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AB15766 Anti-Sox1 Antibody

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AB15766
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IHCRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB15766
      ReplacesAB5768
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Sox1 Antibody
      References
      Product Information
      FormatPurified
      PresentationAffinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Sox1 Antibody detects level of Sox1 & has been published & validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 1:200-1:1,000 using ECL. Immunohistochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from human Sox1.
      HostRabbit
      SpecificityRecognizes Sox-1, a high mobility group (HMG) transcription factor.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman and rodent. Reactivity with other species has not been confirmed.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development.
      Gene Symbol
      • SOX1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O00570 # Transcriptional activator (By similarity).
      SIZE: 387 amino acids; 38856 Da
      SUBCELLULAR LOCATION: Nucleus (Probable).
      TISSUE SPECIFICITY: Mainly in the developing central nervous system.
      SIMILARITY: SwissProt: O00570 ## Contains 1 HMG box DNA-binding domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

      PREPARATION AND USE:

      To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

      The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

      For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Sox1 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Sox1 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-Sox-1 2464929
      RABBIT ANTI-Sox-1 3167116
      RABBIT ANTI-Sox-1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2109500 2109500
      RABBIT ANTI-Sox-1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2122437 2122437
      RABBIT ANTI-Sox-1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2397057 2397057
      RABBIT ANTI-Sox-1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2458944 2458944
      RABBIT ANTI-Sox-1 - 3246210 3246210
      RABBIT ANTI-Sox-1 - 3574582 3574582
      RABBIT ANTI-Sox-1 -2701541 2701541
      RABBIT ANTI-Sox-1 -2782374 2782374

      References

      Reference overviewApplicationSpeciesPub Med ID
      Cells with intense EGFR staining and a high nuclear to cytoplasmic ratio are specific for infiltrative glioma: a useful marker in neuropathological practice.
      Burel-Vandenbos, F; Turchi, L; Benchetrit, M; Fontas, E; Pedeutour, Z; Rigau, V; Almairac, F; Ambrosetti, D; Michiels, JF; Virolle, T
      Neuro-oncology  15  1278-88  2013

      Show Abstract
      Immunofluorescence23935154 23935154
      Signaling defects in iPSC-derived fragile X premutation neurons.
      Liu, J; Koscielska, KA; Cao, Z; Hulsizer, S; Grace, N; Mitchell, G; Nacey, C; Githinji, J; McGee, J; Garcia-Arocena, D; Hagerman, RJ; Nolta, J; Pessah, IN; Hagerman, PJ
      Human molecular genetics  21  3795-805  2012

      Show Abstract
      Western Blotting22641815 22641815
      A high-throughput screen for Wnt/β-catenin signaling pathway modulators in human iPSC-derived neural progenitors.
      Zhao, WN; Cheng, C; Theriault, KM; Sheridan, SD; Tsai, LH; Haggarty, SJ
      Journal of biomolecular screening  17  1252-63  2012

      Show Abstract
      22923789 22923789
      Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage.
      Mak, SK; Huang, YA; Iranmanesh, S; Vangipuram, M; Sundararajan, R; Nguyen, L; Langston, JW; Schüle, B
      Stem cells international  2012  140427  2012

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)22567022 22567022
      Guided migration of neural stem cells derived from human embryonic stem cells by an electric field.
      Feng, JF; Liu, J; Zhang, XZ; Zhang, L; Jiang, JY; Nolta, J; Zhao, M
      Stem cells (Dayton, Ohio)  30  349-55  2012

      Show Abstract
      22076946 22076946
      Epigenetic characterization of the FMR1 gene and aberrant neurodevelopment in human induced pluripotent stem cell models of fragile X syndrome.
      Sheridan, SD; Theriault, KM; Reis, SA; Zhou, F; Madison, JM; Daheron, L; Loring, JF; Haggarty, SJ
      PloS one  6  e26203  2011

      Show Abstract
      Western Blotting22022567 22022567
      Novel autogenic feeders derived from human embryonic stem cells (hESCs) support an undifferentiated status of hESCs in xeno-free culture conditions.
      Chen, Hsin-Fu, et al.
      Hum. Reprod., 24: 1114-25 (2009)  2009

      Show Abstract
      ImmunofluorescenceHuman19202140 19202140

      Data Sheet

      Title
      RABBIT ANTI-Sox-1 AFFINITY PURIFIED POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies