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AB5603 Anti-Sox2 Antibody

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AB5603
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IF, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5603
      ReplacesAB5770
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Sox2 Antibody
      Alternate Names
      • Transcription factor SOX-2
      • Sex determining region Y-box 2
      • SRY-related HMG-box gene 2
      • Transcription factor SOX2
      Background InformationTranscription factor SOX-2 (UniProt: P48431; also known as SOX-2, Sex determining region Y-box 2, SRY-related HMG-box gene 2) is encoded by the SOX2 gene (Gene ID: 6657) in human. SOX-2 is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is shown to be critical for early embryogenesis and for embryonic stem cell pluripotency. It may function as a switch in neuronal development. It allows neural cells in undifferentiated state by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Sumoylation of SOX-2 is shown to inhibit binding on DNA and negatively regulate the FGF4 transactivation. Defects in SOX2 gene are linked to microphthalmia that is characterized by eye malformation ranging from small eye size of a single eye to complete bilateral absence of ocular tissue.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Immunoblot: Mouse or human embryonic stem cell lysate, mouse embryonic germ cell lysate
        Immunocytochemistry: Human embryonic stem cells (H9 line)
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SOX2 Antibody, Cat. No. AB5603, is a highly specific rabbit polyclonal antibody SOX2 and has been tested for use in Immunocytochemistry, and Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: 10 µg/mL from a representative lot detected Sox2 in H9 human stem cells.
      Immunocytochemistry Analysis: A representative lot detected nuclear Sox2 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human embryonic stem cells (hESCs) by fluorescent immunocytochemistry (Kingham, E., et al. (2013). Small. 9(12):2140-2151).
      Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed iPSCs generated from human kidney tubular renal epithelial cells (Montserrat, N., et al. (2012). J. Biol. Chem. 287(29):24131-24138).
      Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized iPSCs generated from the skin cells of patients with Parkinson's disease (Sánchez-Danés, A., et al. (2012). EMBO Mol. Med. 4(5):380-395).
      Immunocytochemistry Analysis: Representative lots detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized iPSCs generated from mouse ear cells and MEF (Burns, J.C., et al. (2012). PLoS One. 7(10):e48704; Hadadeh, O., et al. (2012). PLoS One. 7(11):e49065).
      Western Blotting Analysis: A representative lot detected Sox2 in mouse embryonic stem cell (mESC) nuclear extracts (Banaszynski, L.A., et al. (2013). Cell. 155(1):107-120).
      Immunofluorescence Analysis: A representative lot detected Sox2-positive cells in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized mouse brain vibratome sections by fluorescent immunohistochemistry (Matsuda, S., et al. (2012). J. Neurosci. 32(36):12543-12557).
      Immunohistochemistry Analysis: A representative lot detected Sox2 immunoreactivity in 4% paraformaldehyde-fixed whole mount mouse embryo sections (van Rooijen, C., et al. Development. 139(14):2576-2583).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal region sequence of human Sox2.
      EpitopeC-terminal region.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Sox2 C-terminal region.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Bovine, Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SOX2
      • ANOP3
      • MCOPS3
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~39 kDa observed. 34.31 kDa (human) and 34.45 (mouse) calculated. Uncharacterized band(s) may appear in some lysates
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse embryonic stem cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected SOX2 in 10 µg of mouse embryonic stem cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Sox2 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Sox2 Antibody Certificates of Analysis

      TitleLot Number
      Anti-SOX2 - 3254559 3254559
      Anti-SOX2 - 3481399 3481399
      Anti-SOX2 - 3587118 3587118
      Anti-SOX2 - 3705707 3705707
      Anti-SOX2 Polyclonal Antibody 3018866
      Anti-Sox2 2477001
      Anti-Sox2 2470969
      Anti-Sox2 3068446
      Anti-Sox2 (rabbit polyclonal) Polyclonal Antibody Q2922255
      Anti-Sox2 - 2387836 2387836

      References

      Reference overviewApplicationSpeciesPub Med ID
      SOX2 reprograms resident astrocytes into neural progenitors in the adult brain.
      Niu, W; Zang, T; Smith, DK; Vue, TY; Zou, Y; Bachoo, R; Johnson, JE; Zhang, CL
      Stem cell reports  4  780-94  2015

      Show Abstract
      25921813 25921813
      Olig1 function is required for oligodendrocyte differentiation in the mouse brain.
      Dai, J; Bercury, KK; Ahrendsen, JT; Macklin, WB
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  4386-402  2015

      Show Abstract
      25762682 25762682
      V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits.
      Pino-Barrio, MJ; García-García, E; Menéndez, P; Martínez-Serrano, A
      PloS one  10  e0118499  2015

      Show Abstract
      25764185 25764185
      Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach.
      Debowski, K; Warthemann, R; Lentes, J; Salinas-Riester, G; Dressel, R; Langenstroth, D; Gromoll, J; Sasaki, E; Behr, R
      PloS one  10  e0118424  2015

      Show Abstract
      Immunofluorescence25785453 25785453
      The temporal expression pattern of alpha-synuclein modulates olfactory neurogenesis in transgenic mice.
      Schreglmann, SR; Regensburger, M; Rockenstein, E; Masliah, E; Xiang, W; Winkler, J; Winner, B
      PloS one  10  e0126261  2015

      Show Abstract
      25961568 25961568
      Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung.
      Galvis, LA; Holik, AZ; Short, KM; Pasquet, J; Lun, AT; Blewitt, ME; Smyth, IM; Ritchie, ME; Asselin-Labat, ML
      Development (Cambridge, England)  142  1458-69  2015

      Show Abstract
      25790853 25790853
      Zebrafish adult-derived hypothalamic neurospheres generate gonadotropin-releasing hormone (GnRH) neurons.
      Cortés-Campos, C; Letelier, J; Ceriani, R; Whitlock, KE
      Biology open  4  1077-86  2015

      Show Abstract
      26209533 26209533
      Periostin secreted by glioblastoma stem cells recruits M2 tumour-associated macrophages and promotes malignant growth.
      Zhou, W; Ke, SQ; Huang, Z; Flavahan, W; Fang, X; Paul, J; Wu, L; Sloan, AE; McLendon, RE; Li, X; Rich, JN; Bao, S
      Nature cell biology  17  170-82  2015

      Show Abstract
      25580734 25580734
      Properties of neurons derived from induced pluripotent stem cells of Gaucher disease type 2 patient fibroblasts: potential role in neuropathology.
      Sun, Y; Florer, J; Mayhew, CN; Jia, Z; Zhao, Z; Xu, K; Ran, H; Liou, B; Zhang, W; Setchell, KD; Gu, J; Grabowski, GA
      PloS one  10  e0118771  2015

      Show Abstract
      Immunofluorescence25822147 25822147
      In vivo reprogrammed pluripotent stem cells from teratomas share analogous properties with their in vitro counterparts.
      Choi, HW; Kim, JS; Hong, YJ; Song, H; Seo, HG; Do, JT
      Scientific reports  5  13559  2015

      Show Abstract
      26315499 26315499

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies