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AB5210 Anti-Sodium Channel Antibody, Voltage Gated, Pan (SP19 Segment)

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AB5210
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5210
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Sodium Channel Antibody, Voltage Gated, Pan (SP19 Segment)
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationThis Sodium Channel antibody is validated for use in IHC & WB for the detection of the Sodium Channel protein.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesAll procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

      Western blot: 1:200 using ECL on newborn rat brain membranes.

      Immunohistochemistry on mouse brain sections (frozen) and mouse spinal cord sections (paraffin).

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified peptide corresponding to amino acids 1501-1518 in the alpha subunit (amino acids 1501-1518) of rat type I voltage-gated sodium channel (VGSC) (Accession P04774).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes alpha subunits of VGSC. Does not cross react with any other sodium channel antigens tested so far.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe vertebrate sodium channel is a voltage-gated ion channel essential for the generation and propagation of action potentials, chiefly in nerve and muscle. Voltage-sensitive sodium channels are heteromeric complexes consisting of a large central pore-forming glycosylated alpha subunit and 2 smaller auxiliary beta subunits. Functional studies have indicated that the transmembrane alpha subunit of the brain sodium channels is sufficient for expression of functional sodium channels (Goldin et al., 1986 [PubMed 2429308]; Isom, 2002 [PubMed 11779698]).[supplied by OMIM]
      Gene Symbol
      • SCN1A
      • SCN1
      • HBSCI
      • Nav1.1
      • FEB3
      • NAC1
      • SMEI
      • GEFSP2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35498 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
      SIZE: 2009 amino acids; 228972 Da
      SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      DOMAIN: SwissProt: P35498 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
      DISEASE: SwissProt: P35498 # Defects in SCN1A are the cause of generalized epilepsy with febrile seizures plus type 2 (GEFS+2) [MIM:604233]. This autosomal dominant disorder is characterized by febrile seizures in children and afebrile seizures in adults. Penetrance is incomplete and a large intrafamilial variability of the phenotype is observed. & Defects in SCN1A are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a severe form of generalized epilepsy with febrile seizures. It is a rare disorder characterized by normal development before onset, seizures beginning in the first year of life in the form of generalized or unilateral febrile clonic seizures, secondary appearance of myoclonic seizures, and occasionally partial seizures. It is associated with ataxia, slowed psychomotor development, and mental decline. & Defects in SCN1A are the cause of familial hemiplegic migraine 3 (FHM3) [MIM:609634]. FHM3 is an autosomal dominant severe subtype of migraine with aura characterized by some degree of hemiparesis during the attacks. The episodes are associated with variable features of nausea, vomiting, photophobia and phonophobia. Age at onset ranges from 6 to 15 years. Some patients may manifest seizures during infancy. & Defects in SCN1A are the cause of familial febrile convulsions 3 (FEB3) [MIM:604403]; also known as familial febrile seizures 3. Febrile convulsions affect 5-12% of infants and children up to 6 years of age. There is epidemiological evidence that febrile seizures are associated with subsequent afebrile and unprovoked seizures in 2% to 7% of patients. Inheritance pattern is autosomal dominant.
      SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Sodium Channel Antibody, Voltage Gated, Pan (SP19 Segment) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Sodium Channel Antibody, Voltage Gated, Pan (SP19 Segment) Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2115529 2115529
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2139982 2139982
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2365937 2365937
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav - 3173833 3173833
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav - 3257097 3257097
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav - 3439800 3439800
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav - 3863409 3863409
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav -2636724 2636724
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav -2716590 2716590
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav -2734775 2734775

      References

      Reference overviewApplicationPub Med ID
      Effective contractile response to voltage-gated Na+ channels revealed by a channel activator.
      Ho, WS; Davis, AJ; Chadha, PS; Greenwood, IA
      American journal of physiology. Cell physiology  304  C739-47  2013

      Show Abstract
      Immunofluorescence23364266 23364266
      Putative excitatory and putative inhibitory inputs are localised in different dendritic domains in a Drosophila flight motoneuron.
      Kuehn, C; Duch, C
      The European journal of neuroscience  37  860-75  2013

      Show Abstract
      23279094 23279094
      Colocalization of hyperpolarization-activated, cyclic nucleotide-gated channel subunits in rat retinal ganglion cells.
      Stradleigh, TW; Ogata, G; Partida, GJ; Oi, H; Greenberg, KP; Krempely, KS; Ishida, AT
      The Journal of comparative neurology  519  2546-73  2011

      Show Abstract
      21456027 21456027

      Data Sheet

      Title
      RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, Nav AFFINITY PURIFIED POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies