NA03 Anti-PCNA (Ab-1) Mouse mAb (PC10)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R, Yeast, Zebrafish M Monoclonal Antibody

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NA03-200UG
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      Plastic ampoule 200 μg
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      Description
      OverviewRecognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
      Catalogue NumberNA03
      Brand Family Calbiochem®
      SynonymsAnti-Proliferating Cell Nuclear Antigen
      References
      ReferencesWaseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121.
      Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189.
      Bravo, R., et al. 1987. Nature (London) 326, 515.
      Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
      Miyachi, K., et al. 1987. J. Immunol. 121, 2228.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlHeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Paraffin Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Not Immunoprecipitation
      Application NotesImmunoblotting (2.5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (not recommended with unconjugated antibody, see comments)
      Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
      Application CommentsStaining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant PCNA
      EpitopeWithin amino acids 112-121
      ClonePC10
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Yeast
      • Zebrafish
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-PCNA (Ab-1) Mouse mAb (PC10) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PCNA (Ab-1) Mouse mAb (PC10) Certificates of Analysis

      TitleLot Number
      NA03

      References

      Reference overview
      Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121.
      Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189.
      Bravo, R., et al. 1987. Nature (London) 326, 515.
      Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
      Miyachi, K., et al. 1987. J. Immunol. 121, 2228.

      Citations

      Title
      Goessling, et al. 2008. Developmental Biology 320, 161.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-September-2007 RFH
      SynonymsAnti-Proliferating Cell Nuclear Antigen
      ApplicationImmunoblotting (2.5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (not recommended with unconjugated antibody, see comments)
      Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
      BackgroundThe proliferating cell nuclear antigen (PCNA) is a 37 kDa molecular weight protein also known as cyclin. The protein has also been identified as the polymerase δ accessory protein and is detected in a cell cycle dependent manner. In early S phase, PCNA has a very granular distribution and is absent from the nucleoli. At late S phase, PCNA is prominent in the nucleoli. In cells fixed with organic solvents, the PCNA is seen to be strongly associated in the nuclear regions where DNA synthesis is occurring, whereas in cells fixed with aldehydes, the staining is more diffuse but intense and occurs throughout the cell cycle. This is due to the presence of two basic forms of the PCNA protein: a soluble form which is sensitive to organic fixation and not involved in replication and a second form which is insoluble and associated with on-going DNA synthesis. PCNA is a very conserved protein present not only in mammals but also in plant cells. Patients suffering from SLE have been shown to have auto antibodies against PCNA.
      HostMouse
      Immunogenrecombinant PCNA
      EpitopeWithin amino acids 112-121
      ClonePC10
      IsotypeIgG2a
      Specieshuman, mouse, rat, yeast, zebrafish
      Positive controlHeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsStaining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesWaseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121.
      Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189.
      Bravo, R., et al. 1987. Nature (London) 326, 515.
      Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
      Miyachi, K., et al. 1987. J. Immunol. 121, 2228.
      CitationGoessling, et al. 2008. Developmental Biology 320, 161.