Product Information
Applications
Key Applications Paraffin Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesImmunoblotting (5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2.5-5 µg/ml, see comments)
Application CommentsThis antibody can be used as a marker for proliferation. Will stain paraffin sections without pre-treatment, but pre-treating with trypsin or a pressure cooker enhances staining. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenreplication protein A purified from U293 cells
ImmunogenHuman
EpitopeWithin the p34 subunit of replication protein A
CloneRPA34-19
HostMouse
IsotypeIgG2a
Species Reactivity
  • Hamster
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19) SDS

Title

Safety Data Sheet (SDS) 

Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19) Certificates of Analysis

TitleLot Number
NA18

References

Reference overview
Din, S., et al. 1990. Genes Dev. 4, 968.
Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
SynonymsAnti-RP-A
ApplicationImmunoblotting (5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2.5-5 µg/ml, see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~34 kDa phosphorylated subunit and the nonphosphorylated RPA complex.
BackgroundThe replication system of a cell contains several essential components among which are replication factor A (RFA, RPA, or HeLa cell SSB), PCNA, and replication factor C (RFC) as well as several other proteins. RPA, a multi-subunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein, may play a critical regulatory role in replication based on its functioning during both the initiation and elongation stages of DNA replication. The protein is highly conserved, having been identified in a range of species from yeast (Saccharomyces cerevisiae) to humans. The primary function of RPA appears to be as an auxiliary protein for both DNA polymerase α and δ. RPA undergoes a cell cycle dependent phosphorylation, which is restricted to the p34 protein. In addition to existing as part of the multi-subunit structure, p34 has also been identified as being present in a free, unphosphorylated form. Recent data shows that RPA is restricted to the nucleus and is overexpressed in transformed cells as well as in several tumor cells.
HostMouse
Immunogen speciesHuman
Immunogenreplication protein A purified from U293 cells
EpitopeWithin the p34 subunit of replication protein A
CloneRPA34-19
IsotypeIgG2a
Specieshamster, human
Positive controlHeLa or U293 cells or breast carcinoma tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody can be used as a marker for proliferation. Will stain paraffin sections without pre-treatment, but pre-treating with trypsin or a pressure cooker enhances staining. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies