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AB5535 Anti-Sox9 Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, ChIHC, WB, ChIP, ChIP-seq, ICC, IFRbAffinity PurifiedPolyclonal Antibody
      Catalogue NumberAB5535
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Sox9 Antibody
      Background InformationSox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
      Product Information
      FormatAffinity Purified
      • Embryonic tissue, Adult chondrocytes.
      PresentationPurified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide
      Quality LevelMQ100
      ApplicationAnti-Sox9 Antibody is a well characterized affinity purified Rabbit Polyclonal Antibody that reliably detects Transcription Factor Sox-9. This highly published antibody has been validated in IHC & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected Sox9 in murine embryonic bone and adult cartilage tissue sections.
      Western Blotting Analysis: An 1:500 dilution from a representative lot detected Sox9 in human PC3 prostate cancer cells and HepG2 hepatocytes.
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at target chromatin sites by ChIP using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243).
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at the Bmi promoter in Z/sox9tg but not in wild-type control mouse embryonic fibroblasts/MEFs (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
      ChIP-sequencing (ChIP-seq) Analysis: A representative lot detected Sox9-targeted chromatin sites by a genome-wide ChIP-seq analysis using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243).
      Immunofluorescence Analysis: A representative lot detected the accumulation of Sox9-positive oval cells by fluorescent immunohistochemistry staining of paraffin-embedded liver sections from transgenic mice treated with diethylnitrosamine to induce conditional liver HNF4a knockout (Saha, S.K., et al. (2014). Nature. 513(7516):110-114).
      Immunofluorescence Analysis: Representative lots detected Sox9 immunoreactivity in paraffin-embedded mouse embryo sections by fluorescent immunohistochemistry (Carrasco, M., et al. (2012). J. Clin. Invest. 122(10):3504-3515; Sylva, M., et al. (2011). PLoS One. 6(8):e22616).
      Immunofluorescence Analysis: Representative lots immunostained Müller glial cells in frozen mouse and chicken retina sections by fluorescent immunohistochemistry staining of (Muranishi, Y., and Furukawa, T. (2012). J. Biomed. Biotechnol. 2012:973140; Fischer, A.J., et al. (2011). Neuroscience. 178:250-260).
      Immunocytochemistry Analysis: A representative lot detected the stem cell marker Sox9 by fluorescent immunocytochemistry staining of paraformaldehyde-fixed E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441).
      Immunohistochemistry Analysis: A representative lot immunostained the supporting cells (Sertoli) of the seminiferous tubules by immunohistochemistry staining of paraffin-embedded mouse testis sections (O'Shaughnessy, P.J., et al. (2012). PLoS One. 7(4):e35136).
      Immunohistochemistry Analysis: A representative lot detected Sox9 immunoreactivity in various formalin-fixed, paraffin-embedded human tumor tissue sections (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
      Western Blotting Analysis: A representative lot detected the stem cell marker Sox9 in E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441).
      Western Blotting Analysis: A representative lot detected upregulated Sox9 expression level in human colorectal cancer cell lines, HCT116, DLD1, and SW620 (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminal sequence of human Sox9.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes Sox9.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Chicken
      Species Reactivity NoteHuman, Mouse, Rat, Chicken. Predicted to react with Bovine, Equine, Feline, and Ovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
      Gene Symbol
      • SOX9
      • CMPD1
      • CMD1
      • SRA1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P48436 # Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
      SIZE: 509 amino acids; 56137 Da
      SUBCELLULAR LOCATION: Nucleus (Potential).
      DISEASE: SwissProt: P48436 # Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male- to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
      SIMILARITY: SwissProt: P48436 ## Contains 1 HMG box DNA-binding domain
      Molecular Weight56-65 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: An 1:2000 dilution of this antibody detected Sox9 in HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-Sox9 Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-Sox9 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Sox9 3063352
      Anti-Sox9 - 2383973 2383973
      Anti-Sox9 - 2424751 2424751
      Anti-Sox9 - 2462860 2462860
      Anti-Sox9 - 1969702 1969702
      Anti-Sox9 - 1997395 1997395
      Anti-Sox9 - 2031330 2031330
      Anti-Sox9 - 2065999 2065999
      Anti-Sox9 - 2167153 2167153
      Anti-Sox9 - 2262679 2262679


      Reference overviewApplicationSpeciesPub Med ID
      Characterization of the neural stem cell gene regulatory network identifies OLIG2 as a multifunctional regulator of self-renewal.
      Mateo, JL; van den Berg, DL; Haeussler, M; Drechsel, D; Gaber, ZB; Castro, DS; Robson, P; Crawford, GE; Flicek, P; Ettwiller, L; Wittbrodt, J; Guillemot, F; Martynoga, B
      Genome research  25  41-56  2015

      Show Abstract
      25294244 25294244
      Hair follicle bulge cultures yield class III β-tubulin-positive melanoglial cells.
      Locher, H; Saadah, N; de Groot, S; de Groot, JC; Frijns, JH; Huisman, MA
      Histochemistry and cell biology  144  87-91  2015

      Show Abstract
      Immunocytochemistry25724811 25724811
      The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma.
      Krah, NM; De La O, JP; Swift, GH; Hoang, CQ; Willet, SG; Chen Pan, F; Cash, GM; Bronner, MP; Wright, CV; MacDonald, RJ; Murtaugh, LC
      eLife  4  2015

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      26151762 26151762
      LINE-1 Mediated Insertion into Poc1a (Protein of Centriole 1 A) Causes Growth Insufficiency and Male Infertility in Mice.
      Geister, KA; Brinkmeier, ML; Cheung, LY; Wendt, J; Oatley, MJ; Burgess, DL; Kozloff, KM; Cavalcoli, JD; Oatley, JM; Camper, SA
      PLoS genetics  11  e1005569  2015

      Show Abstract
      26496357 26496357
      Spontaneous development of hepatocellular carcinoma with cancer stem cell properties in PR-SET7-deficient livers.
      Nikolaou, KC; Moulos, P; Chalepakis, G; Hatzis, P; Oda, H; Reinberg, D; Talianidis, I
      The EMBO journal  34  430-47  2015

      Show Abstract
      25515659 25515659
      Lymphangioleiomyomatosis Biomarkers Linked to Lung Metastatic Potential and Cell Stemness.
      Ruiz de Garibay, G; Herranz, C; Llorente, A; Boni, J; Serra-Musach, J; Mateo, F; Aguilar, H; Gómez-Baldó, L; Petit, A; Vidal, A; Climent, F; Hernández-Losa, J; Cordero, Á; González-Suárez, E; Sánchez-Mut, JV; Esteller, M; Llatjós, R; Varela, M; López, JI; García, N; Extremera, AI; Gumà, A; Ortega, R; Plà, MJ; Fernández, A; Pernas, S; Falo, C; Morilla, I; Campos, M; Gil, M; Román, A; Molina-Molina, M; Ussetti, P; Laporta, R; Valenzuela, C; Ancochea, J; Xaubet, A; Casanova, Á; Pujana, MA
      PloS one  10  e0132546  2015

      Show Abstract
      26167915 26167915
      Neurite Mistargeting and Inverse Order of Intraretinal Vascular Plexus Formation Precede Subretinal Vascularization in Vldlr Mutant Mice.
      Johnson, V; Xiang, M; Chen, Z; Junge, HJ
      PloS one  10  e0132013  2015

      Show Abstract
      26177550 26177550
      Key pathways regulated by HoxA9,10,11/HoxD9,10,11 during limb development.
      Raines, AM; Magella, B; Adam, M; Potter, SS
      BMC developmental biology  15  28  2015

      Show Abstract
      26186931 26186931
      Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach.
      Debowski, K; Warthemann, R; Lentes, J; Salinas-Riester, G; Dressel, R; Langenstroth, D; Gromoll, J; Sasaki, E; Behr, R
      PloS one  10  e0118424  2015

      Show Abstract
      Immunofluorescence25785453 25785453
      Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung.
      Galvis, LA; Holik, AZ; Short, KM; Pasquet, J; Lun, AT; Blewitt, ME; Smyth, IM; Ritchie, ME; Asselin-Labat, ML
      Development (Cambridge, England)  142  1458-69  2015

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      25790853 25790853


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