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MAB5352 Anti-Notch 1 Antibody, clone mN1A

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MAB5352
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, FeICC, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5352
      Replaces04-1046
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Notch 1 Antibody, clone mN1A
      Background InformationNotch1 consists of three domains. The extracellular domain containing 36 epidermal growth factor (EGF) repeats and 3 lin-12/Notch repeats (LNR) and functions as a receptor in cell-cell interactions. There is a single transmembrane domain (TM). The intracellular domain contains six cdc10/ankyrin repeats (cdc10) and the newly defined Notch cytokine response (NCR) region, which contains a putative bipartite NLS (nuclear localization signal).
      References
      Product Information
      FormatPurified
      Control
      • In fetal tissues, use spleen, brain stem and lung. In most adult tissues, use lymphoid tissues.
      PresentationPurified immunoglobulin. Liquid.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Notch 1 Antibody, clone mN1A is a high quality Mouse Monoclonal Antibody for the detection of Notch 1 & has been validated in ICC, IHC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot using ECL

      Immunohistochemistry

      Immunocytochemistry

      Immunoprecipitation

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenCdc10-NCR region of mouse Notch1 expressed as a GST fusion protein.
      ClonemN1A
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Notch1, intracellular domain. The antibody has a high affinity for the "activated" intracellular form of Notch1, but a low affinity for the full-length (unprocessed or heterodimeric cell surface) forms. No reactivity with Notch2, 3 or 4.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Rat
      • Feline
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.
      Gene Symbol
      • NOTCH1
      • notch1
      • hN1
      • TAN1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P46531 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity).
      SIZE: 2556 amino acids; 272500 Da
      SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). & Notch 1 intracellular domain: Nucleus (By similarity). Note=Following proteolytical processing NICD is translocated to the nucleus (By similarity).
      TISSUE SPECIFICITY: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
      PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity).
      DISEASE: SwissProt: P46531 # NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia. & Defects in NOTCH1 are a cause of aortic valve disease [MIM:109730]. The disorder consists of an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease. Calcification of the aortic valve is the third leading cause of heart disease in adults. The incidence increases with age, and it is often associated with a bicuspid aortic valve present in 1-2% of the population.
      SIMILARITY: SwissProt: P46531 ## Belongs to the NOTCH family. & Contains 5 ANK repeats. & Contains 36 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.
      Molecular Weight272.5 kDa (also 230 kDa processed form)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Notch 1 Antibody, clone mN1A SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Notch 1 Antibody, clone mN1A Certificates of Analysis

      TitleLot Number
      Anti-Notch1, clone mN1A Monoclonal Antibody Q2925513
      MOUSE ANTI-MOUSE NOTCH1 MONOCLONAL ANTIBODY - 2378672 2378672
      MOUSE ANTI-MOUSE NOTCH1 - 2495871 2495871
      MOUSE ANTI-MOUSE NOTCH1 - 3837165 3837165
      MOUSE ANTI-MOUSE NOTCH1 -2554880 2554880
      MOUSE ANTI-MOUSE NOTCH1 -2594368 2594368
      MOUSE ANTI-MOUSE NOTCH1 -2619292 2619292
      MOUSE ANTI-MOUSE NOTCH1 -2631127 2631127
      MOUSE ANTI-MOUSE NOTCH1 -2793624 2793624
      MOUSE ANTI-MOUSE NOTCH1 MONOCLONAL ANTIBODY 3158915

      References

      Reference overviewPub Med ID
      MDM2 protein-mediated ubiquitination of numb protein: identification of a second physiological substrate of MDM2 that employs a dual-site docking mechanism.
      Sczaniecka, M; Gladstone, K; Pettersson, S; McLaren, L; Huart, AS; Wallace, M
      The Journal of biological chemistry  287  14052-68  2012

      Show Abstract
      22337874 22337874
      Notch1 in primary effusion lymphoma: a clinicopathological study.
      Wang, Huan-You, et al.
      Mod. Pathol., 23: 773-80 (2010)  2010

      Show Abstract
      20348880 20348880
      Activation of the Notch pathway in Down syndrome: cross-talk of Notch and APP.
      Fischer, DF; van Dijk, R; Sluijs, JA; Nair, SM; Racchi, M; Levelt, CN; van Leeuwen, FW; Hol, EM
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  19  1451-8  2005

      Show Abstract
      16126912 16126912
      Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1.
      Huppert, S S, et al.
      Nature, 405: 966-70 (2000)  2000

      10879540 10879540
      Inhibition of granulocytic differentiation by mNotch1.
      Milner, L A, et al.
      Proc. Natl. Acad. Sci. U.S.A., 93: 13014-9 (1996)  1996

      Show Abstract
      8917536 8917536