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MABT1505-100UG Anti-Plakophilin 1 Antibody, clone 10B2

MABT1505-100UG
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT1505-100UG
      DescriptionAnti-Plakophilin 1 Antibody, clone 10B2
      Alternate Names
      • Band 6 protein
      • B6
      Background InformationPlakophilin-1 (UniProt: Q13835; also known as Band 6 protein, B6P) is encoded by the PKP1 gene (Gene ID: 5317) in human. Plakophilins are proteins that are involved in linking adherins to cytoskeletal intermediate filaments. They contain numerous armadillo (ARM) repeats and localize to cell desmosomes and nuclei. Plakophilin-1 may be involved in molecular recruitment and stabilization during desmosome formation. It is also shown to be important for tooth development and regulates ZO-1 distribution in the dental epithelium. Two isoforms of Plankophilin-1 have been reported that are produced by alternative splicing. Isoform 1 is expressed in stratified squamous, complex, glandular duct and bladder epithelia and isoform 2 is widely expressed. Plakophilin-1 is shown to be highly expressed in teeth and skin, and was upregulated during tooth development. It is reported to be involved in the regulation of Wnt signaling via its ARM repeat domains in a manner similar to beta-catenin and Wnt activation induces its nuclear translocation. Mutations n PKP1 gene are shown to cause ectodermal dysplasia-skin fragility syndrome that is characterized by cutaneous fragility and congenital ectodermal dysplasia affecting skin, hair and nails. (Ref.: Sprecher, E., et al (2004). J. Invest. Dermatol. 122(3); 647-51; Miyazaki, K et al. (2016). PLoS ONE 11(3): e0152206).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Plakophilin 1, clone 10B2, Cat. No. MABT1505, is a mouse monoclonal antibody that detects Plantophilin 1 and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Plakophilin 1 in Western Blotting applications (Pigors, M., et. al. (2011). Hum Mol Genet. 20(9):1811-9).

      Immunofluorescence Analysis: A representative lot detected Plakophilin 1 in Immunofluorescence applications (Sprecher, E., et. al. (2004). J Invest Dermatol. 122(3):647-51;Pigors, M., et. al. (2011). Hum Mol Genet. 20(9):1811-9).
      Biological Information
      ImmunogenMBP-conjugated recombinant fragment corresponding to the first 235 amino acids from the N-terminal region of human Plakophilin-1.
      EpitopeN-terminus
      Clone10B2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10B2 specifically detects Planophilin-1 and targets an epitope with in the first 27 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PKP1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~80 kDa observed; 80.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC3 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Plakophilin 1 in 10 µg of PC3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications