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MAB5316 Anti-Rhodopsin Antibody, clone RET-P1

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MAB5316
500 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5316
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Rhodopsin Antibody, clone RET-P1
      Alternate Names
      • Opsin 2
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL:

        IMR-5 cells, brain or retina.
      PresentationPurified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Rhodopsin using this Anti-Rhodopsin Antibody, clone RET-P1 validated for use in IC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunoblotting: 1 μg/mL

      Immunocytochemistry

      Immunohistochemistry (frozen and formalin/paraffin): 1-2 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenMembrane preparation from adult rat retina.
      CloneRET-P1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with a protein of 39 kDa identified as rhodopsin (opsin). MAB5316 specifically labels the axons and synaptic pedicles of the rods.

      CELLULAR LOCALIZATION:

      Cytoplasmic
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryRetinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness.
      Gene Symbol
      • RHO
      • rhodopsin
      • Opsin-2
      • OPN2
      • Rhodopsin
      • RP4
      • MGC138311
      • MGC138309
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08100 # Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
      SIZE: 348 amino acids; 38893 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Rod shaped photoreceptor cells which mediates vision in dim light.
      PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
      DISEASE: SwissProt: P08100 # Defects in RHO are the cause of retinitis pigmentosa type 4 (RP4) [MIM:180380]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP4 inheritance is autosomal dominant. & Defects in RHO are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. & Defects in RHO are a cause of autosomal dominant congenital stationary night blindness 1 (CSNBAD1) [MIM:610445]; also known as rhodopsin-related congenital stationary night blindness. Congenital stationary night blindness is a nonprogressive retinal disorder characterized by impaired night vision and ocular symptoms such as myopia, hyperopia, nystagmus and reduced visual acuity.
      SIMILARITY: SwissProt: P08100 ## Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Rhodopsin Antibody, clone RET-P1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Rhodopsin Antibody, clone RET-P1 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-RHODOPSIN 3054658
      MOUSE ANTI-RHODOPSIN MONOCLONAL ANTIBODY - 2121742 2121742
      MOUSE ANTI-RHODOPSIN - 2492131 2492131
      MOUSE ANTI-RHODOPSIN - 3184121 3184121
      MOUSE ANTI-RHODOPSIN - 3188689 3188689
      MOUSE ANTI-RHODOPSIN - 3219928 3219928
      MOUSE ANTI-RHODOPSIN - 3288789 3288789
      MOUSE ANTI-RHODOPSIN - 3380383 3380383
      MOUSE ANTI-RHODOPSIN - 3458137 3458137
      MOUSE ANTI-RHODOPSIN - 3483449 3483449

      References

      Reference overviewApplicationSpeciesPub Med ID
      Canine retina has a primate fovea-like bouquet of cone photoreceptors which is affected by inherited macular degenerations.
      Beltran, WA; Cideciyan, AV; Guziewicz, KE; Iwabe, S; Swider, M; Scott, EM; Savina, SV; Ruthel, G; Stefano, F; Zhang, L; Zorger, R; Sumaroka, A; Jacobson, SG; Aguirre, GD
      PloS one  9  e90390  2014

      Show Abstract
      24599007 24599007
      Vibratome sectioning mouse retina to prepare photoreceptor cultures.
      Clérin, E; Yang, Y; Forster, V; Fontaine, V; Sahel, JA; Léveillard, T
      Journal of visualized experiments : JoVE  2014

      Show Abstract
      25548881 25548881
      Structural and functional effects of hemiretinal endodiathermy axotomy in cynomolgus macaques.
      Dashek, RJ; Kim, CB; Rasmussen, CA; Hennes-Beean, EA; Ver Hoeve, JN; Nork, TM
      Investigative ophthalmology & visual science  54  3479-92  2013

      Show Abstract
      Immunohistochemistry23620427 23620427
      Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa.
      Tucker, BA; Mullins, RF; Streb, LM; Anfinson, K; Eyestone, ME; Kaalberg, E; Riker, MJ; Drack, AV; Braun, TA; Stone, EM
      eLife  2  e00824  2013

      Show Abstract
      23991284 23991284
      Functional and anatomic consequences of subretinal dosing in the cynomolgus macaque.
      Nork, TM; Murphy, CJ; Kim, CB; Ver Hoeve, JN; Rasmussen, CA; Miller, PE; Wabers, HD; Neider, MW; Dubielzig, RR; McCulloh, RJ; Christian, BJ
      Archives of ophthalmology (Chicago, Ill. : 1960)  130  65-75  2012

      Show Abstract
      21911651 21911651
      Localization of acetylcholine-related molecules in the retina: implication of the communication from photoreceptor to retinal pigment epithelium.
      Matsumoto, H; Shibasaki, K; Uchigashima, M; Koizumi, A; Kurachi, M; Moriwaki, Y; Misawa, H; Kawashima, K; Watanabe, M; Kishi, S; Ishizaki, Y
      PloS one  7  e42841  2012

      Show Abstract
      22880119 22880119
      Localization of Usher 1 proteins to the photoreceptor calyceal processes, which are absent from mice.
      Sahly, I; Dufour, E; Schietroma, C; Michel, V; Bahloul, A; Perfettini, I; Pepermans, E; Estivalet, A; Carette, D; Aghaie, A; Ebermann, I; Lelli, A; Iribarne, M; Hardelin, JP; Weil, D; Sahel, JA; El-Amraoui, A; Petit, C
      The Journal of cell biology  199  381-99  2012

      Show Abstract
      23045546 23045546
      Longitudinal assessment of retinal structure and function reveals a rod-cone degeneration in a guinea pig Model initially presented as night blind.
      Racine J, Joly S, Lachapelle P
      Documenta ophthalmologica Advances in ophthalmology  2011

      Show Abstract
      21656001 21656001
      Two transcription factors can direct three photoreceptor outcomes from rod precursor cells in mouse retinal development.
      Ng, L; Lu, A; Swaroop, A; Sharlin, DS; Swaroop, A; Forrest, D
      The Journal of neuroscience : the official journal of the Society for Neuroscience  31  11118-25  2011

      Show Abstract
      21813673 21813673
      The F-BAR Protein Rapostlin Regulates Dendritic Spine Formation in Hippocampal Neurons.
      Wakita Y, Kakimoto T, Katoh H, Negishi M
      The Journal of biological chemistry  286  32672-83. Epub 2011 Jul 15.  2011

      Show Abstract
      21768103 21768103

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies