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MAB4301-25UG Anti-SSEA-1, clone MC-480

MAB4301-25UG
25 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, IF, IHC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4301-25UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-SSEA-1, clone MC-480
      Alternate Names
      • SSEA-1
      • CD15
      • Lewis X
      • 3-FAL
      Background InformationSSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
      References
      Product Information
      FormatPurified
      Control
      • P19 mouse embryonic carcinoma cells
      PresentationPurified  Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide mouse monoclonal IgM in buffer containing 0.05M 
      Quality LevelMQ100
      Applications
      ApplicationAnti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was tested on IH.

      Immunoprecipitation:
      A previous lot of this antibody was tested on IP.

      Immunofluorescence:
      A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.

      Flow Cytometry:
      A starting range of 10-20 µg/mL is suggested.

      Positive mouse EC lines include:
      F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
      Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenF9 tetracarcinoma stem cells (X-irradiated).
      CloneMC-480
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThis antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
      IsotypeIgM
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryFUT4 catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). It has been detected in human embryos (5-10 weeks) suggesting a role in development.
      Gene Symbol
      • FUT4
      • ELFT
      • FCT3A
      • Fuc-TIV
      • 1,3fucosyltransferase
      • FUC-TIV
      • FucT-IV
      • CD15
      • Alpha-
      • EC 2.4.1.-
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P22083 # May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
      SIZE: 405 amino acids; 45570 Da
      SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
      SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      • Neural Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight220 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceTested
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications