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MAB5428 Anti-Retinal Pigment Epithelium 65 Antibody

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100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, XnELISA, IHC, IP, WBMPurifiedMonoclonal Antibody
      Catalogue NumberMAB5428
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Retinal Pigment Epithelium 65 Antibody
      Alternate Names
      • RPE65
      Product Information
      • Eye
      PresentationPurified immunoglobulin. Liquid in PBS. Contains no preservative.
      Quality LevelMQ100
      ApplicationAnti-Retinal Pigment Epithelium 65 Antibody detects level of Retinal Pigment Epithelium 65 & has been published & validated for use in ELISA, IH, IP & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: 1:5,000-1:20,000 on bovine RPE membranes using ECL. Suggested dilution buffer is TBS containing 10% calf serum, 0.25% T-20, 1M D-glucose with 10% glycerol. Suggested blocking buffer is TBS containing 2% BSA and 0.5% Tween 20. Preferred gel percentage is 10%.

      Immunohistochemistry on frozen tissue sections: 1:250-1:500

      Immunoprecipitation: 20 μg of antibody in a reaction volume of 500 μL.

      Immunoaffinity purification


      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenBovine RPE microsomal membranes.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityReacts with Retinal Pigment Epithelium 65 (RPE65). On bovine RPE membranes the antibody recognizes a protein with a molecular weight of ~65 kDa.
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Xenopus
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.
      Gene Symbol
      • RPE65
      • mRPE65
      • rd12
      • LCA2
      • p63
      • RP20
      • sRPE65
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16518 # Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis- retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (By similarity).
      SIZE: 533 amino acids; 60948 Da
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane; Lipid-anchor (By similarity). Note=Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated (By similarity).
      TISSUE SPECIFICITY: Retinal pigment epithelium specific.
      PTM: Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all- trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A) (By similarity).
      DISEASE: SwissProt: Q16518 # Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children. & Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:180069]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant.
      SIMILARITY: SwissProt: Q16518 ## Belongs to the carotenoid oxygenase family.
      Molecular Weight~ 65 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      MAB5428 04053252664472


      Anti-Retinal Pigment Epithelium 65 Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-Retinal Pigment Epithelium 65 Antibody Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-RETINAL PIGMENT EPITHELIUM 65 (RPE65) - 3296157 3296157
      MOUSE ANTI-RETINAL PIGMENT EPITHELIUM 65 (RPE65) - 3454423 3454423
      MOUSE ANTI-RETINAL PIGMENT EPITHELIUM 65 (RPE65) - 3681256 3681256
      MOUSE ANTI-RETINAL PIGMENT EPITHELIUM 65 (RPE65) - 3880997 3880997
      MOUSE ANTI-RETINAL PIGMENT EPITHELIUM 65 (RPE65) - 3988501 3988501
      MOUSE ANTI-RETINAL PIGMENT EPITHELIUM 65 (RPE65) - 4031409 4031409
      MOUSE ANTI-RETINAL PIGMENT EPITHELIUM 65 (RPE65) - 4067703 4067703
      MOUSE ANTI-RETINAL PIGMENT EPITHELIUM 65 (RPE65) - 4097767 4097767


      Reference overviewApplicationPub Med ID
      Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelium cells.
      Schwarz, N; Carr, AJ; Lane, A; Moeller, F; Chen, LL; Aguilà, M; Nommiste, B; Muthiah, MN; Kanuga, N; Wolfrum, U; Nagel-Wolfrum, K; da Cruz, L; Coffey, PJ; Cheetham, ME; Hardcastle, AJ
      Human molecular genetics  24  972-86  2015

      Show Abstract
      25292197 25292197
      Retinoid uptake, processing, and secretion in human iPS-RPE support the visual cycle.
      Muñiz, A; Greene, WA; Plamper, ML; Choi, JH; Johnson, AJ; Tsin, AT; Wang, HC
      Investigative ophthalmology & visual science  55  198-209  2014

      Show Abstract
      24255038 24255038
      Attenuation of choroidal neovascularization by β(2)-adrenoreceptor antagonism.
      Lavine, JA; Sang, Y; Wang, S; Ip, MS; Sheibani, N
      JAMA ophthalmology  131  376-82  2013

      Show Abstract
      23303344 23303344
      Small molecule mediated proliferation of primary retinal pigment epithelial cells.
      Swoboda, JG; Elliott, J; Deshmukh, V; de Lichtervelde, L; Shen, W; Tremblay, MS; Peters, EC; Cho, CY; Lu, B; Girman, S; Wang, S; Schultz, PG
      ACS chemical biology  8  1407-11  2013

      Show Abstract
      23621521 23621521
      Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa.
      Tucker, BA; Mullins, RF; Streb, LM; Anfinson, K; Eyestone, ME; Kaalberg, E; Riker, MJ; Drack, AV; Braun, TA; Stone, EM
      eLife  2  e00824  2013

      Show Abstract
      23991284 23991284
      MEK-ERK signaling in adult newt retinal pigment epithelium cells is strengthened immediately after surgical induction of retinal regeneration.
      Aki Mizuno,Hirofumi Yasumuro,Taro Yoshikawa,Wataru Inami,Chikafumi Chiba
      Neuroscience letters  523  2012

      Show Abstract
      22743657 22743657
      c-Met modulates RPE migratory response to laser-induced retinal injury.
      Kasaoka, M; Ma, J; Lashkari, K
      PloS one  7  e40771  2012

      Show Abstract
      Immunohistochemistry22808260 22808260
      FATP1 inhibits 11-cis retinol formation via interaction with the visual cycle retinoid isomerase RPE65 and LRAT.
      Guignard TJ, Jin M, Pequignot MO, Li S, Chassigneux Y, Chekroud K, Guillou L, Richard E, Hamel CP, Brabet P
      J Biol Chem  2010

      Show Abstract
      20356843 20356843
      Musashi-1, an RNA-binding protein, is indispensable for survival of photoreceptors.
      Kanako Susaki,Jun Kaneko,Yuka Yamano,Kenta Nakamura,Wataru Inami,Taro Yoshikawa,Yoko Ozawa,Shinsuke Shibata,Osamu Matsuzaki,Hideyuki Okano,Chikafumi Chiba
      Experimental eye research  88  2009

      Show Abstract
      18662689 18662689
      Visual cycle protein RPE65 persists in new retinal cells during retinal regeneration of adult newt.
      Chikafumi Chiba, Akika Hoshino, Kenta Nakamura, Kanako Susaki, Yuka Yamano, Yuko Kaneko, Osamu Kuwata, Fumiaki Maruo, Takehiko Saito
      The Journal of comparative neurology  495  391-407  2006

      Show Abstract
      16485283 16485283

      Data Sheet