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MABN15 Anti-Rhodopsin Antibody, clone 4D2

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, F, MIHC, WB, ICC, ELISAMPurifiedMonoclonal Antibody
      Catalogue NumberMABN15
      DescriptionAnti-Rhodopsin Antibody, clone 4D2
      Alternate Names
      • rhodopsin
      • rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant)
      • retinitis pigmentosa 4, autosomal dominant
      • opsin 2, rod pigment
      Background InformationRhodopsin is a multi-pass, G-protein-coupled membrane receptor protein belonging to the G-protein coupled receptor 1 family. Found within rod photoreceptor cells, rhodopsin is activated by light photons in order for the visual transduction cascade to initialize. Rhodopsin is necessary to the forming of images in low light and for the viability of photoreceptor cells after birth. Mutations in rhodopsin expression are the most widely known cause of the human disease retinitis pigmentosa (RP) resulting in gradual loss of vision. At present, there are over 100 known rhodopsin mutations.
      Product Information
      • Rat eye tissue
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-Rhodopsin Antibody, clone 4D2 is an antibody against Rhodopsin for use in IH, WB, IC, ELISA.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      • Immunocytochemistry
      • ELISA
      Application NotesWestern Blot Analysis: A previous lot of this antibody detected Rhodopsin by WB in goldfish retina lysate, as reported by Knight K, et al.

      Immunocytochemistry: A previous lot of this antibody detected Rhodopsin by ICC in goldfish retina tissue, as reported by Knight K, et al

      Immunohistochemistry Analysis: 1:500 dilution of this antibody has been shown to detect Rhodopsin in human eye tissue.

      ELISA: Specificity of this antibody, from a different lot, was validated in a competitive ELISA, as reported by Laird D, et al.
      Biological Information
      ImmunogenRat rod outer segments corresponding to rat Rhodopsin at the N-terminus.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes Rhodopsin at the N-terminus.
      Species Reactivity
      • Human
      • Rat
      • Fish
      • Mouse
      Species Reactivity NoteDemonstrated to react with human and rat. Predicted to react with mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryVisual pigment protein of rod photoreceptors involved in G-protein coupled receptor mediated signaling and phototransduction [RGD].
      Gene Symbol
      • OPN2
      • RHO
      • RP4
      • CSNBAD
      • Opsin-2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal (By similarity).

      SUBUNIT STRUCTURE: Homodimer (By similarity).

      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia (By similarity).

      TISSUE SPECIFICITY: Rod shaped photoreceptor cells which mediates vision in dim light.

      PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.

      Contains one covalently linked retinal chromophore (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

      BIOPHYSICOCHEMICAL PROPERTIES: Absorption: Abs(max)=495 nm
      Molecular WeightExpected at ~ 39 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat eye tissue.

      Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Rhodopsin in rat eye tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-Rhodopsin Antibody, clone 4D2 Certificates of Analysis

      TitleLot Number
      Anti-Rhodopsin, clone 4D2 - 2438709 2438709
      Anti-Rhodopsin, clone 4D2 - 2038649 2038649
      Anti-Rhodopsin, clone 4D2 - 2074782 2074782
      Anti-Rhodopsin, clone 4D2 - 2146005 2146005
      Anti-Rhodopsin, clone 4D2 - 2203089 2203089
      Anti-Rhodopsin, clone 4D2 - 2272161 2272161
      Anti-Rhodopsin, clone 4D2 - 2299602 2299602
      Anti-Rhodopsin, clone 4D2 - 2332589 2332589
      Anti-Rhodopsin, clone 4D2 - 2887591 2887591
      Anti-Rhodopsin, clone 4D2 - 3185627 3185627


      Reference overviewPub Med ID
      The Effect of Transient Local Anti-inflammatory Treatment on the Survival of Pig Retinal Progenitor Cell Allotransplants.
      Abud, M; Baranov, P; Hicks, C; Patel, S; Lieppman, B; Regatieri, C; Sinden, J; Isaac, D; Avila, M; Young, M
      Translational vision science & technology  4  6  2015

      Show Abstract
      26425402 26425402
      Loss of citron kinase affects a subset of progenitor cells that alters late but not early neurogenesis in the developing rat retina.
      Karunakaran, DK; Chhaya, N; Lemoine, C; Congdon, S; Black, A; Kanadia, R
      Investigative ophthalmology & visual science  56  787-98  2015

      Show Abstract
      25593024 25593024
      Localization of complement factor H gene expression and protein distribution in the mouse outer retina.
      Smit-McBride, Z; Oltjen, SL; Radu, RA; Estep, J; Nguyen, AT; Gong, Q; Hjelmeland, LM
      Molecular vision  21  110-23  2015

      Show Abstract
      25684976 25684976
      Repair of rhodopsin mRNA by spliceosome-mediated RNA trans-splicing: a new approach for autosomal dominant retinitis pigmentosa.
      Berger, A; Lorain, S; Joséphine, C; Desrosiers, M; Peccate, C; Voit, T; Garcia, L; Sahel, JA; Bemelmans, AP
      Molecular therapy : the journal of the American Society of Gene Therapy  23  918-30  2015

      Show Abstract
      25619725 25619725
      Long-term retinal cone survival and delayed alteration of the cone mosaic in a transgenic mouse model of stargardt-like dystrophy (STGD3).
      Kuny, S; Filion, MA; Suh, M; Gaillard, F; Sauvé, Y
      Investigative ophthalmology & visual science  55  424-39  2014

      Show Abstract
      24334447 24334447
      Prenatal hypoxia is associated with long-term retinal dysfunction in rats.
      Bourque, SL; Kuny, S; Reyes, LM; Davidge, ST; Sauvé, Y
      PloS one  8  e61861  2013

      Show Abstract
      23610595 23610595
      Differential gene expression in eyecup and retina of a mouse model of Stargardt-like macular dystrophy (STGD3).
      Kuny, S; Gaillard, F; Sauvé, Y
      Investigative ophthalmology & visual science  53  664-75  2012

      Show Abstract
      22199241 22199241