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MAB1398Z Anti-PECAM-1 Antibody, clone 2H8, Azide Free

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, CaEM, FC, ICC, IF, IHC, IP, Neutralization AssayAHmPurifiedMonoclonal Antibody
      Catalogue NumberMAB1398Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-PECAM-1 Antibody, clone 2H8, Azide Free
      Alternate Names
      • CD31
      • PECAM-1
      • Platelet endothelial cell adhesion molecule
      Background InformationCD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets.

      CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
      Product Information
      • Mouse cell lung tissue
      PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, containing no preservatives.
      Quality LevelMQ100
      ApplicationAnti-PECAM-1 Antibody, clone 2H8, Azide Free detects level of PECAM-1 & has been published & validated for use in Elecron Microscopy, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Neutralization Assay.
      Key Applications
      • Electron Microscopy
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Neutralization Assay
      Application NotesImmunofluorescence Analysis: Representative lots immunostained endothelial blood vessel cells in 4% paraformaldehyde-fixed frozen tumor sections from mice and dogs as a means for assessing lymph vessel density in tumor xenografts by fluorescent immunohistochemistry (Donat, U., et al. (2012). PLoS One. 7(9):e45942; Gentschev, I., et al. (2012). PLoS One.7(5):e37239).
      Immunofluorescence Analysis: A representative lot immunostained endothelial cells in formalin-fixed, paraffin-embedded murine peritoneum sections by fluorescent immunohistochemistry (Tanabe, K., et al. (2007). Kidney Int. 71(3):227-238).
      Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed endothelial cells differentiated from murine ESCs in cultures (Purpura, K.A., et al. (2008). Stem Cells. 26(11):2832-2842).
      Immunocytochemistry Analysis: Clone 2H8 immunostained 3% paraformaldehyde-fixed, 0.5% NP-40-permeabilized COS-7 transfectants expressing murine, but not human, CD31 by fluorescent immunocytochemistry (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
      Neutralization Analysis: A representative lot, when injected intravenously in mice, blocked leukocyte emigration in response to thioglycollate injection (Bogen, S., et al. (1994). J. Exp. Med. 179(3):1059-1064).
      Neutralization Analysis: A representative lot blocked murine PECAM-mediated aggregation of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
      Flow Cytometry Analysis: A representative lot detected the expression of exogenously transfected murine CD31 on the surface of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
      Flow Cytometry Analysis: Clone 2H8 detected CD31 immunoreactivity on the surface of various murine B- and T-cell lines (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed murine, but not human, CD31 from COS-7 transfectants (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
      Immunoprecipitation Analysis: Clone 2H8 immunoprecipitated CD31 from murine CTLL T lymphocytes and Sp2/0 B lymphocytes (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
      Electron Microscopy Analysis: Clone 2H8 was biotinylated and immunostained lymphocytes in 0.01% glutaraldehyde-/2% formaldehyde-fxed frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
      Immunohistochemistry Analysis: Clone 2H8 immunostained lymphocytes adherent to the sinusoidal and postcapillary venular endothelium of frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
      Biological Information
      ImmunogenD10.G4.1 murine T cells (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843 -854).
      EpitopeExtracellular domain.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostArmenian Hamster
      SpecificityReact s with the 130-140 kDa protein, PECAM-1 found on mouse monocytes, platelets, granulocytes and endothelial cells. 2H8 effectively inhibits transmigration of activated polymophonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonis - 2H8 blocks acute inflammation.
      Species Reactivity
      • Mouse
      • Canine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pecam
      • Pecam-1
      • Pecam1
      Purification MethodProtein A Purfied
      UniProt Number
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-PECAM-1 Antibody, clone 2H8, Azide Free SDS


      Safety Data Sheet (SDS) 

      Anti-PECAM-1 Antibody, clone 2H8, Azide Free Certificates of Analysis

      TitleLot Number
      HAMSTER ANTI-MOUSE CD31 - 3170789 3170789
      HAMSTER ANTI-MOUSE CD31 - 3195944 3195944
      HAMSTER ANTI-MOUSE CD31 - 3211892 3211892
      HAMSTER ANTI-MOUSE CD31 - 3229347 3229347


