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16-199 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate

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16-199
1 mg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIPMGel ImmobilizedMonoclonal Antibody
      Description
      Catalogue Number16-199
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate
      Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
      References
      Product Information
      FormatGel Immobilized
      Control
      • Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
      PresentationGel Immobilized mouse monoclonal antibody covalently coupled to protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.01% Kathon, as a preservative for a total volume of 2 mL. Liquid suspension.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate detects level of Phosphotyrosine & has been published & validated for use in IP.
      Key Applications
      • Immunoprecipitation
      Application NotesThis product is useful for immunoprecipitation and immunoaffinity chromatography methods.
      Biological Information
      EpitopeN/A
      Clone4G10®
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes tyrosine-phosphorylated proteins from all species.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.

      Immunoprecipitation Analysis:
      10 μL of this lot immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC from date of receipt.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate Certificates of Analysis

      TitleLot Number
      Anti-Phosphotyrosine, recombinant -2638024 2638024
      Anti-Phosphotyrosine, recombinant clone 4G10#174;, agarose conjugate - DAM1631998 DAM1631998
      Anti-Phosphotyrosine, recombinant clone 4G10#174;, agarose conjugate - DAM1731744 DAM1731744
      Anti-Phosphotyrosine, recombinant clone 4G10#174;, agarose conjugate - DAM1813280 DAM1813280
      Anti-Phosphotyrosine, recombinant clone 4G10#174;, agarose conjugate - JBC1381998 JBC1381998
      Anti-Phosphotyrosine, recombinant clone 4G10reg;, agarose conjugate - 0609040558 0609040558
      Anti-Phosphotyrosine, recombinant clone 4G10reg;, agarose conjugate - 22463 22463
      Anti-Phosphotyrosine, recombinant clone 4G10®, agarose conjugate - 2420446 2420446
      Anti-Phosphotyrosine, recombinant clone 4G10®, agarose conjugate - 2280865 2280865
      Anti-Phosphotyrosine, recombinant clone 4G10®, agarose conjugate - 3175664 3175664

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies