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AB5480 Anti-Superoxide Dismutase 1 Antibody, aa 25-37

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AB5480
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB5480
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Superoxide Dismutase 1 Antibody, aa 25-37
      Alternate Names
      • SOD1
      Background InformationMutations in the copper/ zinc superoxide dismutase (SOD1) gene are associated with 15- 20% of the familial forms of motoneuron disease. This 153 amino acid metalloenzyme is expressed in virtually all cells of all organisms above bacteria and is highly conserved across species, although some minor variations do occur. The incorporation of the mutated form of the SOD1 human gene into a transgenic mouse leads to the onset of the disease that closely resembles the human condition. These animals become weak at about 2-4 months of age and rapidly lose function, which results in death 4- 6 weeks later.
      References
      Product Information
      FormatSerum
      PresentationRabbit serum. Lyophilized. Contains no preservative. Reconstitute with 50 μL of sterile water.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Superoxide Dismutase 1 Antibody, aa 25-37 detects level of Superoxide Dismutase 1 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blotting: 1:500

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 25-37 of human SOD1.
      Epitopeaa 25-37
      HostRabbit
      SpecificitySuperoxide Dismutase 1 (SOD1), human specific.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
      Gene Symbol
      • SOD1
      • ALS1
      • IPOA
      • Cu-Zn
      • SOD
      • ALS
      • homodimer
      • EC 1.15.1.1
      Non-Reactive Species
      • Mouse
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00441 # Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
      COFACTOR: Binds 1 copper ion per subunit. & Binds 1 zinc ion per subunit.
      SIZE: 154 amino acids; 15936 Da
      SUBUNIT: Homodimer. The pathogenics variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not.
      SUBCELLULAR LOCATION: Cytoplasm.
      PTM: Unlike wild-type protein, the pathogenics variants ALS1 Arg- 38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A; which leads to their proteasomal degradation.
      DISEASE: SwissProt: P00441 # Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
      SIMILARITY: SwissProt: P00441 ## Belongs to the Cu-Zn superoxide dismutase family.
      MISCELLANEOUS: Zinc binding promotes dimerization.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -70°C (dry) for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Superoxide Dismutase 1 Antibody, aa 25-37 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Superoxide Dismutase 1 Antibody, aa 25-37 Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-SUPEROXIDE DISMUTASE 1 (SOD1) HUMAN SPECIFIC POLYCLONAL ANTIBODY - 2424086 2424086
      RABBIT ANTI-SUPEROXIDE DISMUTASE 1 (SOD1) HUMAN SPECIFIC POLYCLONAL ANTIBODY - 2435073 2435073
      RABBIT ANTI-SUPEROXIDE DISMUTASE 1 (SOD1) - 3180468 3180468
      RABBIT ANTI-SUPEROXIDE DISMUTASE 1 (SOD1) -2594328 2594328
      RABBIT ANTI-SUPEROXIDE DISMUTASE 1 (SOD1) -2703732 2703732
      RABBIT ANTI-SUPEROXIDE DISMUTASE 1 (SOD1) HUMAN SPECIFIC POLYCLONAL ANTIBODY - 2188445 2188445
      RABBIT ANTI-SUPEROXIDE DISMUTASE 1 (SOD1) HUMAN SPECIFIC POLYCLONAL ANTIBODY - 2251745 2251745

      References

      Reference overviewPub Med ID
      Nerve terminal degeneration is independent of muscle fiber genotype in SOD1 mice.
      Carrasco, DI; Bichler, EK; Seburn, KL; Pinter, MJ
      PloS one  5  e9802  2010

      Show Abstract
      20339550 20339550
      Adherent monomer-misfolded SOD1.
      Watanabe, Y; Morita, E; Fukada, Y; Doi, K; Yasui, K; Kitayama, M; Nakano, T; Nakashima, K
      PloS one  3  e3497  2008

      Show Abstract Full Text Article
      18946506 18946506
      Development and characterization of human and mouse specific antibodies to CuZn-superoxide dismutase (SOD1).
      Bartlett, S E, et al.
      J. Neurosci. Methods, 98: 63-7 (2000)  2000

      Show Abstract
      10837872 10837872

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies