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MAB1574 Anti-Polyglutamine-Expansion Diseases Marker Antibody, clone 5TF1-1C2

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MAB1574
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, ICC, IH(P), IP, WBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB1574
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Polyglutamine-Expansion Diseases Marker Antibody, clone 5TF1-1C2
      Alternate Names
      • Poly-Glu
      • PolyQ
      Background InformationHuntington’s disease (HD) belongs to a family of polyglutamine diseases, which includes dentatorubralpallidoluysian atrophy (DRPLA), spinobulbar muscular atrophy (SBMA) and spinocerebellar ataxia (SCA) types 1–3, 6, 7 and 17. In these diseases, the non-pathogenic alleles contain fewer than approximately 35 consecutive glutamine repeats and encode a normal polyglutamine domain. In contrast, the pathogenic alleles usually contain 39 or more consecutive glutamine repeats. Higher repeat numbers lead to lower ages of onset. Patients with 40-60 glutamine repeats normally develop disease as adults, whereas patients with more than 60 repeats develop a juvenile onset disease. Each polyglutamine expansion disorder displays characteristic pathology, with neuronal loss evident in specific regions of the brain. HD results from expansions of a glutamine tract in a large cystolic protein known as huntingtin.
      References
      Product Information
      FormatAscites
      HS Code3002 15 90
      Control
      • Huntigton's Disease brain
      PresentationAscites fluid containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Polyglutamine-Expansion Diseases Marker Antibody, clone 5TF1-1C2 is an antibody against Polyglutamine-Expansion Diseases Marker for use in ELISA, IC, IH(P), IP & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesELISA: 1:1,000-1:20,000

      Western Blot: 1:1,000-1:20,000

      Immunohistochemistry on frozen and paraffin sections (human tissue): 1:1,000-1:20,000

      Immunocytochemistry on transfected cells: 1:1,000-1:20,000 Immunoprecipitation: 1:1,000-1:20,000

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenN-terminal part of the human TATA Box Binding Protein (TBP).
      Clone5TF1-1C2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe epitope of MAB1574 was found to be a homopolymeric glutamine stretch. The original immunogen was the general transcription factor TATA Box-binding protein (TBP) which contains a 38-glns stretch (Lescure et al). Other polyglutamine-containing proteins are recognized by the MAB1574, notably those involved in several human neurodegenerative diseases caused by a CAG repeat expansion, like Huntington's disease and spinocerebellar ataxia type 2, 3 and 7 (Trottier et al., 1995). Importantly, for proteins involved in these neurodegenerative disorders, MAB1574 showed remarkable property of detecting much better the pathological proteins that contain a polyglutamine expansion (37 glns) than the wild type proteins (Trottier et al., 1995). MAB1574 has been used to identify new neurodegenerative diseases caused by polyglutamine expansion and to help for cloning of the corresponding affected genes (Trottier 1995-1998; Imbert 1996; Stevanin 1996). MAB1574 is also able to detect intracellular inclusions, which is a hallmark of such diseases (Paulson, 1997).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Purification MethodUnpurified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB1574 04053252315916

      Documentation

      Anti-Polyglutamine-Expansion Diseases Marker Antibody, clone 5TF1-1C2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Polyglutamine-Expansion Diseases Marker Antibody, clone 5TF1-1C2 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-POLYGLUTAMINE 2464513
      MOUSE ANTI-POLYGLUTAMINE - 3317798 3317798
      MOUSE ANTI-POLYGLUTAMINE - 3447919 3447919
      MOUSE ANTI-POLYGLUTAMINE - 4101320 4101320
      MOUSE ANTI-POLYGLUTAMINE - 4158916 4158916
      MOUSE ANTI-POLYGLUTAMINE Polyglutamine-Expansion Diseases Marker - 2519210 2519210
      MOUSE ANTI-POLYGLUTAMINE Polyglutamine-Expansion Diseases Marker - 3034378 3034378
      MOUSE ANTI-POLYGLUTAMINE Polyglutamine-Expansion Diseases Marker - 3171745 3171745
      MOUSE ANTI-POLYGLUTAMINE Polyglutamine-Expansion Diseases Marker - 3189192 3189192
      MOUSE ANTI-POLYGLUTAMINE Polyglutamine-Expansion Diseases Marker - 3202405 3202405

      References

      Reference overviewApplicationPub Med ID
      Nuclear retention of full-length HTT RNA is mediated by splicing factors MBNL1 and U2AF65.
      Sun, X; Li, PP; Zhu, S; Cohen, R; Marque, LO; Ross, CA; Pulst, SM; Chan, HY; Margolis, RL; Rudnicki, DD
      Scientific reports  5  12521  2015

      Show Abstract
      Western Blotting26218986 26218986
      Impaired GAPDH-induced mitophagy contributes to the pathology of Huntington's disease.
      Hwang, S; Disatnik, MH; Mochly-Rosen, D
      EMBO molecular medicine  7  1307-26  2015

      Show Abstract
      26268247 26268247
      Inactivation of PNKP by mutant ATXN3 triggers apoptosis by activating the DNA damage-response pathway in SCA3.
      Gao, R; Liu, Y; Silva-Fernandes, A; Fang, X; Paulucci-Holthauzen, A; Chatterjee, A; Zhang, HL; Matsuura, T; Choudhary, S; Ashizawa, T; Koeppen, AH; Maciel, P; Hazra, TK; Sarkar, PS
      PLoS genetics  11  e1004834  2015

      Show Abstract
      25590633 25590633
      Proteotoxic stress induces phosphorylation of p62/SQSTM1 by ULK1 to regulate selective autophagic clearance of protein aggregates.
      Lim, J; Lachenmayer, ML; Wu, S; Liu, W; Kundu, M; Wang, R; Komatsu, M; Oh, YJ; Zhao, Y; Yue, Z
      PLoS genetics  11  e1004987  2015

      Show Abstract
      25723488 25723488
      Detection of ubiquitinated huntingtin species in intracellular aggregates.
      Juenemann, K; Wiemhoefer, A; Reits, EA
      Frontiers in molecular neuroscience  8  1  2015

      Show Abstract
      25674046 25674046
      Effects on murine behavior and lifespan of selectively decreasing expression of mutant huntingtin allele by supt4h knockdown.
      Cheng, HM; Chern, Y; Chen, IH; Liu, CR; Li, SH; Chun, SJ; Rigo, F; Bennett, CF; Deng, N; Feng, Y; Lin, CS; Yan, YT; Cohen, SN; Cheng, TH
      PLoS genetics  11  e1005043  2015

      Show Abstract
      25760041 25760041
      Ataxin-2 regulates RGS8 translation in a new BAC-SCA2 transgenic mouse model.
      Dansithong, W; Paul, S; Figueroa, KP; Rinehart, MD; Wiest, S; Pflieger, LT; Scoles, DR; Pulst, SM
      PLoS genetics  11  e1005182  2015

      Show Abstract
      25902068 25902068
      Amyloid domains in the cell nucleus controlled by nucleoskeletal protein lamin B1 reveal a new pathway of mercury neurotoxicity.
      Arnhold, F; Gührs, KH; von Mikecz, A
      PeerJ  3  e754  2015

      Show Abstract
      25699204 25699204
      HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice.
      Ito, H; Fujita, K; Tagawa, K; Chen, X; Homma, H; Sasabe, T; Shimizu, J; Shimizu, S; Tamura, T; Muramatsu, S; Okazawa, H
      EMBO molecular medicine  7  78-101  2015

      Show Abstract
      25510912 25510912
      Inhibition of mitochondrial protein import by mutant huntingtin.
      Yano, H; Baranov, SV; Baranova, OV; Kim, J; Pan, Y; Yablonska, S; Carlisle, DL; Ferrante, RJ; Kim, AH; Friedlander, RM
      Nature neuroscience  17  822-31  2014

      Show Abstract
      24836077 24836077