CB1039 Anti-S100A4 Mouse mAb (1F12-1G7)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M M Monoclonal Antibody

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CB1039-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~10 kDa S100A4 protein in HeLa and NIH3T3 cells.
      Catalogue NumberCB1039
      Brand Family Calbiochem®
      SynonymsAnti-S100 Calcium Binding Protein A4
      Application Data
      Detection of human S100A4 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse S100A4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human S100A4 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa cells, NIH3T3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human S100A4 (aa 1-102), expressed as a GST fusion protein
      Clone1F12-1G7
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-S100A4 Mouse mAb (1F12-1G7) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-S100 Calcium Binding Protein A4
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human S100A4 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse S100A4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human S100A4 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouae monoclonal antibody. Recognizes the ~10 kDa S100A4 protein.
      BackgroundThe protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
      HostMouse
      ImmunogenFull-length, recombinant, human S100A4 (aa 1-102), expressed as a GST fusion protein
      Clone1F12-1G7
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlHeLa cells, NIH3T3 cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review