DP02 Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416)

DP02
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Sv40 Infected Cells M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      DP02-200UG
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      Discontinued
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          Glass bottle 200 μg
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          Description
          OverviewRecognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells.
          Catalogue NumberDP02
          Brand Family Calbiochem®
          References
          ReferencesDeCaprio, J.A., et al. 1988. Cell 54, 275.
          Whyte, P., et al. 1988. Nature 334, 124.
          Mitchell, P.J., et al. 1987. Cell 50, 847.
          Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
          Simanis, V. and Lane, D.P. 1985. Virol. 144, 88.
          Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379.
          Sarnow, P., et al. 1982. Cell 28, 387.
          Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
          Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
          Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
          Tooze, J. 1973. Cold Spring Harbor, New York.
          Tegtmeyer, P. 1972. J. Virol. 10, 591.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Negative control3T3 cells
          Positive controlSV80 or SV-T2 cells
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Application ReferencesImmunoblotting Radhakrishnan, S., et al. 2004. J. Virol. 78, 7264. Ricciardiello, L., et al. 2003. Cancer Res. 63, 7256. Original Clone Harlow, E., et al. 1981. J. Virol. 39, 861. Paraffin Sections Sabatier, J., et al. 2005. J. Clin. Pathol. 58, 429. Del Valle, L., et al. 2004. J. Virol. 78, 3462. Del Valle, L., et al. 2001. Cancer Res. 61, 4287. Masumori, N., et al. 2001. Cancer Res. 61, 2239.
          Key Applications Paraffin Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Application NotesAffinity Purification (use Cat. No. DP02A)
          Immunoblotting (1-5 µg/ml, see application references)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)
          Paraffin Sections (see application references)
          Application CommentsThe agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpurified SV40 large T-antigen
          EpitopeWithin amino acids 83-128
          ClonePAb416
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Sv40 Infected Cells
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Certificates of Analysis

          TitleLot Number
          DP02

          References

          Reference overview
          DeCaprio, J.A., et al. 1988. Cell 54, 275.
          Whyte, P., et al. 1988. Nature 334, 124.
          Mitchell, P.J., et al. 1987. Cell 50, 847.
          Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
          Simanis, V. and Lane, D.P. 1985. Virol. 144, 88.
          Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379.
          Sarnow, P., et al. 1982. Cell 28, 387.
          Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
          Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
          Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
          Tooze, J. 1973. Cold Spring Harbor, New York.
          Tegtmeyer, P. 1972. J. Virol. 10, 591.

          Citations

          Title
        • Sujatha Radhakrishman, et al. (2004) Intracellular Approach for Blocking JC Virus Gene Expression by Using RNA Interference During Viral Infection. Journal of Virology 78, 7264-7269.
        • Luigi Ricciardiello, et al. (2003) Induction of chromosomal Instability in Colonic Cell by the Human Polyomavirus JC Virus. Cancer Research 63, 7256-7262.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-July-2007 JSW
          ApplicationAffinity Purification (use Cat. No. DP02A)
          Immunoblotting (1-5 µg/ml, see application references)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)
          Paraffin Sections (see application references)
          DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen.
          BackgroundSV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
          HostMouse
          Immunogenpurified SV40 large T-antigen
          EpitopeWithin amino acids 83-128
          ClonePAb416
          IsotypeIgG2a
          SpeciesSV40 infected cells
          Positive controlSV80 or SV-T2 cells
          Negative control3T3 cells
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThe agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesDeCaprio, J.A., et al. 1988. Cell 54, 275.
          Whyte, P., et al. 1988. Nature 334, 124.
          Mitchell, P.J., et al. 1987. Cell 50, 847.
          Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
          Simanis, V. and Lane, D.P. 1985. Virol. 144, 88.
          Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379.
          Sarnow, P., et al. 1982. Cell 28, 387.
          Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
          Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
          Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
          Tooze, J. 1973. Cold Spring Harbor, New York.
          Tegtmeyer, P. 1972. J. Virol. 10, 591.
          Citation
        • Sujatha Radhakrishman, et al. (2004) Intracellular Approach for Blocking JC Virus Gene Expression by Using RNA Interference During Viral Infection. Journal of Virology 78, 7264-7269.
        • Luigi Ricciardiello, et al. (2003) Induction of chromosomal Instability in Colonic Cell by the Human Polyomavirus JC Virus. Cancer Research 63, 7256-7262.
        • Application referencesImmunoblotting Radhakrishnan, S., et al. 2004. J. Virol. 78, 7264. Ricciardiello, L., et al. 2003. Cancer Res. 63, 7256. Original Clone Harlow, E., et al. 1981. J. Virol. 39, 861. Paraffin Sections Sabatier, J., et al. 2005. J. Clin. Pathol. 58, 429. Del Valle, L., et al. 2004. J. Virol. 78, 3462. Del Valle, L., et al. 2001. Cancer Res. 61, 4287. Masumori, N., et al. 2001. Cancer Res. 61, 2239.

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies