MABE196 Anti-Rb2/p130 Antibody, clone EP2141Y, rabbit monoclonal

MABE196
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, FC, IH(P) Rb Diluted Culture Supernatant Monoclonal Antibody
      Description
      Catalogue NumberMABE196
      DescriptionAnti-Rb2/p130 Antibody, clone EP2141Y, rabbit monoclonal
      Alternate Names
      • retinoblastoma-like 2 (p130)
      • Retinoblastoma-related protein 2
      • 130 kDa retinoblastoma-associated protein
      • retinoblastoma-like protein 2
      Background Informationp130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of down-regulation of p130 expression in proliferating cells was investigated and results indicate that the decline of p130 expression as G0 cells reenter the cell cycle is due to a decrease in protein stability. In vitro polyubiquitination of p130 by SCF(Skp2) was specifically dependent on phosphorylation of p130 on Serine 672 (1). The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This phosphorylation is dependent upon the activity of cyclin D/cdk4. In contrast to pRB and p107, p130 is phosphorylated during G0 and the early G1 phase of the cell cycle. Also it is thought that p130, forming a complex with Rad50 through RINT-1, blocks telomerase-independent telomere lengthening in normal cells. Data indicates complementary roles for the Rb/E2F pathway in the control of telomere length (3).
      References
      Product Information
      FormatDiluted Culture Supernatant
      Control
      • HeLa and A431 cell lysates
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      Application NotesFlow Cytometry Analysis: 1:20 dilution from a representative lot was used in FC.
      Immunohistochemistry Analysis: 1:50-100 dilution from a representative lot detected Rb2/p130 in human breast carcinoma tissue.
      Biological Information
      ImmunogenSynthetic peptide corresponding to human Rb2/p130 near the C-terminus.
      EpitopeNear C-terminus
      CloneEP2141Y
      HostRabbit
      SpecificityThis antibody recognizes Rb2/p130 near the C-terminus.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Rb2
      • p130
      • RBR-2
      • FLJ26459
      • PRB2
      • P130
      • pRb2
      • RB2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
      SUBUNIT STRUCTURE: Interacts with AATF. Interacts with SUV420H1, SUV420H2 and USP4 By similarity. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with RINT1.
      SUBCELLULAR LOCATION: Nucleus.
      DEVELOPMENTAL STAGE: G0-restricted expression.
      PTM: During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
      SEQUENCE SIMILARITES: Belongs to the retinoblastoma protein (RB) family.
      Molecular Weight~130 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.
      Western Blot Analysis: 1:500 dilution of this antibody detected Rb2/p130 in Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below
      -20°C may cause glycerol containing Solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies