Millipore Sigma Vibrant Logo

AB5603A4 Anti-Sox2, Alexa Fluor® 488 Conjugate Antibody

View Products on Sigmaaldrich.com
AB5603A4
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue NumberAB5603A4
      DescriptionAnti-Sox2, Alexa Fluor® 488 Conjugate Antibody
      Alternate Names
      • Transcription factor SOX-2
      Background InformationSOX2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Undifferentiated ReNcell VM neural progenitor cells
      PresentationPurified rabbit polyclonal conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationDetect Sox2 using this Anti-Sox2, Alexa Fluor 488 Conjugate Antibody validated for use in ICC.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenSynthetic peptide from human Sox2.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
      Gene Symbol
      • SOX2
      • Sox2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Can form ternary complexes with OCT-1 or OCT-3 but acts as a transcriptional activator of FGF-4 enhancer DNA sequences only in conjunction with OCT-3 (By similarity).

      SIZE: 317 amino acids; 34310 Da

      SUBCELLULAR LOCATION: Nucleus.

      DISEASE: Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. MCOPS3 is characterized by the association of microphthalmia or clinical anophthalmia with anomalies such as microcephaly, short stature, hypogonadotropic hypogonadism, esophageal atresia and neurologic manifestations.

      SIMILARITY: Contains 1 HMG box DNA-binding domain.
      Molecular WeightThe unconjugated antibody (AB5603) tested on western blot detects Sox2 in whole cell or nuclear extracts as a protein band at 34 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in undifferentiated ReNcell VM neural progenitor cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sox2 in undifferentiated ReNcell VM neural progenitor cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Sox2, Alexa Fluor® 488 Conjugate Antibody Certificates of Analysis

      TitleLot Number
      Anti-Sox2, Alexa Fluor#174; 488 Conjugate - 2090483 2090483
      Anti-Sox2, Alexa Fluor#174; 488 Conjugate - 2234223 2234223
      Anti-Sox2, Alexa Fluor#174; 488 Conjugate - Q1988593 Q1988593
      Anti-Sox2, Alexa Fluor® 488 -2767711 2767711
      Anti-Sox2, Alexa Fluor® 488 Conjugate 2966323
      Anti-Sox2, Alexa Fluor® 488 Conjugate 2846369
      Anti-Sox2, Alexa Fluor® 488 Conjugate - 2445030 2445030
      Anti-Sox2, Alexa Fluor® 488 Conjugate - 2281683 2281683
      Anti-Sox2, Alexa Fluor® 488 Conjugate - 3275055 3275055
      Anti-Sox2, Alexa Fluor® 488 Conjugate - 3326694 3326694

      References

      Reference overviewPub Med ID
      CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice.
      Haus, DL; Nguyen, HX; Gold, EM; Kamei, N; Perez, H; Moore, HD; Anderson, AJ; Cummings, BJ
      Stem cell research  13  214-26  2014

      Show Abstract
      25082219 25082219

      Related Products & Applications

      Related Products

      Catalog Number Description  
      17-656 ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set Show Pricing & Availability
      AB5603 Anti-Sox2 Antibody Show Pricing & Availability
      AB5603-AF647 Anti-Sox2 Antibody, Alexa Fluor® 647 Conjugate Show Pricing & Availability
      FCMAB112F Anti-SOX-2 Antibody, clone 6G1.2, FITC conjugate Show Pricing & Availability
      MAB4343 Anti-SOX-2 Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies