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AB10181 Anti-Pumilio-2 (PUM2) Antibody

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AB10181
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Chp, Rhesus Macaque, CaICC, IHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB10181
      DescriptionAnti-Pumilio-2 (PUM2) Antibody
      Alternate Names
      • pumilio homolog 2 (Drosophila)
      • pumilio homolog 2
      • pumilio (Drosphila) homolog 2
      Background InformationPUM2 is a member of the Puf family of RNA-binding proteins, which are believed to play a role in the regulation of posttranscriptional gene expression. Members of the Puf family of RNA binding proteins function as repressors of translation when bound to the 3-untranslated region of RNA transcripts.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse embryonic stem cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Pumilio-2 (PUM2) using this Anti-Pumilio-2 (PUM2) Antibody validated for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:400 dilution from a previous lot detected Pumilio-2 in human testis and teratoma tissue.

      Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Pumilio-2 in human and mouse embryonic stem cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Pumilio-2.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Pumilio-2 near the N-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Chimpanzee
      • Rhesus Macaque
      • Canine
      Species Reactivity NoteDemonstrated to react with human and mouse.
      Predicted to react with chimpanzee, rhesus monkey, and canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PUM2
      • PUML2
      • PUMH2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.

      SUBUNIT STRUCTURE: Homodimer; homodimerizes in vitro. Interacts with DAZ, DAZL and NANOS1 via its pumilio repeats. Binds to a RNA consensus sequence, that is related to the Nanos Response Element (NRE), a 16 bp sequence found in the 3'-UTR of the Drosophila hb mRNA. Also binds to the NRE.

      SUBCELLULAR LOCATION: Cytoplasm Probable.

      TISSUE SPECIFICITY: Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.

      INDUCTION: Down-regulated in keratinocytes upon UV B radiation.

      DOMAIN: The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half donut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs By similarity.

      SEQUENCE SIMILARITIES: Contains 1 PUM-HD domain.

      Contains 8 pumilio repeats.
      Molecular Weight~120 kDa observed.
      Non specific band may be observed at 48 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on mouse embryonic stem cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Pumilio-2 (PUM2) in 10 µg of mouse embryonic stem cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies