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07-330 Anti-Partitioning-defective 3 Antibody

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07-330
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Mk, M, CaICC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-330
      ReplacesMABS174
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Partitioning-defective 3 Antibody
      Alternate Names
      • Atypical PKC isotype-specific-interacting protein
      • CTCL tumor antigen se2-5
      • atypical PKC isotype-specific interacting protein
      • par-3 (partitioning defective 3, C.elegans) homolog
      • par-3 partitioning defective 3 homolog (C. elegans)
      • partitioning-defective protein 3 homolog
      Background InformationPAR3 is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR3 interacts with PRCKI. PAR3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR3 interacts with F11R/JAM1. Antibodies against PAR3 are present in sera from patients with cutaneous T cell lymphomas.
      References
      Product Information
      FormatPurified
      Control
      • 3T3/A31 lysate.

        Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 lysate. Add 2.5 µL of 2-mercapto-ethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
      PresentationPurified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Partitioning-defective 3 Antibody is an antibody against Partitioning-defective 3 and has been published in over 20 citations for use in IC and WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      A previous lot of this antibody showed positive immunostaining for PAR-3 in MDCK cells fixed with 3% paraformaldehyde followed by permeabilization with 0.1% Triton X-100 has been shown by an independent laboratory.
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes all 3 slice isoforms of PAR-3, MW ~180, 150, 100 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Monkey
      • Mouse
      • Canine
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryPARD proteins, which were first identified in C. elegans, are essential for asymmetric cell division and polarized growth, whereas CDC42 (MIM 116952) mediates the establishment of cell polarity. The CDC42 GTPase, which is controlled by nucleotide exchange factors (GEFs; see MIM 606057) and GTPase-activating proteins (GAPs; see MIM 604980), interacts with a large set of effector proteins that typically contain a CDC42/RAC (MIM 602048)-interactive binding (CRIB) domain.[supplied by OMIM]
      Gene Symbol
      • PAR3
      • PARD3
      • ASIP
      • PHIP
      • SE2-5T2
      • PAR-3
      • SE2-5L16
      • PARD-3
      • PARD3A
      • PAR3A
      • PAR3alpha
      • PAR3-alpha
      • Bazooka
      • FLJ21015
      • Baz
      • SE2-5LT1
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8TEW0 # Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
      SIZE: 1356 amino acids; 151423 Da
      SUBUNIT: Interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. Interacts with PRCKI (By similarity). Part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. Interacts with F11R/JAM1 (By similarity). Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3.
      SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cell junction. Cell membrane. Note=Partially localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions.
      TISSUE SPECIFICITY: Widely expressed.
      PTM: Phosphorylated by PRKCZ.
      SIMILARITY: SwissProt: Q8TEW0 ## Belongs to the PAR3 family. & Contains 3 PDZ (DHR) domains.
      MISCELLANEOUS: Antibodies against PARD3 are present in sera from patients with cutaneous T-cell lymphomas.
      Molecular Weight~180, 150 & 100 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates from 3T3/A31, A431, and HeLa cells.

      Western Blot Analysis:
      0.5-2 µg/mL of this antibody detected PAR-3 isoforms in RIPA lysates from 3T3/A31, A431, and HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Partitioning-defective 3 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Partitioning-defective 3 Antibody Certificates of Analysis

      TitleLot Number
      Anti-PAR-3 (rabbit polyclonal IgG) - 2193160 2193160
      Anti-Partitioning-defective 3 3064838
      Anti-Partitioning-defective 3 - 2113356 2113356
      Anti-Partitioning-defective 3 - 2322514 2322514
      Anti-Partitioning-defective 3 - 2391109 2391109
      Anti-Partitioning-defective 3 - 1962116 1962116
      Anti-Partitioning-defective 3 - 2016672 2016672
      Anti-Partitioning-defective 3 - 2044180 2044180
      Anti-Partitioning-defective 3 - 3122919 3122919
      Anti-Partitioning-defective 3 - 3247098 3247098

      References

      Reference overviewApplicationSpeciesPub Med ID
      Wdr1-mediated cell shape dynamics and cortical tension are essential for epidermal planar cell polarity.
      Luxenburg, C; Heller, E; Pasolli, HA; Chai, S; Nikolova, M; Stokes, N; Fuchs, E
      Nature cell biology  17  592-604  2015

      Show Abstract
      25915128 25915128
      RFX2 Is a Major Transcriptional Regulator of Spermiogenesis.
      Kistler, WS; Baas, D; Lemeille, S; Paschaki, M; Seguin-Estevez, Q; Barras, E; Ma, W; Duteyrat, JL; Morlé, L; Durand, B; Reith, W
      PLoS genetics  11  e1005368  2015

      Show Abstract
      26162102 26162102
      Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
      Poitelon, Y; Bogni, S; Matafora, V; Della-Flora Nunes, G; Hurley, E; Ghidinelli, M; Katzenellenbogen, BS; Taveggia, C; Silvestri, N; Bachi, A; Sannino, A; Wrabetz, L; Feltri, ML
      Nature communications  6  8303  2015

      Show Abstract
      Immunohistochemistry26383514 26383514
      Par3-mInsc and Gαi3 cooperate to promote oriented epidermal cell divisions through LGN.
      Williams, SE; Ratliff, LA; Postiglione, MP; Knoblich, JA; Fuchs, E
      Nature cell biology  16  758-69  2014

      Show Abstract
      25016959 25016959
      Type VII collagen regulates expression of OATP1B3, promotes front-to-rear polarity and increases structural organisation in 3D spheroid cultures of RDEB tumour keratinocytes.
      Dayal, JH; Cole, CL; Pourreyron, C; Watt, SA; Lim, YZ; Salas-Alanis, JC; Murrell, DF; McGrath, JA; Stieger, B; Jahoda, C; Leigh, IM; South, AP
      Journal of cell science  127  740-51  2014

      Show Abstract
      24357722 24357722
      Developmental stratification of the mammary epithelium occurs through symmetry-breaking vertical divisions of apically positioned luminal cells.
      Huebner, RJ; Lechler, T; Ewald, AJ
      Development (Cambridge, England)  141  1085-94  2014

      Show Abstract
      24550116 24550116
      Dual function of Yap in the regulation of lens progenitor cells and cellular polarity.
      Song, JY; Park, R; Kim, JY; Hughes, L; Lu, L; Kim, S; Johnson, RL; Cho, SH
      Developmental biology  386  281-90  2014

      Show Abstract
      24384391 24384391
      PKCζ mediates breakdown of outer blood-retinal barriers in diabetic retinopathy.
      Omri, S; Behar-Cohen, F; Rothschild, PR; Gélizé, E; Jonet, L; Jeanny, JC; Omri, B; Crisanti, P
      PloS one  8  e81600  2013

      Show Abstract
      24312324 24312324
      Par-complex aPKC and Par3 cross-talk with innate immunity NF-κB pathway in epithelial cells.
      Forteza, R; Wald, FA; Mashukova, A; Kozhekbaeva, Z; Salas, PJ
      Biology open  2  1264-9  2013

      Show Abstract
      24244864 24244864
      Loss of aPKCλ in differentiated neurons disrupts the polarity complex but does not induce obvious neuronal loss or disorientation in mouse brains.
      Yamanaka, T; Tosaki, A; Kurosawa, M; Akimoto, K; Hirose, T; Ohno, S; Hattori, N; Nukina, N
      PloS one  8  e84036  2013

      Show Abstract
      24391875 24391875

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies