MAB5460 Anti-Nkx2.1 Antibody, clone 8G7-G3-1

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) M Ascites Monoclonal Antibody
      Catalogue NumberMAB5460
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nkx2.1 Antibody, clone 8G7-G3-1
      Alternate Names
      • TTF-1
      • Thyroid Transcription Factor-1
      Product Information
      PresentationAscites fluid. Liquid. Contains no preservative.
      Quality LevelMQ100
      ApplicationThis Anti-Nkx2.1 Antibody is validated for use in WB, IH(P) for the detection of Nkx2.1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot. The antibody reacts with a 40 kDa band in immunoblots of nuclear extracts or whole cell lysates from the Nkx2.1 positive cell lines MLE15, H441-4, H345 and rat type II pmeumocyte cells.

      Immunohistochemistry. The antibody can be used on formalin-fixed paraffin embedded tissue. Heat induced epitope retrieval is recommended.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant rat Nkx2.1.
      SpecificityNkx2.1 (Thyroid Transcription Factor-1, TTF-1).
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NKX2-1
      • BCH
      • TEBP
      • TITF1
      • TTF1
      • NKX2.1
      • NK-2
      • TTF-1
      • BHC
      • NKX2A
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P43699 # Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
      SIZE: 371 amino acids; 38596 Da
      TISSUE SPECIFICITY: Thyroid and lung.
      PTM: Phosphorylated on serine residues (By similarity).
      DISEASE: SwissProt: P43699 # Defects in TITF1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances. & Defects in TITF1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.
      SIMILARITY: SwissProt: P43699 ## Belongs to the NK-2 homeobox family. & Contains 1 homeobox DNA-binding domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-Nkx2.1 Antibody, clone 8G7-G3-1 SDS


      Safety Data Sheet (SDS) 

      Anti-Nkx2.1 Antibody, clone 8G7-G3-1 Certificates of Analysis

      TitleLot Number
      MAB5460 - 24501952450195
      MAB5460 - 22795432279543
      MOUSE ANTI-NKX2.1 - 25103292510329
      MOUSE ANTI-NKX2.1 - 31601393160139
      MOUSE ANTI-NKX2.1 - 33059253305925
      MOUSE ANTI-NKX2.1 -26975932697593
      MOUSE ANTI-NKX2.1 -27102632710263
      MOUSE ANTI-NKX2.1 -28220492822049


      Reference overviewApplicationPub Med ID
      Grainyhead-like 2 (GRHL2) distribution reveals novel pathophysiological differences between human idiopathic pulmonary fibrosis and mouse models of pulmonary fibrosis.
      Varma, S; Mahavadi, P; Sasikumar, S; Cushing, L; Hyland, T; Rosser, AE; Riccardi, D; Lu, J; Kalin, TV; Kalinichenko, VV; Guenther, A; Ramirez, MI; Pardo, A; Selman, M; Warburton, D
      American journal of physiology. Lung cellular and molecular physiology  306  L405-19  2014

      Show Abstract
      24375798 24375798
      The specification of telencephalic glutamatergic neurons from human pluripotent stem cells.
      Boisvert, Erin M, et al.
      J Vis Exp, (2013)  2013

      Show Abstract
      23603787 23603787
      Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits.
      Liu, Y; Weick, JP; Liu, H; Krencik, R; Zhang, X; Ma, L; Zhou, GM; Ayala, M; Zhang, SC
      Nature biotechnology  31  440-7  2013

      Show Abstract
      23604284 23604284
      Directed differentiation of forebrain GABA interneurons from human pluripotent stem cells.
      Liu, Y; Liu, H; Sauvey, C; Yao, L; Zarnowska, ED; Zhang, SC
      Nature protocols  8  1670-9  2013

      Show Abstract
      Immunocytochemistry23928500 23928500

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