ABC439 Anti-PKM1 Antibody

View Products on Sigmaaldrich.com
ABC439
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H WB, IHC Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberABC439
      DescriptionAnti-PKM1 Antibody
      Alternate Names
      • Pyruvate kinase PKM
      • Cytosolic thyroid hormone-binding protein
      • CTHBP
      • Opa-interacting protein 3
      • OIP-3
      • Pyruvate kinase 2/3
      • Pyruvate kinase muscle isozyme
      • Thyroid hormone-binding protein 1
      • THBP1
      • Tumor M2-PK
      • p58
      Background InformationPyruvate kinase isozymes M1/M2 (PKM2) is also called Cytosolic thyroid hormone-binding protein (CTHBP), Opa-interacting protein 3 (OIP-3), Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1 (THBP1) and p58. PKM2 is a glycolytic enzyme that assists in generating ATP. PKM2 also stimulates POU5F1-mediated transcriptional activation, and plays a role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. This control of glycolysis and is important for tumor cell proliferation and survival. PKM2 is specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, and cancer cells.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PKM1 Antibody detects level of PKM1 & has been published & validated for use in PKM1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected PKM1 in 10 µg of mouse brain and RAW264.7 tissue lysate.
      Western Blotting Analysis: A representative lot detected PKM1 in HEK293 cell lysate (Christofk, R. H., et al. (2008). Nature. 452:230-233).
      Immunohistochemistry Analysis: A representative lot detected PKM1 in HEK293 tissue (Christofk, R. H., et al. (2008). Nature. 452:230-233).
      Biological Information
      ImmunogenLinear peptide corresponding to human PKM1.
      HostRabbit
      SpecificityThis anitbody is specific to PKM1.
      Species Reactivity
      • Mouse
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PKM
      • OIP3
      • PK2
      • PK3
      • PKM2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~58 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in C2C12 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PKM1 in 10 µg of C2C12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-PKM1 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PKM1 Antibody Certificates of Analysis

      TitleLot Number
      Anti-PKM1 - Q2428568Q2428568
      Anti-PKM1 - 34228263422826

      References

      Reference overviewPub Med ID
      The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.
      Christofk, Heather R, et al.
      Nature, 452: 230-3 (2008)  2008

      Show Abstract
      18337823 18337823

      Related Products & Applications

      Related Products

      Catalog Number Description  
      ABC438 Anti-PKM2 Antibody Show Pricing & Availability
      ABS245 Anti-PKM2 Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies