09-100 Anti-RBL2 Antibody

09-100
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number09-100
      DescriptionAnti-RBL2 Antibody
      Alternate Names
      • 130 kDa retinoblastoma-associated protein
      • retinoblastoma-like 2 (p130)
      Background InformationRBL2, retinoblastoma-like protein 2 also referred to as a pocket protein, belongs to the RB family of tumor suppressors, as does another pocket protein p107. These pocket proteins are involved in cell proliferation, differentiation, and transformation. They also share a significant amount of sequence homology. RBL2 is an important regulator of entry in cell division and may act as a transcription repressor by recruiting chromatin-modifying enzymes to promoters.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Raji cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-RBL2 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect RBL2 also known as 130 kDa retinoblastoma-associated protein.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear protein corresponding to human RBL2 at and around the C-terminus.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes RBL2 at and around the C-terminus.
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human. Predicted to react with rat, horse, chimpanzee, ape, and dog based on sequence homology. Mouse (95%).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RBL2
      • P130
      • PRB2
      • RB2
      • RBR-2
      • Rb2
      • p130
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

      SIZE: 1139 amino acids; 128367 Da

      SUBUNIT: Interacts with AATF. Interacts with SUV420H1 and SUV420H2 (By similarity). Interacts with RINT1.

      SUBCELLULAR LOCATION: Nucleus.

      PTM: During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.

      SIMILARITY: Belongs to the retinoblastoma protein (RB) family.

      MISCELLANEOUS: G0-restricted expression.
      Molecular Weight130 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in raji cell lysate.
      Western Blot Analysis: 1 µg/mL of this antibody detected RBL2 on 10 µg of raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-RBL2 Antibody Certificates of Analysis

      TitleLot Number
      Anti-RBR2 (RBL2) - NRG1644433NRG1644433

      References

      Reference overviewPub Med ID
      Detection of toxin translocation into the host cytosol by surface plasmon resonance.
      Michael Taylor,Tuhina Banerjee,Neyda Vanbennekom,Ken Teter
      Journal of visualized experiments : JoVE  2012

      Show Abstract
      22231143 22231143

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies