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MABS2055-100UG Anti-RNase L Antibody, clone 2E9

MABS2055-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2055-100UG
      DescriptionAnti-RNase L Antibody, clone 2E9
      Alternate Names
      • 2-5A-dependent ribonuclease
      • 2-5A-dependent RNase
      • Ribonuclease 4
      • Ribonuclease L
      Background Information2-5A-dependent ribonuclease (UniProt: Q05823; also known as 2-5A-dependent RNase, Ribonuclease 4, Ribonuclease L, RNase L) is encoded by the RNASEL (also known as RNS4) gene (Gene ID: 6041) in human. RNAse L is a virally induced endoribonuclease that functions in the interferon antiviral response. It is highly expressed in spleen and thymus followed by prostate, testis, uterus, small intestine, colon, and peripheral blood leukocytes. It is present in cells in a latent form and requires short 5 - phosphorylated, 2-5A for its activity. Activation of RNAse L results in inhibition of viral proliferation. It mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. Activation of RNAse L has also been shown to degrade RNA in a manganese or magnesium dependent process. It has a relatively broad specificity for cleaving single-stranded RNA but prefers UU and UA dinucleotides. RNase-mediated apoptosis results from a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. RNAse L contains nine ankyrin repeats, which constitute the N-terminus 2-5A binding domain. It also has a C6-type zinc finger domain (aa 395-444). Its ribonuclease domain is located in the C-terminus and a single active nuclease domain in a dimer is shown to be sufficient for its ribonuclease activity. Mutations in RNASEL gene are reported in cancers of the prostate and other tissues. (Ref.: Wang, L., et al. (1995). Clin. Can. Res. 1 (11); 1421-1428; Dong, B., and Silverman, RH (1995). J. Biol. Chem. 270(8); 4133-4137; Chakrabarti, A et al. (2011). J. Interferon Cytokine Res.31(1); 49-57).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-RNase L, clone 2E9, Cat. No. MABS2055, is a mouse monoclonal antibody that detects 2-5A-dependent ribonuclease(RNase L) and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected RNase L in human stomach tissue sections.

      Western Blotting Analysis: A representative lot detected RNase L in Western Blotting applications (Dong, B., et. al. (1995). J Biol Chem. 270(8):4133-7).

      ELISA Analysis: A representative lot detected RNase L in ELISA applications (Dong, B., et. al. (1995). J Biol Chem. 270(8):4133-7).

      Immunohistochemistry Analysis: A representative lot detected RNase L in Immunohistochemistry applications (Wang, L., et. al. (1995). Clin Cancer Res. 1(11):1421-8).
      Biological Information
      ImmunogenFull length recombinant human 2-5A-dependent ribonuclease (RNase L).
      Clone2E9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2E9 is a mouse monoclonal antibody that detects Ribonuclease L in human cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNASEL
      • RNS4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~83.53 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human prostate tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected RNase L in human prostate tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-RNase L Antibody, clone 2E9 Certificates of Analysis

      TitleLot Number
      Anti-RNase L, clone 2E9 - Q3224758Q3224758

      References

      Reference overviewPub Med ID
      2-5A-dependent RNase molecules dimerize during activation by 2-5A.
      Dong, B; Silverman, RH
      J Biol Chem  271  4133-8  1996

      Show Abstract
      7876164 7876164
      Elevated levels of 2',5'-linked oligoadenylate-dependent ribonuclease L occur as an early event in colorectal tumorigenesis.
      Wang, L; Zhou, A; Vasavada, S; Dong, B; Nie, H; Church, JM; Williams, BR; Banerjee, S; Silverman, RH
      Clin Cancer Res  2  1421-9  1996

      Show Abstract
      9815940 9815940