      Reference overviewApplicationSpeciesPub Med ID
      Systemic inflammation in early neonatal mice induces transient and lasting neurodegenerative effects.
      Cardoso, FL; Herz, J; Fernandes, A; Rocha, J; Sepodes, B; Brito, MA; McGavern, DB; Brites, D
      Journal of neuroinflammation  12  82  2015

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      25924675 25924675
      Preclinical Testing Oncolytic Vaccinia Virus Strain GLV-5b451 Expressing an Anti-VEGF Single-Chain Antibody for Canine Cancer Therapy.
      Adelfinger, M; Bessler, S; Frentzen, A; Cecil, A; Langbein-Laugwitz, J; Gentschev, I; Szalay, AA
      Viruses  7  4075-92  2015

      Show Abstract
      26205404 26205404
      Degeneration and regeneration of corneal nerves in response to HSV-1 infection.
      Chucair-Elliott, AJ; Zheng, M; Carr, DJ
      Investigative ophthalmology & visual science  56  1097-107  2015

      Show Abstract
      25587055 25587055
      Endothelial destabilization by angiopoietin-2 via integrin β1 activation.
      Hakanpaa, L; Sipila, T; Leppanen, VM; Gautam, P; Nurmi, H; Jacquemet, G; Eklund, L; Ivaska, J; Alitalo, K; Saharinen, P
      Nature communications  6  5962  2015

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      Immunofluorescence25635707 25635707
      Local acting Sticky-trap inhibits vascular endothelial growth factor dependent pathological angiogenesis in the eye.
      Michael, IP; Westenskow, PD; Hacibekiroglu, S; Greenwald, AC; Ballios, BG; Kurihara, T; Li, Z; Warren, CM; Zhang, P; Aguilar, E; Donaldson, L; Marchetti, V; Baba, T; Hussein, SM; Sung, HK; Iruela-Arispe, ML; Rini, JM; van der Kooy, D; Friedlander, M; Nagy, A
      EMBO molecular medicine  6  604-23  2014

      Show Abstract
      24705878 24705878
      The Schlemm's canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel.
      Aspelund, A; Tammela, T; Antila, S; Nurmi, H; Leppänen, VM; Zarkada, G; Stanczuk, L; Francois, M; Mäkinen, T; Saharinen, P; Immonen, I; Alitalo, K
      The Journal of clinical investigation  124  3975-86  2014

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      ImmunofluorescenceMouse25061878 25061878
      Formulation optimization and in vivo proof-of-concept study of thermosensitive liposomes balanced by phospholipid, elastin-like polypeptide, and cholesterol.
      Park, SM; Cha, JM; Nam, J; Kim, MS; Park, SJ; Park, ES; Lee, H; Kim, HR
      PloS one  9  e103116  2014

      Show Abstract
      25068721 25068721
      Nerve growth factor regulates neurolymphatic remodeling during corneal inflammation and resolution.
      Fink, DM; Connor, AL; Kelley, PM; Steele, MM; Hollingsworth, MA; Tempero, RM
      PloS one  9  e112737  2014

      Show Abstract
      25383879 25383879
      Ocular phenotype of Fbn2-null mice.
      Shi, Y; Tu, Y; Mecham, RP; Bassnett, S
      Investigative ophthalmology & visual science  54  7163-73  2013

      Show Abstract
      24130178 24130178
      Mitochondrial oxidative phosphorylation reserve is required for hormone- and PPARγ agonist-induced adipogenesis.
      Ryu, MJ; Kim, SJ; Choi, MJ; Kim, YK; Lee, MH; Lee, SE; Chung, HK; Jung, SB; Kim, HJ; Kim, KS; Jo, YS; Kweon, GR; Lee, CH; Shong, M
      Molecules and cells  35  134-41  2013

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      23456335 23456335

      Data Sheet


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