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07-330 Anti-Partitioning-defective 3 Antibody

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07-330
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MICC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-330
      ReplacesMABS174
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Partitioning-defective 3 Antibody
      Alternate Names
      • Atypical PKC isotype-specific-interacting protein
      • CTCL tumor antigen se2-5
      • atypical PKC isotype-specific interacting protein
      • par-3 (partitioning defective 3, C.elegans) homolog
      • par-3 partitioning defective 3 homolog (C. elegans)
      • partitioning-defective protein 3 homolog
      Background InformationPartitioning defective 3 homolog (UniProt: Q99NH2; also known as PAR-3, PARD-3, Atypical PKC isotype-specific-interacting protein, ASIP, Ephrin-interacting protein, PHIP) is encoded by the Pard3 (also known as Par3) gene (Gene ID: 93742) in murine species. PAR-3 is an adapter protein that is involved in asymmetrical cell division and cell polarization processes. It plays a vital role in the formation of epithelial tight junctions and targets PTEN to cell junctions. Its association with PAR-6 may prevent the interaction of PAR-3 with F11 receptor/junctional adhesion molecule (F11R/JAM1), thereby preventing tight junction assembly. The PAR-3-PAR-6 complex also links GTP-bound Rho to atypical protein kinase C. PAR-3 is shown to be essential for establishment of neuronal polarity and normal axon formation. It is also involved in Schwann cell peripheral myelination where its deacetylation by Sirt2 reduces the activity of the polarity complex signaling component aPKC. In premyelinating Schwann cells, PAR-3 interacts with p75NTR through its PDZ1 binding domain to re-localize this receptor to axon-glial junctions, thereby promoting in vitro myelination. PAR-3 phosphorylation at serine 824 by PKC and PKC is shown to occur at the most apical tip of epithelial cell-cell contacts during the initial phase of tight junction formation. EGF-induced phosphorylation of tyrosine 1123 mediates its dissociation from (LIM Domain Kinase 2 ( LIMK2). Five isoforms of PAR-3 have been described that are produced by alternative splicing. All isoforms are expressed in the heart; however, its expression in brain is mainly limited to isoform 1. (Ref.: Beirowski, B., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(43); E952-E961; Gao, L., et al. (2002). Curr. Biol. 12(3); 221-225).
      References
      Product Information
      FormatAffinity Purified
      Control
      • 3T3/A31 lysate.

        Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 lysate. Add 2.5 µL of 2-mercapto-ethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PAR-3, Cat. No. 07-330 rabbit polyclonal antibody that detects PAR-3 and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesTested Applications

      Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected PAR-3 in NIH 3T3 cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 288 amino acids from the internal region of mouse Partitioning defective 3 homolog (PAR-3).
      EpitopeInternal
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Partitioning defective 3 homolog (PAR-3). It targets an epitope within the internal region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Monkey, Rat, Human, Canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPARD proteins, which were first identified in C. elegans, are essential for asymmetric cell division and polarized growth, whereas CDC42 (MIM 116952) mediates the establishment of cell polarity. The CDC42 GTPase, which is controlled by nucleotide exchange factors (GEFs; see MIM 606057) and GTPase-activating proteins (GAPs; see MIM 604980), interacts with a large set of effector proteins that typically contain a CDC42/RAC (MIM 602048)-interactive binding (CRIB) domain.[supplied by OMIM]
      Gene Symbol
      • PAR3
      • PARD3
      • ASIP
      • PHIP
      • SE2-5T2
      • PAR-3
      • SE2-5L16
      • PARD-3
      • PARD3A
      • PAR3A
      • PAR3alpha
      • PAR3-alpha
      • Bazooka
      • FLJ21015
      • Baz
      • SE2-5LT1
      Purification MethodAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8TEW0 # Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
      SIZE: 1356 amino acids; 151423 Da
      SUBUNIT: Interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. Interacts with PRCKI (By similarity). Part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. Interacts with F11R/JAM1 (By similarity). Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3.
      SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cell junction. Cell membrane. Note=Partially localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions.
      TISSUE SPECIFICITY: Widely expressed.
      PTM: Phosphorylated by PRKCZ.
      SIMILARITY: SwissProt: Q8TEW0 ## Belongs to the PAR3 family. & Contains 3 PDZ (DHR) domains.
      MISCELLANEOUS: Antibodies against PARD3 are present in sera from patients with cutaneous T-cell lymphomas.
      Molecular Weight180, 150, and 95 kDa observed; 149.08, 113.5 kDa ,and 98.74 kDa calculated, respectively for isoforms 1, 2, and 4. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Balb3T3 clone A31 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected PAR-3 in Balb3T3 clone A31 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      07-330 04053252324468

      Documentation

      Anti-Partitioning-defective 3 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Partitioning-defective 3 Antibody Certificates of Analysis

      TitleLot Number
      Anti-PAR-3 (rabbit polyclonal IgG) - 2193160 2193160
      Anti-Partitioning-defective 3 3064838
      Anti-Partitioning-defective 3 - 2113356 2113356
      Anti-Partitioning-defective 3 - 2322514 2322514
      Anti-Partitioning-defective 3 - 2391109 2391109
      Anti-Partitioning-defective 3 - 1962116 1962116
      Anti-Partitioning-defective 3 - 2016672 2016672
      Anti-Partitioning-defective 3 - 2044180 2044180
      Anti-Partitioning-defective 3 - 3122919 3122919
      Anti-Partitioning-defective 3 - 3247098 3247098

      References

      Reference overviewApplicationSpeciesPub Med ID
      RFX2 Is a Major Transcriptional Regulator of Spermiogenesis.
      Kistler, WS; Baas, D; Lemeille, S; Paschaki, M; Seguin-Estevez, Q; Barras, E; Ma, W; Duteyrat, JL; Morlé, L; Durand, B; Reith, W
      PLoS genetics  11  e1005368  2015

      Show Abstract
      26162102 26162102
      Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
      Poitelon, Y; Bogni, S; Matafora, V; Della-Flora Nunes, G; Hurley, E; Ghidinelli, M; Katzenellenbogen, BS; Taveggia, C; Silvestri, N; Bachi, A; Sannino, A; Wrabetz, L; Feltri, ML
      Nature communications  6  8303  2015

      Show Abstract
      Immunohistochemistry26383514 26383514
      Wdr1-mediated cell shape dynamics and cortical tension are essential for epidermal planar cell polarity.
      Luxenburg, C; Heller, E; Pasolli, HA; Chai, S; Nikolova, M; Stokes, N; Fuchs, E
      Nature cell biology  17  592-604  2015

      Show Abstract
      25915128 25915128
      Type VII collagen regulates expression of OATP1B3, promotes front-to-rear polarity and increases structural organisation in 3D spheroid cultures of RDEB tumour keratinocytes.
      Dayal, JH; Cole, CL; Pourreyron, C; Watt, SA; Lim, YZ; Salas-Alanis, JC; Murrell, DF; McGrath, JA; Stieger, B; Jahoda, C; Leigh, IM; South, AP
      Journal of cell science  127  740-51  2014

      Show Abstract
      24357722 24357722
      Dual function of Yap in the regulation of lens progenitor cells and cellular polarity.
      Song, JY; Park, R; Kim, JY; Hughes, L; Lu, L; Kim, S; Johnson, RL; Cho, SH
      Developmental biology  386  281-90  2014

      Show Abstract
      24384391 24384391
      Developmental stratification of the mammary epithelium occurs through symmetry-breaking vertical divisions of apically positioned luminal cells.
      Huebner, RJ; Lechler, T; Ewald, AJ
      Development (Cambridge, England)  141  1085-94  2014

      Show Abstract
      24550116 24550116
      Par3-mInsc and Gαi3 cooperate to promote oriented epidermal cell divisions through LGN.
      Williams, SE; Ratliff, LA; Postiglione, MP; Knoblich, JA; Fuchs, E
      Nature cell biology  16  758-69  2014

      Show Abstract
      25016959 25016959
      Expression of partitioning defective 3 (Par-3) for predicting extrahepatic metastasis and survival with hepatocellular carcinoma.
      Jan, YJ; Ko, BS; Liu, TA; Wu, YM; Liang, SM; Chen, SC; Wang, J; Liou, JY
      International journal of molecular sciences  14  1684-97  2013

      Show Abstract
      23322019 23322019
      aPKCλ/ι and aPKCζ contribute to podocyte differentiation and glomerular maturation.
      Hartleben, B; Widmeier, E; Suhm, M; Worthmann, K; Schell, C; Helmstädter, M; Wiech, T; Walz, G; Leitges, M; Schiffer, M; Huber, TB
      Journal of the American Society of Nephrology : JASN  24  253-67  2013

      Show Abstract
      23334392 23334392
      Atypical protein kinase C and Par3 are required for proteoglycan-induced axon growth inhibition.
      Lee, SI; Zhang, W; Ravi, M; Weschenfelder, M; Bastmeyer, M; Levine, JM
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  2541-54  2013

      Show Abstract
      23392682 23392682

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies