OP38 Anti-Pan-Ras (Ab-1) Mouse mAb (DWP)

OP38
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

Pricing & Availability

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OP38-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~21 kDa H-, K-, and N-Ras proteins.
      Catalogue NumberOP38
      Brand Family Calbiochem®
      References
      ReferencesHamer, P.J., et al. 1991 Oncogene 6, 1609.
      Ng, S., et al. 1991. Hybridoma 10, 467.
      Hamer, P.J. et al. 1990. Hybridoma 9, 573.
      Pullano, T.G., et al. 1989. Oncogene 4, 1003.
      Bos, J.L., 1988. Mutation Res. 195, 255.
      Furth, M.E., et al. 1987. Oncogene 1, 47.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlK-Ras Val¹² Protein (Cat. No. WA04)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone
      Carney, W.P. et al. 1986. Proc. Natl. Acad. Sci., USA 83, 7485.
      Specificity
      Wang, Z., et al. 1997. J. Peptide Res. 50, 483.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Application CommentsRecognizes H-, K-, and N-Ras proteins. Per Wang, Z., et al. 1997, this clone may also react with other Ras mutants with a hydrophobic amino acid (V, A, C, H, I, L, M, Y, T, N, Q) at position 12. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 5-16 of Ras (H, K, or N) with Val at position 12
      CloneDWP
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Pan-Ras (Ab-1) Mouse mAb (DWP) Certificates of Analysis

      TitleLot Number
      OP38

      References

      Reference overview
      Hamer, P.J., et al. 1991 Oncogene 6, 1609.
      Ng, S., et al. 1991. Hybridoma 10, 467.
      Hamer, P.J. et al. 1990. Hybridoma 9, 573.
      Pullano, T.G., et al. 1989. Oncogene 4, 1003.
      Bos, J.L., 1988. Mutation Res. 195, 255.
      Furth, M.E., et al. 1987. Oncogene 1, 47.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-September-2010 JSW
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c x C57BL/6 mice with the specified immunogen and fusing splenocytes with Sp2/0 myeloma cells (see application references). Recognizes the ~21 kDa Ras protein.
      BackgroundThe human ras gene family consists of three identified members H, K and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons weight. Human H-and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and its relevance to human disease diagnosis and prognosis remains to be determined.
      HostMouse
      Immunogena synthetic peptide corresponding to amino acids 5-16 of Ras (H, K, or N) with Val at position 12
      CloneDWP
      IsotypeIgG2b
      Specieshuman, mouse, rat
      Positive controlK-Ras Val¹² Protein (Cat. No. WA04)
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsRecognizes H-, K-, and N-Ras proteins. Per Wang, Z., et al. 1997, this clone may also react with other Ras mutants with a hydrophobic amino acid (V, A, C, H, I, L, M, Y, T, N, Q) at position 12. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesHamer, P.J., et al. 1991 Oncogene 6, 1609.
      Ng, S., et al. 1991. Hybridoma 10, 467.
      Hamer, P.J. et al. 1990. Hybridoma 9, 573.
      Pullano, T.G., et al. 1989. Oncogene 4, 1003.
      Bos, J.L., 1988. Mutation Res. 195, 255.
      Furth, M.E., et al. 1987. Oncogene 1, 47.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Application referencesOriginal Clone
      Carney, W.P. et al. 1986. Proc. Natl. Acad. Sci., USA 83, 7485.
      Specificity
      Wang, Z., et al. 1997. J. Peptide Res. 50, 483.