Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
Catalog Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Species
Panel Type
Selected Kit
Qty
Catalog Number
Ordering Description
Qty/Pack
List Price
96-Well Plate
Qty
Catalog Number
Ordering Description
Qty/Pack
List Price
Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
Qty
Catalog Number
Ordering Description
Qty/Pack
List Price
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
This item has been added to favorites.
The Product Has Been Added To Your Cart
You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
The quantity field is empty. Please enter a quantity of 1 or more to add items to your cart.
Description
Overview
Recognizes the P2X7 receptor protein. Supplied with a control peptide.
Catalogue Number
506107
Brand Family
Calbiochem®
Application Data
Detection of rat P2X7 by immunoblotting. Samples: Whole tissue extract from rat hippocampus left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-P2X7 Receptor (576-595) Rabbit pAb (Cat. No. 506107) (1:200). Detection: chemiluminescence.
References
References
Collo, G., et al. 1997. Neuropharmacology 36, 1277. Surprenant, A., et al. 1996. Science 272, 735.
Product Information
Form
Lyophilized
Formulation
50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
The immunogen sequence is highly conserved in mouse and human P2X7 (18/20 and 16/20 identical amino acids, respectively). For use of control peptide as a negative control, preincubate 1 µg with 1 µg antibody for 1 h at room temperature. To minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogen
a synthetic peptide [(C)KIRKEFPKTQGQYSGFKYPY] corresponding to amino acids 576-595 of rat P2X7 receptor, conjugated to receptor protein
Immunogen
Rat
Host
Rabbit
Isotype
IgG
Species Reactivity
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute antibody with 50 µl deionized H₂O. Further dilutions should be made in a buffer with a carrier protein such as BSA (1-3%). Dissolve control peptide in deionized water. Reconstitute the control peptide with 100 µl H₂O. Following reconstitution, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number
GTIN
506107-1SET
04055977199437
Documentation
Anti-P2X7 Receptor (576-595) Rabbit pAb Certificates of Analysis
Title
Lot Number
506107
References
Reference overview
Collo, G., et al. 1997. Neuropharmacology 36, 1277. Surprenant, A., et al. 1996. Science 272, 735.
Citations
Title
Marianela Masin, et al. (2006) Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function. Journal of Biological Chemistry281, 4100-4108.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-August-2007 RFH
Application
Immunoblotting (1:200-1:300, chemilimunescence)
Application Data
Detection of rat P2X7 by immunoblotting. Samples: Whole tissue extract from rat hippocampus left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-P2X7 Receptor (576-595) Rabbit pAb (Cat. No. 506107) (1:200). Detection: chemiluminescence.
Description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the P2X7 receptor protein. Supplied with control peptide (immunogen).
Host
Rabbit
Immunogen species
Rat
Immunogen
a synthetic peptide [(C)KIRKEFPKTQGQYSGFKYPY] corresponding to amino acids 576-595 of rat P2X7 receptor, conjugated to receptor protein
Isotype
IgG
Species
rat
Form
Lyophilized
Formulation
50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
Preservative
≤0.1% sodium azide (antibody only)
Comments
The immunogen sequence is highly conserved in mouse and human P2X7 (18/20 and 16/20 identical amino acids, respectively). For use of control peptide as a negative control, preincubate 1 µg with 1 µg antibody for 1 h at room temperature. To minimize non-specific staining, centrifuge all antibody preparations at 10,000 x g for 5 min immediately before use to remove any aggregates. Variables associated with assay conditions will dictate the optimal working dilution.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute antibody with 50 µl deionized H₂O. Further dilutions should be made in a buffer with a carrier protein such as BSA (1-3%). Dissolve control peptide in deionized water. Reconstitute the control peptide with 100 µl H₂O. Following reconstitution, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Collo, G., et al. 1997. Neuropharmacology 36, 1277. Surprenant, A., et al. 1996. Science 272, 735.
Citation
Marianela Masin, et al. (2006) Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function. Journal of Biological Chemistry281, 4100-4108.
