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MABC785 Anti-SOX9 Antibody, clone 4B7.1

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MABC785
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC785
      ReplacesMABC672
      DescriptionAnti-SOX9 Antibody, clone 4B7.1
      Alternate Names
      • Transcription factor SOX-9
      • Sex-determining region Y-box 9 protein
      • SRY-related HMG-box gene 9
      Background InformationTranscription factor SOX-9 (UniProt P48436; also known as Sex-determining region Y-box 9 protein, SRY-related HMG-box gene 9) is encoded by the SOX9 (also known as CMD1, CMPD1, SRA1) gene (Gene ID 6662) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences encoding for the HMG-box domains that mediate sequ+D11ence-specific DNA binding. SOX genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at at least 40 genomic loci and at least 30 Sox genes have been identified. SOX-9 is a key regulator of developmental processes, including male sex determination, chondrogenesis, neurogenesis, and neural crest development. SOX-9 up-regulation is reported in cancers of various origins, including breast, colon, prostate, and lung, where its expression correlates with malignancy and patient survival. Cellular SOX9 mRNA is shown to be targeted and negatively regulated+D11 by miR-32, and the cellular miR-32 level is reported to be significantly lower in non-small cell lung cancer (NSCLC) compared with noncancerous lung tissues.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect SOX9 using this mouse monoclonal Anti-SOX9, clone 4B7.1 Antibody, Cat. No. MABC785, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SOX9 in human liver cancer and small intestine tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human SOX9 C-terminal end sequence.
      Clone4B7.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4B7.1 epitope lies within the highly conserved C-terminal end region of SOX9.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Chicken, Mouse, Non-Human Primate, Porcine, Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SOX9
      • CMD1
      • CMPD1
      • SRA1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~69 kDa observed. 54.85/56.14/56.08/56.12/56.12 kDa (chicken/human/mouse/porcine/rat) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SOX9 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications