AB962 Anti-Substance P Antibody, pain

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AB962
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, Op IH(P) Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB962
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Substance P Antibody, pain
      References
      Product Information
      FormatSerum
      PresentationLiquid antiserum. Contains 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Substance P Antibody, pain is an antibody against Substance P for use in IH(P).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesTISSUE EVALUATION:

      Formalin-fixed and paraffin-embedded sections (6 microns thick) of median eminence tissue were prepared from the domestic chicken and opossum. Cryostat sections of the above tissues can also be used in the staining procedure.



      IMMUNOPEROXIDASE

      STAINING:

      1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4).

      2. The antiserum diluted 1:500 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.

      3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these two steps excess reagent is washed from the section as described before.

      4. The final step in the staining involved treating section with a mixture of 3,3'-diamino-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.



      IMMUNOFLUORESCENCE

      STAINING:

      1. Same as Step 1 above.

      2. The tissue is reacted with the antiserum, diluted 1:20 in (PBS) for 30 minutes at 37*C.

      3. After washing off the excess antiserum with PBS, the section is treated with fluorescein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.



      COMMENTS:

      The antiserum described here has been established to give a positive staining of the nerve fibers in the medium eminence of two species using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time, e.g. 24 hours.
      Biological Information
      ImmunogenSynthetic substance P bound to keyhole limpet hemocyanin (KLH) with carbodiimide.
      HostRabbit
      SpecificityThe antiserum has been evaluated for its ability to stain nerve fibers in the external median eminence of the chicken and opossum using both the immunoperoxidase and immunofluorescence procedures.
      Species Reactivity
      • Chicken
      • Opossum
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • TAC1
      • NKNA
      • Hs.2563
      • NK2
      • PPT
      • NKA
      • TAC2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20366 # Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
      SIZE: 129 amino acids; 15003 Da
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P20366 ## Belongs to the tachykinin family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Substance P Antibody, pain SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Substance P Antibody, pain Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-SUBSTANCE P POLYCLONAL ANTIBODY - 23970352397035
      RABBIT ANTI-SUBSTANCE P - 24946162494616
      RABBIT ANTI-SUBSTANCE P -27597222759722
      RABBIT ANTI-SUBSTANCE P -28177392817739

      References

      Reference overviewPub Med ID
      Enhanced nerve-stimulated muscarinic and neurokinin contractions of ileum from streptozotocin guinea-pigs.
      J Cellini,R Pommier,R Porter,K J Lepard
      Autonomic & autacoid pharmacology  32  2012

      Show Abstract
      22676206 22676206
      Structural basis for control of secondary vessels in the long-finned eel Anguilla reinhardtii.
      Skov, PV; Bennett, MB
      The Journal of experimental biology  207  3339-48  2004

      Show Abstract
      15326210 15326210
      A novel technique for quantitative immunohistochemical imaging of various neurochemicals in a multiple-stained brain slice.
      Sutoo D, Akiyama K, Yabe K.
      Journal of neuroscience methods  118  41-50  2002

      Show Abstract
      12191756 12191756
      Substance P immunoreactivity in the median eminence of the North American opossum and domestic fowl.
      Ho, R H and DePalatis, L R
      Brain Res., 189: 565-9 (1980)  1980

      6154507 6154507
      Experimental immunohistochemical studies on the localization and distribution of substance P in cat primary sensory neurons.
      Hökfelt, T, et al.
      Brain Res., 100: 235-52 (1975)  1975

      Show Abstract
      1104079 1104079

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies