AB15810 Anti-Substance P Receptor Antibody

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AB15810
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R ICC, IF, IHC, WB Gp Unpurified Polyclonal Antibody
      Description
      Catalogue NumberAB15810
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Substance P Receptor Antibody
      Alternate Names
      • Substance-P receptor
      • Neurokinin-1 receptor
      • NK-1 receptor
      • NK-1R
      • SPR
      • Tachykinin receptor 1
      Background InformationSubstance-P receptor (UniProt P14600; also known as Neurokinin-1 receptor, NK-1 receptor, NK-1R, SPR, Tachykinin receptor 1) is encoded by the Tacr1 (also known as Nk1r, Tac1r) gene (Gene ID 24807) in rat species. NK-1, NK-2 and NK-3 are receptors that mediate the biological activities of tachykinins, including substance P (SP), neurokinin A (NKA), neurokinin B (NKB), ranakinin, kassinin, neuropeptide gamma, and eledoisin, widely distributed in the central and peripheral nervous systems. SP shows the highest affinity toward NK-1R (substance-P receptor) and exerts its biological activities via NK-1R, including addition, depression, chronic inflammation, and cancer progression. The involvement of NK-1R in acute pancreatitis through mediating neurogenic inflammation has also been reported. Upon stimulation by SP, the NK-1R interacts with multiple G proteins, including Gαs, Gαq/11, Gαi/o, Gα12, and Gα13, generating second messengers and activating downstream effector molecules. After binding NK-1R, SP induces a clathrin-dependent internalization of the receptor-ligand complex, SP is then degraded and the receptor is recycled to the cell surface. NK-1R is a 7-transmembrane (32-54, 65-86, 107-128, 149-169, 195-219, 249-270, 284-308) GPCR, having its N-terminal end (a.a. 1-31) at the extracelluar side and C-terminal end (a.a. 309-407) at the cytoplasmic side. There exist a second alternatively spliced human NK-1R isoform that lacks the C-terminal cytoplasmic domain (a.a. 312-407 missing). The full-length form enhances the growth of cancer cells and stimulates the production of cytokines, which up-regulate the truncated form. The truncated form mediates malignancy and is more abundant in colonic epithelial cells from patients with colitis-associated cancer. In cancer cells, the NK-1 receptor is mainly localized on the plasma membrane and in cytoplasm, and occasionally also in the nucleus.
      References
      Product Information
      FormatUnpurified
      Control
      • Rat brain tissue lysate
      PresentationGuinea Pig polyclonal antibody serum with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationThis Guinea pig polyclonal Anti-Substance P Receptor Antibody, Cat. No. AB15810 detects levels of Substance P receptor, and has been published and validated for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected substance P receptor in 10 µg of human brain tissue lysate.

      Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected substance P receptor in rat brain tissue sections.

      Immunofluorescence Analysis: A 1:5,000-10,000 dilution from a representative lot detected substance-P receptor in frozen rat spinal cord tissue sections (25 µm cryostat) fixed with 4% paraformaldehyde containing 0.18% picric acid by indirect immunofluorescence staining using a rhodamine Red-X-conjugated secondary antibody.

      Immunofluorescence Analysis: A representative lot detected substance-P receptor- (NK-1R) positive neurons by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed and 0.2% Triton X-100-permeabilized mouse brain cryosections (Manzke, T., et la. (2010). J. Clin. Invest. 120(11):4118-4128).

      Immunocytochemistry Analysis: A representative lot detected substance-P receptor (NK-1R) by fluorescent immunocytochemistry staining of cultured primary E17-E18 rat embryonic amygdala neurons fixed with 4% paraformaldehyde and permeabilized with 0.1% saponin (Yu, Y.J., et al. (2009). J. Neurosci. 29(1):222-233).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminal end sequence of rat substance P receptor.
      EpitopeC-Terminus
      HostGuinea Pig
      SpecificityThis guinea pig polyclonal antiserum targets substance P receptor C-terminal end sequence. Target region is absent in human spliced isoform 2.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tacr1
      • Tac1r
      • Nk1r
      Purification MethodUnpurified.
      UniProt Number
      Molecular Weight~58/54 kDa observed. Target band size appears larger than the calculated molecular weights of 46.25/46.32/46.37 kDa (human/mouse/rat) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: A 1:5000 dilution from a representative lot detected substance-P receptor in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Substance P Receptor Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Substance P Receptor Antibody Certificates of Analysis

      TitleLot Number
      ANTI-SUBSTANCE P RECEPTOR - LV1513951LV1513951
      Anti-Substance P Receptor -
      Anti-Substance P Receptor - 21470622147062
      Anti-Substance P Receptor - 23923062392306
      Anti-Substance P Receptor - 20318112031811
      Anti-Substance P Receptor - 21020002102000
      Anti-Substance P Receptor - 22119402211940
      Anti-Substance P Receptor - 32887253288725
      Anti-Substance P Receptor - 35083823508382
      Anti-Substance P Receptor - LV1587443LV1587443

      References

      Reference overviewPub Med ID
      VEGF induces sensory and motor peripheral plasticity, alters bladder function, and promotes visceral sensitivity.
      Malykhina, AP; Lei, Q; Erickson, CS; Epstein, ML; Saban, MR; Davis, CA; Saban, R
      BMC physiology  12  15  2012

      Show Abstract
      23249422 23249422
      VEGF signaling mediates bladder neuroplasticity and inflammation in response to BCG.
      Saban, MR; Davis, CA; Avelino, A; Cruz, F; Maier, J; Bjorling, DE; Sferra, TJ; Hurst, RE; Saban, R
      BMC physiology  11  16  2011

      Show Abstract
      22059553 22059553
      Conserved long noncoding RNAs transcriptionally regulated by Oct4 and Nanog modulate pluripotency in mouse embryonic stem cells.
      Sheik Mohamed J, Gaughwin PM, Lim B, Robson P, Lipovich L
      RNA  16  324-37. Epub 2009 Dec 21.  2010

      Show Abstract Full Text Article
      20026622 20026622
      Serotonin receptor 1A-modulated phosphorylation of glycine receptor α3 controls breathing in mice.
      Manzke, T; Niebert, M; Koch, UR; Caley, A; Vogelgesang, S; Hülsmann, S; Ponimaskin, E; Müller, U; Smart, TG; Harvey, RJ; Richter, DW
      The Journal of clinical investigation  120  4118-28  2010

      Show Abstract
      20978350 20978350
      Neurokinin 1 receptors regulate morphine-induced endocytosis and desensitization of mu-opioid receptors in CNS neurons.
      Yu, YJ; Arttamangkul, S; Evans, CJ; Williams, JT; von Zastrow, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  29  222-33  2009

      Show Abstract
      19129399 19129399