DR1015 Anti-RENT1 Goat pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H Gt Polyclonal Antibody

Pricing & Availability

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DR1015-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~140 kDa RENT1 protein in HeLa cells.
      Catalogue NumberDR1015
      Brand Family Calbiochem®
      SynonymsAnti-HUPF1, Anti-NORF1
      Application Data
      Detection of human RENT1 by immunoblotting. Samples: Whole cell lysate from untreated (-) and irradiated (+) lymphophoid cell lines (LCL1 and LCL2; EBV-immortalized B-cell lines from a normal individuals). Primary antibody: Anti-RENT1 Goat pAb (Cat. No. DR1015) (1:1000). Detection: chemiluminescence.
      References
      ReferencesMendell, J.T., et al. 2002. Science 298, 419.
      Medghalachi, S.M., et al. 2001. Hum. Mol. Genet. 10, 99.
      Product Information
      FormLiquid
      FormulationIn Tris/citrate phosphate buffer, pH 7.0-8.0.
      Positive controlHeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000-1:2500)
      Application CommentsMay detect an additional band at ~80 kDa. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human RENT1
      ImmunogenHuman
      HostGoat
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-RENT1 Goat pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-RENT1 Goat pAb Certificates of Analysis

      TitleLot Number
      DR1015

      References

      Reference overview
      Mendell, J.T., et al. 2002. Science 298, 419.
      Medghalachi, S.M., et al. 2001. Hum. Mol. Genet. 10, 99.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      SynonymsAnti-HUPF1, Anti-NORF1
      ApplicationImmunoblotting (1:1000-1:2500)
      Application Data
      Detection of human RENT1 by immunoblotting. Samples: Whole cell lysate from untreated (-) and irradiated (+) lymphophoid cell lines (LCL1 and LCL2; EBV-immortalized B-cell lines from a normal individuals). Primary antibody: Anti-RENT1 Goat pAb (Cat. No. DR1015) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified goat polyclonal antibody. Recognizes the ~140 kDa RENT1 protein.
      BackgroundEukaryotes have the ability to recognize and degrade transcripts that harbor premature signals for translational termination. Nonsense and frameshift mutations that result in these premature termination codons play a role in many genetic diseases and some cancers. Nonsense-mediated nRNA decay (NMD) is mediated by several factors including rent1/hUpf1, rent2/hUpf2, and hUpf3. When assembled, these proteins form the surveillence complex. Disruption of the mouse Rent1 gene has been reported to completely disrupt NMD and result in embryos that re-absorb shortly after implantation.
      HostGoat
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human RENT1
      IsotypeIgG
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationIn Tris/citrate phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsMay detect an additional band at ~80 kDa. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Toxicity Highly Toxic
      ReferencesMendell, J.T., et al. 2002. Science 298, 419.
      Medghalachi, S.M., et al. 2001. Hum. Mol. Genet. 10, 99.