptophysin, clone SP15 (Ascites Free) - 35115883511588
      Anti-Synaptophysin, clone SP15 (Ascites Free) -28570012857001
      Anti-Synaptophysin, clone SP15 (Ascites Free) -Q2621715Q2621715
      Anti-Synaptophysin, clone SP15 (Ascites Free) Monoclonal Antibody2986014
      Anti-Synaptophysin, clone SP15 -27695442769544

      References

      Reference overviewSpeciesPub Med ID
      Lidocaine attenuates cognitive impairment after isoflurane anesthesia in old rats.
      Lin, D; Cao, L; Wang, Z; Li, J; Washington, JM; Zuo, Z
      Behavioural brain research  228  319-27  2012

      Show Abstract
      22192381 22192381
      Protein 600 is a microtubule/endoplasmic reticulum-associated protein in CNS neurons.
      Shim, SY; Wang, J; Asada, N; Neumayer, G; Tran, HC; Ishiguro, K; Sanada, K; Nakatani, Y; Nguyen, MD
      The Journal of neuroscience : the official journal of the Society for Neuroscience  28  3604-14  2008

      Show Abstract
      18385319 18385319
      Novel localization of NMDA receptors within neuroendocrine gonadotropin-releasing hormone terminals.
      Yin, W; Mendenhall, JM; Bratton, SB; Oung, T; Janssen, WG; Morrison, JH; Gore, AC
      Experimental biology and medicine (Maywood, N.J.)  232  662-73  2007

      Show Abstract
      17463163 17463163
      Postnatal development of the primate hippocampal formation.
      Lavenex, P; Banta Lavenex, P; Amaral, DG
      Developmental neuroscience  29  179-92  2007

      Show Abstract
      17148960 17148960
      Clinical and biochemical correlates of insoluble alpha-synuclein in dementia with Lewy bodies.
      Klucken, J; Ingelsson, M; Shin, Y; Irizarry, MC; Hedley-Whyte, ET; Frosch, M; Growdon, J; McLean, P; Hyman, BT
      Acta neuropathologica  111  101-8  2006

      Show Abstract
      16482476 16482476
      GLT-1 down-regulation induced by clozapine in rat frontal cortex is associated with synaptophysin up-regulation.
      Bragina, L; Melone, M; Fattorini, G; Torres-Ramos, M; Vallejo-Illarramendi, A; Matute, C; Conti, F
      Journal of neurochemistry  99  134-41  2006

      Show Abstract
      Rat16987241 16987241
      Molecular characteristics suggest an effector function of palisade endings in extraocular muscles.
      Konakci, KZ; Streicher, J; Hoetzenecker, W; Blumer, MJ; Lukas, JR; Blumer, R
      Investigative ophthalmology & visual science  46  155-65  2005

      Show Abstract
      15623769 15623769
      Beta-secretase protein and activity are increased in the neocortex in Alzheimer disease.
      Fukumoto, H; Cheung, BS; Hyman, BT; Irizarry, MC
      Archives of neurology  59  1381-9  2002

      Show Abstract
      12223024 12223024
      Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.
      Honer, WG; Hu, L; Davies, P
      Brain research  609  9-20  1993

      Show Abstract
      7685234 7685234

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      Title
      Neuroscience Solutions for productive research
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      Title
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies