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DP01 Anti-SV40 T Antigen (Ab-1) Mouse mAb (PAb419)

DP01
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Sv40 Infected Cells M Monoclonal Antibody

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DP01-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~94 kDa SV40 large T antigen and the ~21 kDa SV40 small T antigen.
      Catalogue NumberDP01
      Brand Family Calbiochem®
      References
      ReferencesDeCaprio, J.A., et al.1988. Cell 54, 275.
      Whyte, P., et al. 1988 Nature 334, 124.
      Mitchell, P.J., et al.1987 Cell 50, 847.
      Dixon, R.A.F. and Nathans, D., 1985 J. Virol. 53, 1001.
      Simanis, V. and Lane, D.P., 1985 Virol. 144, 88.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J.1973. Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10, 591.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Positive controlSV80 or SV-T2 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Harlow, E., et al. 1981 J. Virol. 39, 861. Immunoblotting Goolsby, C.L., et al. 1991. Cytometry 12, 748.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (see application references)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenthe N-terminal domain of SV40 large and small T-antigen
      ClonePAb419
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Sv40 Infected Cells
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-SV40 T Antigen (Ab-1) Mouse mAb (PAb419) Certificates of Analysis

      TitleLot Number
      DP01

      References

      Reference overview
      DeCaprio, J.A., et al.1988. Cell 54, 275.
      Whyte, P., et al. 1988 Nature 334, 124.
      Mitchell, P.J., et al.1987 Cell 50, 847.
      Dixon, R.A.F. and Nathans, D., 1985 J. Virol. 53, 1001.
      Simanis, V. and Lane, D.P., 1985 Virol. 144, 88.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J.1973. Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10, 591.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      ApplicationImmunoblotting (see application references)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen and the ~20.5 kDa SV40 small T antigen.
      BackgroundSV40 T Ag (Ab-1) is a mouse monoclonal antibody with specificity for antigenic determinants localized to the amino terminal domain of the SV40 large T antigen. The sequences recognized by this reagent are also present on SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
      HostMouse
      Immunogenthe N-terminal domain of SV40 large and small T-antigen
      ClonePAb419
      IsotypeIgG2a
      SpeciesSV40 infected cells
      Positive controlSV80 or SV-T2 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesDeCaprio, J.A., et al.1988. Cell 54, 275.
      Whyte, P., et al. 1988 Nature 334, 124.
      Mitchell, P.J., et al.1987 Cell 50, 847.
      Dixon, R.A.F. and Nathans, D., 1985 J. Virol. 53, 1001.
      Simanis, V. and Lane, D.P., 1985 Virol. 144, 88.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J.1973. Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10, 591.
      Application referencesOriginal Clone Harlow, E., et al. 1981 J. Virol. 39, 861. Immunoblotting Goolsby, C.L., et al. 1991. Cytometry 12, 748.