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ABN1675 Anti-Nurr-1

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ABN1675
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MELISA, IH(P), WBRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1675
      DescriptionAnti-Nurr-1
      Alternate Names
      • Nuclear receptor subfamily 4 group A member 2
      • Immediate-early response protein NOT
      • Orphan nuclear receptor NURR1
      • Transcriptionally-inducible nuclear receptor
      • NR4A2
      Background InformationNuclear receptor subfamily 4 group A member 2 (UniProt: P43354; also known as Immediate-early response protein NOT, Orphan nuclear receptor NURR1, Transcriptionally-inducible nuclear receptor) and is encoded by the NR4A2 (also known as NOT, NURR1, TINUR) gene (Gene ID: 4929) in human. Nurr-1 belongs to the nuclear hormone receptor family that acts as a transcriptional regulator and is considered to be important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is also crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. It is expressed in a number of T cell, B cell and fibroblast cell lines, but its expression is higher in brain tissue. Two isoforms of Nurr-1 have been reported that are produced by alternative splicing. The ligand-binding domain (LBD) of Nurr-1 contains no cavity as a result of the tight packing of side chains from several bulky hydrophobic residues in the region normally occupied by ligands. It also lacks a classical binding site for co-activators. However, it has hydrophobic region on the surface of LBD, which is critical for its activity. (Ref.: Volakakis N et al (2006). J. Mol. Endocrinol 37(2); 317-326; Wang G et al. (2003). Nature 423(6939):555-560).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antiserum in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nurr-1, Cat. No. ABN1675, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 4 group A member 2 and has been tested in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Nurr-1 in mouse hippocampus and human cerebellum tissue sections.

      ELISA Analysis: A representative lot detected recombinant Nurr-1 LBDs (Courtesy of Dr. Pierre Leblanc at McLean Hospital, Belmont, MA).

      Immunohistochemistry Analysis: A representative lot detected Nurr-1 in Midbrain dopamine neurons (Leblanc, P., et. al. (2015). J Vis Exp. (102):e52963).

      Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Nurr-1 in HEK293T and CHO cells overexpressed with Myc-Tagged mouse full length Nurr1/Nor1/Nurr77 (Courtesy of Dr. Pierre Leblanc at McLean Hospital, Belmont, MA).

      Immunohistochemistry Analysis: A 1:250-1:2,500 dilution from a representative lot detected Nurr-1 in mouse brain tissue sections.(Courtesy of Dr. Pierre Leblanc at McLean Hospital, Belmont, MA).

      ELISA Analysis: A representative lot detected Nurr-1 ligand binding domain (Leblanc, P., et. al. (2015). J Vis Exp. (102):e52963).

      Western Blotting Analysis: A representative lot detected Nurr-1 in CHO cells transfected with full-length myc-tagged Nurr-1 (Leblanc, P., et. al. (2015). J Vis Exp. (102):e52963).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 271 amino acids from the C-terminal half of human Nurr-1 and includes the ligand binding domain.
      Epitopedomain of:
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Nurr-1 in human and murine tissues. It targets an epitope within the C-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NR4A2
      • NOT
      • NURR1
      • TINUR
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~70 kDa observed; 66.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse cerebral cortex tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Nurr-1 in mouse cerebral cortex tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications