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AB5535-AF488 Anti-Sox9 Antibody, Alexa Fluor® 488 conjugate

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, M, ChICCRbAlexaFluor®488Polyclonal Antibody
      Catalogue NumberAB5535-AF488
      DescriptionAnti-Sox9 Antibody, Alexa Fluor® 488 conjugate
      Alternate Names
      • Transcription factor SOX-9
      • Sox9
      Background InformationTranscription factor SOX-9 (UniProt P48436; also known as Sex-determining region Y-box 9 protein, SRY-related HMG-box gene 9) is encoded by the SOX9 (also known as CMD1, CMPD1, SRA1) gene (Gene ID 6662) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences that encode for the HMG-box domains that mediate sequence-specific DNA binding. Sox genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at at least 40 genomic loci and at least 30 Sox genes have been identified.
      Product Information
      PresentationPurified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
      Quality LevelMQ100
      ApplicationThis Anti-Sox9 Antibody, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of Sox9.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sox9 in HepG2 cells.
      The unconjugated version (Cat. No. AB5535) is also shown to be suitable for Western blotting and immunohistochemistry applications.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Sox9.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Rat
      • Human
      • Mouse
      • Chicken
      Species Reactivity NoteRat, Human, Mouse, Chicken. Predicted to react with Bovine, Feline, Horse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SOX9
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight55-65 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sox9 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-Sox9 Antibody, Alexa Fluor® 488 conjugate SDS


      Safety Data Sheet (SDS) 

      Anti-Sox9 Antibody, Alexa Fluor® 488 conjugate Certificates of Analysis

      TitleLot Number
      Anti-Sox9, Alexa Fluor® 488 conjugate 2895556
      Anti-Sox9, Alexa Fluor® 488 conjugate - 3128128 3128128
      Anti-Sox9, Alexa Fluor® 488 conjugate - 3229373 3229373
      Anti-Sox9, Alexa Fluor® 488 conjugate - 3384386 3384386
      Anti-Sox9, Alexa Fluor® 488 conjugate - 3727570 3727570
      Anti-Sox9, Alexa Fluor® 488 conjugate - 3764361 3764361
      Anti-Sox9, Alexa Fluor® 488 conjugate -2860935 2860935
      Anti-Sox9, Alexa Fluor® 488 conjugate -Q2595867 Q2595867
      Anti-Sox9, Alexa Fluor® 488 conjugate_2836788 2836788

      Technical Info

      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage. (EMD)

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      Life Science Research > Antibodies and Assays > Primary Antibodies