04-1551 Anti-SMRT Antibody, clone 1SM-1E7

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04-1551
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB M Ascites Monoclonal Antibody
      Description
      Catalogue Number04-1551
      DescriptionAnti-SMRT Antibody, clone 1SM-1E7
      Alternate Names
      • CTG repeat protein 26
      • Silencing mediator of retinoic acid and thyroid hormone receptor
      • T3 receptor-associating factor
      • Thyroid-, retinoic-acid-receptor-associated corepressor
      • nuclear receptor co-repressor 2
      • silencing mediator for retinoid and thyroid hormone receptors
      Background InformationThe Silencing mediator of retinoic acid & thyroid hormone receptor protein, commonly called the SMRT protein mediates the transcriptional repression of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription machinery. Consistent with this activity, this protein is known to form a large corepressor complex containing SIN3A/B and histone deacetylases HDAC1 and HDAC2. SMRT is also a component of the N-Cor repressor (nuclear receptor corepressor), a multi-subunit complex minimally composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. SMRT and nuclear receptor corepressor N-CoR are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
      References
      Product Information
      FormatAscites
      Control
      • Human brain lysate
      PresentationUnpurified mouse monoclonal IgG2aκ ascites with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-SMRT Antibody, clone 1SM-1E7 (Mouse Monoclonal Antibody) validated in WB to detect SMRT also known as CTG repeat protein 26, Silencing mediator of retinoic acid & thyroid hormone receptor.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant protein corresponding to human SMRT.
      EpitopeUnknown
      Clone1SM-1E7
      HostMouse
      SpecificityThis antibody recognizes SMRT.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NCOR2
      • CTG26
      • N-CoR2
      • SMAP270
      • SMRT
      • SMRTE
      • SMRTE-tau
      • TNRC14
      • TRAC
      • TRAC-1
      • TRAC1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription.

      SIZE: 2517 amino acids; 274034 Da

      SUBUNIT: Interacts with HDAC7 (By similarity). Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid (TR) and the retinoid acid receptors (RAR) in the absence of ligand, and may stabilize their interaction with TFIIB. The SRMT isoform interacts with HDAC10. Interacts with MINT. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with CBFA2T3.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Ubiquitous. High levels of expression are detected in lung, spleen and brain.

      DOMAIN: SwissProt: Q9Y618 The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor- interacting domains that are divided in two separate interaction domains (ID1 and ID2). & The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.

      SIMILARITY: Belongs to the N-CoR nuclear receptor corepressors family. & Contains 2 SANT domains.
      Molecular Weight~ 275 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human brain lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected SMRT on 10 µg of human brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-SMRT Antibody, clone 1SM-1E7 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SMRT Antibody, clone 1SM-1E7 Certificates of Analysis

      TitleLot Number
      Anti-SMRT, clone 1SM-1E7 - 23922562392256
      Anti-SMRT, clone 1SM-1E7 - 24364262436426
      Anti-SMRT, clone 1SM-1E7 - 19912821991282
      Anti-SMRT, clone 1SM-1E7 - 20747782074778
      Anti-SMRT, clone 1SM-1E7 - 22917112291711
      Anti-SMRT, clone 1SM-1E7 - 25274562527456
      Anti-SMRT, clone 1SM-1E7 - 25378652537865
      Anti-SMRT, clone 1SM-1E7 - 33276083327608
      Anti-SMRT, clone 1SM-1E7 - JBC1771343JBC1771343
      Anti-SMRT, clone 1SM-1E7 - NG1862443NG1862443

      References

      Reference overviewPub Med ID
      Histone deacetylase turnover and recovery in sulforaphane-treated colon cancer cells: competing actions of 14-3-3 and Pin1 in HDAC3/SMRT corepressor complex dissociation/reassembly.
      Rajendran, P; Delage, B; Dashwood, WM; Yu, TW; Wuth, B; Williams, DE; Ho, E; Dashwood, RH
      Molecular cancer  10  68  2011

      Show Abstract
      21624135 21624135

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies