Millipore Sigma Vibrant Logo

07-609-I Anti-Peroxiredoxin 1

View Products on Sigmaaldrich.com
07-609-I
100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P), ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-609-I
      Replaces07-609
      DescriptionAnti-Peroxiredoxin 1
      Alternate Names
      • EC:1.11.1.15
      • Natural killer cell-enhancing factor A
      • NKEF-A
      • Proliferation-associated gene protein
      • PAG
      • Thioredoxin peroxidase 2
      • Thioredoxin-dependent peroxide reductase 2
      Background InformationPeroxiredoxin-1 (UniProt: Q06830; also known as EC:1.11.1.15, Natural killer cell-enhancing factor A, NKEF-A, Proliferation-associated gene protein, PAG, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2) is encoded by the PRDX1 (also known as PAGA, PAGB, TDPX2) gene (Gene ID: 5052 ) in human. Peroxiredoxins belong to a family of non-seleno peroxidases. Thus far six different mammalian isoforms have been reported and they all catalyze peroxide reduction of hydrogen peroxide, organic hydroperoxides and peroxynitrite. All peroxiredoxins share a conserved Cys residue that corresponds to the N-terminal Cys51 in mammalian peroxiredoxin-1. Peroxiredoxin 1-4 also contain an additional conserved Cys residue in the C-terminal region, Cys172. The N-terminal Cys51 is shown to be oxidized by hydrogen peroxide to form cysteine-sulfenic acid (Cys51-SOH) and reacts with Cys172-SH of the other subunit to produce an intermolecular disulfide, which can only be reduced by thioredoxin and not by glutathione or glutaredoxin. Peroxiredoxin-1 is constitutively expressed in most human cells and is induced to higher levels upon serum stimulation in cells. It is involved in redox regulation of the cell and important role in overcoming oxidative stress. Peroxiredoxin-1 also eliminates peroxides generated during drug metabolism. It is phosphorylated on Thr90 during the M-phase of cell cycle, which leads to over 80% reduction in its enzymatic activity.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Peroxiredoxin 1 Antibody, Cat. No. 07-609-I, is a highly specific rabbit polyclonal antibody that targets Peroxiredoxin 1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Peroxiredoxin 1 in HeLa cells.

      Immunohistochemistry Analysis: A 1:250-1:1,000 dilution from a representative lot detected Peroxiredoxin 1 in human kidney, human small intestine, and human cerebellum tissues.
      Biological Information
      ImmunogenKLH-conjugated linnear peptide corresponding to 12 amino acids from the C-terminal region of human Peroxiredoxin-1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects Peroxiredoxin-1 by Western blotting and immunocytochemistry in in DU145 cells and HeLa cells, respectively.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with various other mammalian species, including Bat, Canine, Hamster, Rat, and Rhesus Monkey.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRDX1
      • PAGA
      • PAGB
      • TDPX2
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~21 kDa observed; 22.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in DU145 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Peroxiredoxin 1 in 10 µg of DU145 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications