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16-101 Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate

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16-101
1 mg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtIPMGel ImmobilizedMonoclonal Antibody
      Description
      Catalogue Number16-101
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
      OverviewAnti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate
      Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
      References
      Product Information
      FormatGel Immobilized
      Control
      • Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
      Presentation1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% Kathon ® as a preservative, for a total volume of 2 mL. Liquid suspension.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate is an antibody against Phosphotyrosine for use in IP.
      Key Applications
      • Immunoprecipitation
      Application NotesThis product is useful for immunoprecipitation and immunoaffinity chromatography methods.

      Suitable for immunoprecipitation and affinity purification.
      Biological Information
      ImmunogenPhosphotyramine-KLH.
      Clone4G10®,
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes tyrosine-phosphorylated proteins from all species.
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.

      Immunoprecipitation Analysis:
      10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate Certificates of Analysis

      TitleLot Number
      Anti-Phosphotyrosine (4G10), agarose conjugate - DAM1493362 DAM1493362
      Anti-Phosphotyrosine, clone 4G10#174;, agarose conjugate - 2194171 2194171
      Anti-Phosphotyrosine, clone 4G10#174;, agarose conjugate - 32447 32447
      Anti-Phosphotyrosine, clone 4G10#174;, agarose conjugate - DAM1498551 DAM1498551
      Anti-Phosphotyrosine, clone 4G10#174;, agarose conjugate - DAM1588178 DAM1588178
      Anti-Phosphotyrosine, clone 4G10#174;, agarose conjugate - DAM1658667 DAM1658667
      Anti-Phosphotyrosine, clone 4G10#174;, agarose conjugate - DAM1713310 DAM1713310
      Anti-Phosphotyrosine, clone 4G10#174;, agarose conjugate - DAM1748235 DAM1748235
      Anti-Phosphotyrosine, clone 4G10#174;, agarose conjugate - DAM1770400 DAM1770400
      Anti-Phosphotyrosine, clone 4G10#174;, agarose conjugate - DAM1832570 DAM1832570

      References

      Reference overviewApplicationPub Med ID
      Phosphorylation site dynamics of early T-cell receptor signaling.
      Chylek, LA; Akimov, V; Dengjel, J; Rigbolt, KT; Hu, B; Hlavacek, WS; Blagoev, B
      PloS one  9  e104240  2014

      Show Abstract
      Immunoprecipitation25147952 25147952
      Identification of fibroblast growth factor receptor 3 (FGFR3) as a protein receptor for botulinum neurotoxin serotype A (BoNT/A).
      Jacky, BP; Garay, PE; Dupuy, J; Nelson, JB; Cai, B; Molina, Y; Wang, J; Steward, LE; Broide, RS; Francis, J; Aoki, KR; Stevens, RC; Fernández-Salas, E
      PLoS pathogens  9  e1003369  2013

      Show Abstract
      23696738 23696738
      The neuroblastoma ALK(I1250T) mutation is a kinase-dead RTK in vitro and in vivo.
      Schönherr, C; Ruuth, K; Eriksson, T; Yamazaki, Y; Ottmann, C; Combaret, V; Vigny, M; Kamaraj, S; Palmer, RH; Hallberg, B
      Translational oncology  4  258-65  2011

      Show Abstract
      21804922 21804922
      Identification of oncogenic point mutations and hyperphosphorylation of anaplastic lymphoma kinase in lung cancer.
      Wang, YW; Tu, PH; Lin, KT; Lin, SC; Ko, JY; Jou, YS
      Neoplasia (New York, N.Y.)  13  704-15  2011

      Show Abstract
      Western Blotting21847362 21847362
      Crystal-induced neutrophil activation. IX. Syk-dependent activation of class Ia phosphatidylinositol 3-kinase.
      Popa-Nita, O; Rollet-Labelle, E; Thibault, N; Gilbert, C; Bourgoin, SG; Naccache, PH
      Journal of leukocyte biology  82  763-73  2007

      Show Abstract
      17535983 17535983
      The Mannich base NC1153 promotes long-term allograft survival and spares the recipient from multiple toxicities.
      Stanislaw M Stepkowski, Judy Kao, Mou-Er Wang, Neelam Tejpal, Hemangshu Podder, Lucrezia Furian, Jonathan Dimmock, Amitabh Jha, Umashankar Das, Barry D Kahan, Robert A Kirken
      Journal of immunology (Baltimore, Md. : 1950)  175  4236-46  2005

      Show Abstract
      16177063 16177063
      Regulation of ubiquitin protein ligase activity in c-Cbl by phosphorylation-induced conformational change and constitutive activation by tyrosine to glutamate point mutations.
      Kassenbrock, CK; Anderson, SM
      The Journal of biological chemistry  279  28017-27  2004

      Show Abstract
      15117950 15117950
      Inhibition of Src family kinases blocks epidermal growth factor (EGF)-induced activation of Akt, phosphorylation of c-Cbl, and ubiquitination of the EGF receptor.
      Kassenbrock, CK; Hunter, S; Garl, P; Johnson, GL; Anderson, SM
      The Journal of biological chemistry  277  24967-75  2002

      Show Abstract
      Western Blotting11994282 11994282
      Insulin receptor substrate 4 associates with the protein IRAS.
      Sano, H; Liu, SC; Lane, WS; Piletz, JE; Lienhard, GE
      The Journal of biological chemistry  277  19439-47  2002

      Show Abstract
      11912194 11912194
      Regulated secretion of neurotrophins by metabotropic glutamate group I (mGluRI) and Trk receptor activation is mediated via phospholipase C signalling pathways
      Canossa, M., et al
      Embo J, 20:1640-50 (2001)  2001

      Immunoprecipitation11285228 11285228

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies