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MABC942 Anti-PLA2R Antibody, clone 5F5.1

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MABC942
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC942
      DescriptionAnti-PLA2R Antibody, clone 5F5.1
      Alternate Names
      • PLA2-R
      • PLA2R
      • Secretory phospholipase A2 receptor
      • 180 kDa secretory phospholipase A2 receptor
      • C-type lectin domain family 13 member C
      • M-type receptor
      Background InformationSecretory phospholipase A2 receptor (UniProt Q13018; also known as 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13 member C, M-type receptor, Phospholipase A2 receptor 1) is encoded by the PLA2R1 (also known as CLEC13C, PLA2G1R, PLA2R, PLA2IR) gene (Gene ID 22925) in human. PLA2R is initially produced with a signal peptide sequence (a.a. 1-20), the cleavage of which yields the mature type I transmembrane receptor composed of a short cytoplasmic tail (a.a. 1419-1463), a transmembrane segment (a.a. 1419-1463), and a large extracellular portion consisting of an N-terminal cystein-rich region, a fibronectin-like type II domain, and a tandem repeat of eight carbohydrate-recognition domains (CRDs). Both group IB and X secretory phospholipase A2 (sPLA2) enzymes are identified as ligands orf PLA2R, while group IIA sPLA2s bind PLA2R with about 10-fold lower affinity when compared with group IB and X sPLA2s. In mice, the extracellular portion of PLA2R is reported to be cleaved off into a soluble form (sPLA2R) in blood, where it functions as a circulating sPLA2 inhibitory protein (PLI) that blocks both the receptor-binding and enzymatic activity of sPLA2.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-PLA2R Antibody, clone 5F5.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of PLA2R.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected PLA2R in 10 µg of human kidney tissue lysate.
      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PLA2R in human kidney tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the extracellular C-type lectin domains 3-5 of human PLA2R.
      EpitopeExtracellular C-type lectin domains 3-5
      Clone5F5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with both spliced isoforms, as well as the soluble receptor (sPLA2R) derived from metalloproteinases-mediated cleavage of the membrane-bound receptor.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PLA2R1
      • CLEC13C
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~180 kDa observed. Target band(s) appears larger than the calculated molecular weights (166.6 kDa/isoform 1 and 150.8 kDa/isoform 2) due to posttranslational glycosylation. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human lung tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected PLA2R in 10 µg of human lung tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-PLA2R Antibody, clone 5F5.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PLA2R Antibody, clone 5F5.1 Certificates of Analysis

      TitleLot Number
      Anti-PLA2R, clone 5F5.1 - 2986013 2986013
      Anti-PLA2R, clone 5F5.1 - 3245601 3245601
      Anti-PLA2R, clone 5F5.1 - 3316667 3316667
      Anti-PLA2R, clone 5F5.1 - 3681231 3681231
      Anti-PLA2R, clone 5F5.1 -Q2614585 Q2614585
      Anti-PLA2R, clone 5F5.1 Monoclonal Antibody 3122249

      Brochure

      Title
      New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody (EMD)

      Posters

      Title
      Post-Translational Modifications