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04-037 Anti-RalB Antibody, clone 25

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04-037
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-037
      Trade Name
      • Upstate
      DescriptionAnti-RalB Antibody, clone 25
      Alternate Names
      • RAS-like protein B
      • v-ral simian leukemia viral oncogene homolog B
      • GTP binding protein
      • v-ral simian leukemia viral oncogene homolog B
      Background InformationRalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarizatied exocystossis in epithelaial cells (Cascone, 2008). RalB is required for metastatic formation and cell survival by driving exocyst assembly during polarized cell migration (Cascone, 2008). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect RalB using this Anti-RalB Antibody, clone 25 validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenSynthetic peptide of the C-terminus of RalB
      EpitopeC-terminus
      CloneClone 25
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody is specific for RalB and does not detect RalA.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, and Rat. Other species have not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors.
      Gene Symbol
      • RALB
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummarySUBUNIT STRUCTURE: Interacts with EXOC8. Interacts with RALBP1 via its effector domain. Ref.6 Ref.7
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic sidePotential.
      SEQUENCE SIMILARITIES: Belongs to the small GTPase superfamily. Ras family.
      Molecular Weight24 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on HEK293 or NIH/3T3 cell lysates.
      Western Blot Analysis:
      1:1,000 dilution of this antibody was used to detect RalB in HEK293, NIH/3T3, and rat liver cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-RalB Antibody, clone 25 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-RalB Antibody, clone 25 Certificates of Analysis

      TitleLot Number
      Anti-RalB (clone 25) (Purified Mouse Monoclonal IgG) - DAM1483560 DAM1483560
      Anti-RalB, clone 25 - 2137084 2137084
      Anti-RalB, clone 25 - 1947718 1947718
      Anti-RalB, clone 25 - 2018119 2018119
      Anti-RalB, clone 25 - 2549986 2549986
      Anti-RalB, clone 25 - 3589743 3589743
      Anti-RalB, clone 25 - DAM1745372 DAM1745372
      Anti-RalB, clone 25 - DAM1776478 DAM1776478
      Anti-RalB, clone 25 - JBC1900513 JBC1900513
      Anti-RalB, clone 25 - NG1605292 NG1605292

      References

      Reference overviewPub Med ID
      Discovery and characterization of small molecules that target the GTPase Ral.
      Yan, C; Liu, D; Li, L; Wempe, MF; Guin, S; Khanna, M; Meier, J; Hoffman, B; Owens, C; Wysoczynski, CL; Nitz, MD; Knabe, WE; Ahmed, M; Brautigan, DL; Paschal, BM; Schwartz, MA; Jones, DN; Ross, D; Meroueh, SO; Theodorescu, D
      Nature  515  443-7  2014

      Show Abstract
      25219851 25219851
      Genetic Deletion of RALA and RALB Small GTPases Reveals Redundant Functions in Development and Tumorigenesis.
      Pascal Peschard,Afshan McCarthy,Val Leblanc-Dominguez,Maggie Yeo,Sabrina Guichard,Gordon Stamp,Christopher J Marshall
      Current biology : CB  22  2012

      Show Abstract
      23063435 23063435
      RalA and RalB proteins are ubiquitinated GTPases, and ubiquitinated RalA increases lipid raft exposure at the plasma membrane.
      Neyraud, V; Aushev, VN; Hatzoglou, A; Meunier, B; Cascone, I; Camonis, J
      The Journal of biological chemistry  287  29397-405  2012

      Show Abstract
      22700969 22700969
      RalB mobilizes the exocyst to drive cell migration.
      Rossé, Carine, et al.
      Mol. Cell. Biol., 26: 727-34 (2006)  2006

      Show Abstract
      16382162 16382162
      RalA but not RalB enhances polarized delivery of membrane proteins to the basolateral surface of epithelial cells.
      Shipitsin, Michail and Feig, Larry A
      Mol. Cell. Biol., 24: 5746-56 (2004)  2004

      Show Abstract
      15199131 15199131
      RAL GTPases are linchpin modulators of human tumour-cell proliferation and survival.
      Chien, Yuchen and White, Michael A
      EMBO Rep., 4: 800-6 (2003)  2003

      Show Abstract
      12856001 12856001
      Calmodulin binds RalA and RalB and is required for the thrombin-induced activation of Ral in human platelets
      Clough, Richard R, et al
      J Biol Chem, 277:28972-80 (2002)  2002

      12034722 12034722