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MABN834 Anti-SOD1 Antibody (ALS mutant), clone MS785

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MABN834
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ICC, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN834
      DescriptionAnti-SOD1 Antibody (ALS mutant), clone MS785
      Alternate Names
      • Superoxide dismutase [Cu-Zn]
      • Superoxide dismutase 1
      • hSod1
      • SOD1
      Background InformationSOD1 or Superoxide dismutase 1 is a critical enzyme for free radical metabolism and serves as a major detoxifier in cellular metabolism. SOD1 mutations are associated with disease and in particular with Amyotrophic lateral sclerosis 1, or ALS, often referred to as “Lou Gehrig’s Disease.” Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that is characterized by the selective loss of upper and lower motor neurons. There are >100 mutations in SOD1 in humans but not all appear to be pathogenic. The clone MS785 is a monoclonal antibody generated against the Derlin-1-binding region of SOD1 (DBR), and it distinguished most ALS-causative SOD1 mutants from both wild-type and nontoxic mutants. Moreover, MS785 recognized endogenous SOD1 in B lymphocytes derived from 14 ALS patients carrying SOD1 mutations but not from 11 healthy controls, thus MS785 appears to selectively recognize all Derlin-1–interactive SOD1 mutants but not WT SOD1.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Anti-SOD1 Antibody (ALS mutant ), clone MS785 using this Anti-SOD1 Antibody (ALS mutant ), clone MS785 validated for use in Western Blotting
      and Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated SOD1 in transfected HEK293 cells (Fujisawa, T., et al. (2012). Ann Neurol. 72: 739–749).
      Western Blotting Analysis: A representative lot SOD1 in transfected HEK293 cells (Fujisawa, T., et al. (2012). Ann Neurol. 72: 739–749).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated SOD1 in transfected HEK293 cells (Homma, K., et al. (2013). Ann Neurol. Cell. 52:1-12).
      Immunocytochemistry (ICC): A representative lot of this antibody detected SOD1-G93A in HEK293 cells overexpressing G93A, but not the wild-type SOD1. (Fujisawa, T., et al. 2015. Neurobiol. Dis. 82; 486-487).
      Immunohistochemistry (IHC): A representative lot of this antibody detected SOD1-G93A in the anterior horns of spinal cord tissue sections of SOD1G93A-transgenic mice, but not non-transgenic mice. ((Fujisawa, T., et al. 2015. Neurobiol. Dis. 82; 486-487).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human SOD1.
      EpitopeN-terminus
      CloneMS785
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SOD1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~16 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in transfected HEK293 with SOD1 mutant and HEK293 control lysates.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected SOD1 in 1 µg of transfected HEK293 with SOD1 mutant and HEK293 control lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-SOD1 Antibody (ALS mutant), clone MS785 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SOD1 Antibody (ALS mutant), clone MS785 Certificates of Analysis

      TitleLot Number
      Anti-SOD1 (ALS mutant ), clone MS785 3072838
      Anti-SOD1 (ALS mutant ), clone MS785 - 3167078 3167078
      Anti-SOD1 (ALS mutant ), clone MS785 - 3398035 3398035
      Anti-SOD1 (ALS mutant ), clone MS785 -2831771 2831771
      Anti-SOD1 (ALS mutant ), clone MS785 -Q2514176 Q2514176
      Anti-SOD1 (ALS mutant ), clone MS785 Monoclonal Antibody 2831771A
      Anti-SOD1 (ALS mutant ), clone MS785 Monoclonal Antibody 2938804

      References

      Reference overviewPub Med ID
      Molecular cell
      Homma, K; Fujisawa, T; Tsuburaya, N; Yamaguchi, N; Kadowaki, H; Takeda, K; Nishitoh, H; Matsuzawa, A; Naguro, I; Ichijo, H
      Molecular cell  52  75-86  2013

      Show Abstract
      24076220 24076220
      Annals of neurology
      Fujisawa, T; Homma, K; Yamaguchi, N; Kadowaki, H; Tsuburaya, N; Naguro, I; Matsuzawa, A; Takeda, K; Takahashi, Y; Goto, J; Tsuji, S; Nishitoh, H; Ichijo, H
      Annals of neurology  72  739-49  2012

      Show Abstract
      23280792 23280792

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies