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AB9088 Anti-Polycystin-2 Antibody

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AB9088
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ICC, IHCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB9088
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Polycystin-2 Antibody
      References
      Product Information
      FormatSerum
      Control
      • POSITIVE CONTROL: adult mouse kidney
      PresentationRabbit serum. Liquid.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Polycystin-2 Antibody is an antibody against Polycystin-2 for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern blot: 1:500 using ECL. The antibody reacts with the 105 kDa Polycystin-2 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

      Immunocytochemistry: 1:1,000

      Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from the C-terminal of mouse Polycystin-2.
      HostRabbit
      SpecificityPolycystin-2.
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. The encoded protein may function in renal tubular development, morphology, and function, and may modulate intracellular calcium homoeostasis and other signal transduction pathways. This protein interacts with polycystin 1 to produce cation-permeable currents. Mutations in this gene have been associated with autosomal dominant polycystic kidney disease.
      Gene Symbol
      • PKD2
      • PKD4
      • Polycystwin
      • Polycystin-2
      • PC2
      • polycystwin
      • APKD2
      • R48321
      • MGC138468
      • MGC138466
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13563 # Functions as a calcium permeable cation channel. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis.
      SIZE: 968 amino acids; 109691 Da
      SUBUNIT: Interacts with PKD1. PKD1 requires the presence of PKD2 for stable expression. Interacts with CD2AP.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
      TISSUE SPECIFICITY: Strongly expressed in ovary, fetal and adult kidney, testis, and small intestine. Not detected in peripheral leukocytes.
      DOMAIN:SwissProt: Q13563 The C-terminal is implicated in the interaction with PKD1.
      DISEASE: SwissProt: Q13563 # Defects in PKD2 are the cause of polycystic kidney disease autosomal dominant type 2 (ADPKD2) [MIM:173900]. ADPKD2 represents approximately 15% of the cases of ADPKD, a common genetic disease affecting about 1:400 to 1:1000 individuals. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. ADPKD2 is clinically milder than ADPKD1 but it has a deleterious impact on overall life expectancy.
      SIMILARITY: Belongs to the polycystin family. & Contains 1 EF-hand domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Polycystin-2 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Polycystin-2 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-POLYCYSTIN-2 POLYCLONAL ANTIBODY - 2360926 2360926
      RABBIT ANTI-POLYCYSTIN-2 - 3063875 3063875
      RABBIT ANTI-POLYCYSTIN-2 - 3686340 3686340
      RABBIT ANTI-POLYCYSTIN-2 -2615048 2615048
      RABBIT ANTI-POLYCYSTIN-2 POLYCLONAL ANTIBODY 2965863
      RABBIT ANTI-POLYCYSTIN-2 POLYCLONAL ANTIBODY 2865962
      RABBIT ANTI-POLYCYSTIN-2 POLYCLONAL ANTIBODY 2970730
      RABBIT ANTI-POLYCYSTIN-2 POLYCLONAL ANTIBODY 3013585
      RABBIT ANTI-POLYCYSTIN-2 POLYCLONAL ANTIBODY 2886461
      RABBIT ANTI-POLYCYSTIN-2 POLYCLONAL ANTIBODY 3127011

      References

      Reference overviewPub Med ID
      Distribution and function of polycystin-2 in mouse retinal ganglion cells.
      S Kaja,O A Mafe,R A Parikh,P Kandula,C A Reddy,E V Gregg,H Xin,P Mitchell,M A Grillo,P Koulen
      Neuroscience  202  2012

      Show Abstract
      22155264 22155264
      The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors.
      Mühlhans, J; Brandstätter, JH; Giessl, A
      PloS one  6  e26496  2011

      Show Abstract
      22031837 22031837
      Polycystin-2 expression and function in adult mouse lacrimal acinar cells.
      Kaja, S; Hilgenberg, JD; Rybalchenko, V; Medina-Ortiz, WE; Gregg, EV; Koulen, P
      Investigative ophthalmology & visual science  52  5605-11  2011

      Show Abstract
      21508103 21508103
      The RNA-binding protein bicaudal C regulates polycystin 2 in the kidney by antagonizing miR-17 activity.
      Tran U, Zakin L, Schweickert A, Agrawal R, Döger R, Blum M, De Robertis EM, Wessely O
      Development  137  1107-16.  2010

      Show Abstract Full Text Article
      20215348 20215348

      Data Sheet

      Title
      RABBIT ANTI-POLYCYSTIN-2 POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies