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MAB5328 Anti-RAGE Antibody, clone DD/A11 or A11

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MAB5328
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, MELISA, ICC, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5328
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-RAGE Antibody, clone DD/A11 or A11
      Alternate Names
      • Receptor for Advanced Glycosylation End Products.
      Background InformationRAGE (Receptor for Advanced Glycosylation End Products) is a 35 kDa cell surface receptor that binds molecules modified by advanced glycation end products (AGEs), an irreversible non-enzymatic reaction occuring from the interaction of proteins and lipids with glucose. The increased expression of RAGE is associated with many disorders, such as diabetic vasculopathy, neuropathy, retinopathy and neuropathy, Alzheimer’s disease and immune/inflammatory reactions of the vessel walls. AGE/RAGE accumulates at sites of vascular disease in diabetes, but blockade of RAGE suppresses development of atherosclerosis and vascular disorders. RAGE binds to amyloid beta, which is overproduced in neurons and vessels in Alzheimer's disease, and this interaction results in oxidative stress leading to neuronal degeneration.
      References
      Product Information
      FormatPurified
      Control
      • Lung tissue, mouse brain lysate.
      PresentationPurified mouse monoclonal IgG2a in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RAGE Antibody, clone DD/A11 or A11 is an antibody against RAGE for use in ELISA, IC, IH & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was shown to be reactive on paraffin embedded tissue sections.

      Immunocytochemistry:
      A previous lot was shown to work on cells fixed with ice cold acetone using a Cy3 conjugated secondary antibody.

      ELISA:
      A previous lot was shown to work on direct ELISA.

      Immunoblotting:
      Recognizes a band of ~48kDa in bovine lung extract.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant truncated extracellular part of RAGE produced in E. coli.
      EpitopeExtracellular
      CloneDD/A11 or mAbA11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRAGE (Receptor for Advanced Glycosylated End Products). The antibody recognizes both natural and recombinant RAGE.
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      Species Reactivity NoteMouse. Expected to react with rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAGE
      • RAGE-1
      • MOK
      • RAGE1
      • EC 2.7.11.22
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15109 # Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Receptor for amyloid beta peptide.
      SIZE: 404 amino acids; 42803 Da
      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
      TISSUE SPECIFICITY: Endothelial cells.
      PTM: Autophosphorylated (By similarity).
      SIMILARITY: SwissProt: Q15109 # Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
      Molecular Weight48 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

      Western Blot:
      1:500 dilution of this lot detected RAGE on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-RAGE Antibody, clone DD/A11 or A11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-RAGE Antibody, clone DD/A11 or A11 Certificates of Analysis

      TitleLot Number
      Anti-RAGE, clone DD/A11 or mAbA11 - 2118037 2118037
      Anti-RAGE, clone DD/A11 or mAbA11 - 2428726 2428726
      Anti-RAGE, clone DD/A11 or mAbA11 - 2443160 2443160
      Anti-RAGE, clone DD/A11 or mAbA11 - 1983683 1983683
      Anti-RAGE, clone DD/A11 or mAbA11 - 2041579 2041579
      Anti-RAGE, clone DD/A11 or mAbA11 - 2300465 2300465
      Anti-RAGE, clone DD/A11 or mAbA11 - 2942483 2942483
      Anti-RAGE, clone DD/A11 or mAbA11 - 3225898 3225898
      Anti-RAGE, clone DD/A11 or mAbA11 - 3283673 3283673
      Anti-RAGE, clone DD/A11 or mAbA11 - 3448963 3448963

      References

      Reference overviewApplicationSpeciesPub Med ID
      Receptor for Advanced Glycation End-Products Signaling Interferes with the Vascular Smooth Muscle Cell Contractile Phenotype and Function.
      Simard, E; Söllradl, T; Maltais, JS; Boucher, J; D'Orléans-Juste, P; Grandbois, M
      PloS one  10  e0128881  2015

      Show Abstract
      26248341 26248341
      Statins stimulate the production of a soluble form of the receptor for advanced glycation end products.
      Quade-Lyssy, P; Kanarek, AM; Baiersdörfer, M; Postina, R; Kojro, E
      Journal of lipid research  54  3052-61  2013

      Show Abstract
      23966666 23966666
      AGEs-RAGE mediated up-regulation of connexin43 in activated human microglial CHME-5 cells.
      Shamim B Shaikh,Benedict Uy,Amali Perera,Louise F B Nicholson
      Neurochemistry international  60  2012

      Show Abstract
      22417726 22417726
      Induction of RAGE shedding by activation of G protein-coupled receptors.
      Metz, VV; Kojro, E; Rat, D; Postina, R
      PloS one  7  e41823  2012

      Show Abstract
      22860017 22860017
      Fisetin lowers methylglyoxal dependent protein glycation and limits the complications of diabetes.
      Maher, P; Dargusch, R; Ehren, JL; Okada, S; Sharma, K; Schubert, D
      PloS one  6  e21226  2011

      Show Abstract
      Western BlottingMouse21738623 21738623
      Gradual increase of high mobility group protein b1 in the lungs after the onset of acute exacerbation of idiopathic pulmonary fibrosis.
      Ebina, M; Taniguchi, H; Miyasho, T; Yamada, S; Shibata, N; Ohta, H; Hisata, S; Ohkouchi, S; Tamada, T; Nishimura, H; Ishizaka, A; Maruyama, I; Okada, Y; Takashi, K; Nukiwa, T
      Pulmonary medicine  2011  916486  2011

      Show Abstract Full Text Article
      21637372 21637372
      Alternatively spliced RAGEv1 inhibits tumorigenesis through suppression of JNK signaling.
      Kalea, Anastasia Z, et al.
      Cancer Res., 70: 5628-38 (2010)  2010

      Show Abstract
      20570900 20570900
      Neural-activity-dependent release of S100B from astrocytes enhances kainate-induced gamma oscillations in vivo.
      Sakatani, S; Seto-Ohshima, A; Shinohara, Y; Yamamoto, Y; Yamamoto, H; Itohara, S; Hirase, H
      The Journal of neuroscience : the official journal of the Society for Neuroscience  28  10928-36  2008

      Show Abstract
      18945900 18945900
      Receptor for advanced glycation end products is subjected to protein ectodomain shedding by metalloproteinases.
      Zhang, L; Bukulin, M; Kojro, E; Roth, A; Metz, VV; Fahrenholz, F; Nawroth, PP; Bierhaus, A; Postina, R
      The Journal of biological chemistry  283  35507-16  2008

      Show Abstract
      18952609 18952609
      A soluble form of the receptor for advanced glycation endproducts (RAGE) is produced by proteolytic cleavage of the membrane-bound form by the sheddase a disintegrin and metalloprotease 10 (ADAM10).
      Raucci, A; Cugusi, S; Antonelli, A; Barabino, SM; Monti, L; Bierhaus, A; Reiss, K; Saftig, P; Bianchi, ME
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  22  3716-27  2008

      Show Abstract
      18603587 18603587

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)