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AB9714-I Anti-RAGE

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AB9714-I
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9714-I
      ReplacesAB9714
      DescriptionAnti-RAGE
      Alternate Names
      • Advanced glycosylation end product-specific receptor
      • Receptor for advanced glycosylation end products
      • RAGE
      Background InformationAdvanced glycosylation end product-specific receptor (UniProt: Q15109; also known as Receptor for advanced glycosylation end products, RAGE) is encoded by the AGER (also known as RAGE) gene (Gene ID: 177) in human. RAGE is a single-pass type I membrane protein that contains two Ig-like C2-type domains, one Ig-like V-type domain, a 21 amino acid transmembrane domain and a 41 amino acids cytoplasmic domain. RAGE mediates interactions of advanced glycosylation end products (AGE), which are non-enzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. RAGE acts as a mediator of both acute and chronic vascular inflammation in conditions, such as atherosclerosis and in complication of diabetes. Blockage of RAGE is reported to suppress development of atherosclerosis and vascular disorders. RAGE is expressed by endothelium, mononuclear phagocytes, neurons and smooth muscle cells. Higher expression of RAGE is observed during development, especially in the central nervous system, and its levels decline during maturity. Besides binding to advanced glycosylation end product (AGE), RAGE also binds amyloid beta peptide, S100family proteins, and leukocyte integrins. Excessive amounts of amyloid peptides (Ab) in brain leads to the hyperstimulation of RAGE. The RAGE-Ab interaction is thought to result in oxidative stress leading to neuronal degeneration.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RAGE, Cat. No. AB9714-I, is a highly specific rabbit polyclonal antibody that targets Advanced glycosylation end product-specific receptor and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected RAGE in human lung tissue.
      Biological Information
      ImmunogenKLH-cojugated linear peptide corresponding to 20 amino acids from the N-terminal region of human RAGE.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects Receptor for Advanced Glycosylation End Products (RAGE). This antibody targets an epitope corresponding to 21 amino acids in the N-terminal region of human RAGE. Human, mouse, and rat RAGE display 95% homology in this epitope region.
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteRat, Mouse. Predicted to react with Human based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AGER
      • RAGE
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~48 kDa observed; 42.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse lung tissue lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected RAGE in 10 µg of mouse lung tissue lysate.


      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications