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553571 Anti-Ras (31-43) Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
B, H, M, R Rb Polyclonal Antibody

Pricing & Availability

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553571-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewRecognizes all forms of wild type Ras, including the mutant G12V Ras protein.
      Catalogue Number553571
      Brand Family Calbiochem®
      SynonymsAnti-Ras GDP/GTP Binding Protein
      Application Data
      Detection of rat Ras by immunoblotting. Sample: Whole tissue extract from rat brain. Primary antibody: Anti-Ras (31-43) Rabbit pAb (Cat. No. 553571) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesHardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418, Academic Press, San Diego, CA.
      Sawada, M., et al. 1989. Nucleic Acid Res. 17, 8867.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Positive controlRat brain tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1 µg/ml)
      Application CommentsBy immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Ras (31-43) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Ras (31-43) Rabbit pAb Certificates of Analysis

      TitleLot Number
      553571

      References

      Reference overview
      Hardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418, Academic Press, San Diego, CA.
      Sawada, M., et al. 1989. Nucleic Acid Res. 17, 8867.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-January-2009 RFH
      SynonymsAnti-Ras GDP/GTP Binding Protein
      ApplicationImmunoblotting (1 µg/ml)
      Application Data
      Detection of rat Ras by immunoblotting. Sample: Whole tissue extract from rat brain. Primary antibody: Anti-Ras (31-43) Rabbit pAb (Cat. No. 553571) (1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity rabbit polyclonal antibody. Recognizes all forms of wild type Ras and the mutant G12V Ras protein.
      BackgroundRas proteins bind GDP and GTP with high affinity. This region of Ras is important in the binding of Ras to its effector, Raf1. This sequence (residues 31-43) is nearly 100% conserved between the different Ras encoded proteins (H-, K-, and N-Ras) and through phylogeny from slime mold to man.
      HostRabbit
      Immunogena synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH
      IsotypeIgG
      Speciesbovine, human, mouse, rat
      Positive controlRat brain tissue
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsBy immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418, Academic Press, San Diego, CA.
      Sawada, M., et al. 1989. Nucleic Acid Res. 17, 8867.